LOCUS       pEMS2158        4379 bp    DNA     circular      9-SEP-2015
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|741437794|
COMMENT     VNTDBDATE|744564651|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2158|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|User|
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COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2081894036 
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FEATURES             Location/Qualifiers
     CDS             2332..3189
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    3363..3951
                     /vntifkey="21"
                     /label=Origin
     misc_feature    1394..1439
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(1402..1569)
                     /vntifkey="34"
                     /label=3'\ITR
     misc_feature    197..208
                     /vntifkey="21"
                     /label=MCS\5'\end
     intron          223..395
                     /vntifkey="15"
                     /label=Intron
     misc_feature    404..1127
                     /vntifkey="21"
                     /label=emGFP
BASE COUNT     1071 a      1160 c      1125 g      1023 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggccac gcgtggcgcg ccgaagttgg tcgtgaggca 
      241 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 
      301 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 
      361 actttgcctt tctctccaca ggtgtccact cccaggcggc cgccaccatg gtgagcaagg 
      421 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 
      481 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 
      541 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct 
      601 tcacctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct 
      661 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 
      721 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 
      781 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 
      841 actacaacag ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga 
      901 acttcaagac ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 
      961 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 
     1021 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 
     1081 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagcg gccgcgatcg 
     1141 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 
     1201 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 
     1261 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt 
     1321 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tagtcgaaat tcccgataag 
     1381 gatcttccta gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa 
     1441 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc 
     1501 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg 
     1561 agcgcgcagc cttaattaac ctaattcact ggccgtcgtt ttacaacgtc gtgactggga 
     1621 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 
     1681 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 
     1741 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 
     1801 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 
     1861 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 
     1921 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 
     1981 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 
     2041 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 
     2101 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 
     2161 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga 
     2221 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 
     2281 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 
     2341 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 
     2401 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 
     2461 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 
     2521 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 
     2581 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 
     2641 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 
     2701 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 
     2761 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 
     2821 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 
     2881 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 
     2941 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 
     3001 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 
     3061 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 
     3121 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 
     3181 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 
     3241 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 
     3301 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 
     3361 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 
     3421 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 
     3481 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 
     3541 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 
     3601 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 
     3661 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 
     3721 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
     3781 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
     3841 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
     3901 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
     3961 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 
     4021 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 
     4081 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 
     4141 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 
     4201 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 
     4261 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 
     4321 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccagatt taattaagg 
//
