LOCUS       pMA3790                 7117 bp    DNA     circular     20-SEP-2013
DEFINITION  2008+EF1a-EGFP + MTS-UNG147A.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|673633451|
COMMENT     VNTDBDATE|673633451|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMA3790|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTAUTHOREML|dh|
COMMENT     VNTAUTHORFTP|hgfh|
COMMENT     VNTAUTHORWWW|mcv|
COMMENT     VNTAUTHORAD1|vcm|
COMMENT     VNTAUTHORAD2|mcbh|
FEATURES             Location/Qualifiers
     CDS             612..707
                     /vntifkey="4"
                     /label=MTS-hOTC-Koz
     CDS             708..737
                     /vntifkey="4"
                     /label=myc-Tag\corrected
     CDS             744..1427
                     /gene="UNG"
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /product="uracil-DNA glycosylase isoform UNG1 precursor"
                     /protein_id="NP_003353.1"
                     /db_xref="GI:6224979"
                     /db_xref="GeneID:7374"
                     /db_xref="LocusID:7374"
                     /db_xref="MIM:191525"
                     /vntifkey="4"
                     /label=UNG\Y147A
                     /note="isoform UNG1 precursor is encoded by transcript variant 1; uracil-DNA glycosylase 1; go_component: mitochondrion [goid 0005739] [evidence IEA]; go_component: nucleus [goid 0005634] [evidence IEA]; go_function: uracil DNA N-glycosylase activity [goid 0004844] [evidence TAS]; go_function: hydrolase activity, acting on glycosyl bonds [goid 0016798] [evidence IEA]; go_process: base-excision repair [goid 0006284] [evidence TAS]; go_process: DNA repair [goid 0006281] [evidence E] [pmid 1923798]; go_process: carbohydrate metabolism [goid 0005975] [evidence IEA]"
     CDS             5013..5807
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB08059.1"
                     /db_xref="GI:1373317"
                     /vntifkey="4"
                     /label=neoR
     promoter        1..589
                     /vntifkey="29"
                     /label=Pcmv-human
     rep_origin      6392..7035
                     /vntifkey="33"
                     /label=ColE1ori
     promoter        4662..4891
                     /vntifkey="29"
                     /label=Psv40
     rep_origin      4829..4964
                     /vntifkey="33"
                     /label=SV40ori
     polyA_signal    5982..6252
                     /vntifkey="25"
                     /label=HSVTk\polyA
     promoter        4550..4584
                     /vntifkey="30"
                     /label=Km-prom
     polyA_signal    1439..1667
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      4033..4488
                     /vntifkey="33"
                     /label=f1\ori
     polyA_signal    complement(1809..1949)
                     /vntifkey="25"
                     /label=BGHpA\BLAST\corrected
     intron          complement(2849..3791)
                     /vntifkey="15"
                     /label=Intron
     CDS             complement(2099..2818)
                     /vntifkey="4"
                     /label=EGFP
     promoter        complement(2841..4024)
                     /vntifkey="29"
                     /label=EF1a(1a)
BASE COUNT     1664 a      1933 c      1903 g      1617 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagatcga 
      601 attccgccac catgctgttt aatctgagga ttctgttaaa caatgcagct tttagaaatg 
      661 gtcacaactt catggttcga aattttcggt gtggacaacc actacaagag cagaaactca 
      721 tcagcgagga ggacctggga tctgcccgca acgtgcccgt gggctttgga gagagctgga 
      781 agaagcacct cagcggggag ttcgggaaac cgtattttat caagctaatg ggatttgttg 
      841 cagaagaaag aaagcattac actgtttatc cacccccaca ccaagtcttc acctggaccc 
      901 agatgtgtga cataaaagat gtgaaggttg tcatcctggg acaggatcca gcccatggac 
      961 ctaatcaagc tcacgggctc tgctttagtg ttcaaaggcc tgttccgcct ccgcccagtt 
     1021 tggagaacat ttataaagag ttgtctacag acatagagga ttttgttcat cctggccatg 
     1081 gagatttatc tgggtgggcc aagcaaggtg ttctccttct caacgctgtc ctcacggttc 
     1141 gtgcccatca agccaactct cataaggagc gaggctggga gcagttcact gatgcagttg 
     1201 tgtcctggct aaatcagaac tcgaatggcc ttgttttctt gctctggggc tcttatgctc 
     1261 agaagaaggg cagtgccatt gataggaagc ggcaccatgt actacagacg gctcatccct 
     1321 cccctttgtc agtgtataga gggttctttg gatgtagaca cttttcaaag accaatgagc 
     1381 tgctgcagaa gtctggcaag aagcccattg actggaagga gctgtgagtc tagataactg 
     1441 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 
     1501 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 
     1561 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     1621 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgcgtcaatt 
     1681 cgaggggctg caggaattcg atctggttct ttccgcctca gaagccatag agcccaccgc 
     1741 atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc 
     1801 accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta 
     1861 ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac 
     1921 aacagatggc tggcaactag aaggcacagt cgaggctgat cagcgagctc tagcatttag 
     1981 gtgacactat agaatagggc cctctagaac tagtaggcct attatttttg acaccagaca 
     2041 agttggtaat ggtagcgacc ggcgctcagt tggaattcta gagtcgcggc cggccgcttt 
     2101 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga 
     2161 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt 
     2221 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg 
     2281 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt 
     2341 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt 
     2401 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca 
     2461 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga 
     2521 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct 
     2581 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg 
     2641 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt 
     2701 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt 
     2761 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg 
     2821 tggcgaccgg taccaagctt cacgacacct gaaatggaag aaaaaaactt tgaaccactg 
     2881 tctgaggctt gagaatgaac caagatccaa actcaaaaag ggcaaattcc aaggagaatt 
     2941 acatcaagtg ccaagctggc ctaacttcag tctccaccca ctcagtgtgg ggaaactcca 
     3001 tcgcataaaa cccctccccc caacctaaag acgacgtact ccaaaagctc gagaactaat 
     3061 cgaggtgcct ggacggcgcc cggtactccg tggagtcaca tgaagcgacg gctgaggacg 
     3121 gaaaggccct tttcctttgt gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc 
     3181 tccattttga gctccctgca gcagggccgg gaagcggcca tctttccgct cacgcaactg 
     3241 gtgccgaccg ggccagcctt gccgcccagg gcggggcgat acacggcggc gcgaggccag 
     3301 gcaccagagc aggccggcca gcttgagact acccccgtcc gattctcggt ggccgcgctc 
     3361 gcaggccccg cctcgccgaa catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg 
     3421 ccccaaaaac cgaaatacca gtgtgcagat cttggcccgc atttacaaga ctatcttgcc 
     3481 agaaaaaaag cgtcgcagca ggtcatcaaa aattttaaat ggctagagac ttatcgaaag 
     3541 cagcgagaca ggcgcgaagg tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc 
     3601 aggcctcaac tcaagcacga ggcgaagggg ctccttaagc gcaaggcctc gaactctccc 
     3661 acccacttcc aacccgaagc tcgggatcaa gaatcacgta ctgcagccag gggcgtggaa 
     3721 gtaattcaag gcacgcaagg gccataaccc gtaaagaggc caggcccgcg ggaaccacac 
     3781 acggcactta cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact 
     3841 actgcactta tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca 
     3901 tcactttccc agtttacccc gcgccacctt ctctaggcac cggttcaatt gccgacccct 
     3961 ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccactgacg ggcaccggag 
     4021 cctcacgcgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt 
     4081 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 
     4141 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 
     4201 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 
     4261 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 
     4321 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 
     4381 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 
     4441 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt 
     4501 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 
     4561 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc 
     4621 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 
     4681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 
     4741 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 
     4801 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     4861 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     4921 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga 
     4981 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 
     5041 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 
     5101 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 
     5161 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc 
     5221 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 
     5281 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 
     5341 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 
     5401 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 
     5461 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 
     5521 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg 
     5581 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 
     5641 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 
     5701 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 
     5761 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 
     5821 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 
     5881 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 
     5941 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac 
     6001 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa 
     6061 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc 
     6121 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc 
     6181 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg 
     6241 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta 
     6301 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
     6361 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     6421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     6481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     6541 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 
     6601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     6661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     6721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     6781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 
     6841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     6901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     6961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     7021 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
     7081 ccctgattct gtggataacc gtattaccgc catgcat 
//
