LOCUS       pEMS2159        5284 bp    DNA     circular      9-SEP-2015
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|741976243|
COMMENT     VNTDBDATE|744561515|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2159|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|User|
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FEATURES             Location/Qualifiers
     CDS             3237..4094
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    4268..4856
                     /vntifkey="21"
                     /label=Origin
     misc_feature    2299..2344
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(2307..2474)
                     /vntifkey="34"
                     /label=3'\ITR
     misc_feature    197..208
                     /vntifkey="21"
                     /label=MCS\5'\end
     intron          223..395
                     /vntifkey="15"
                     /label=Intron
     CDS             408..1439
                     /vntifkey="4"
                     /label=iCre
     misc_feature    1454..2040
                     /vntifkey="21"
                     /label=WPRE
     primer          complement(424..444)
                     /vntifkey="27"
                     /label=oEMS5913
     primer          complement(542..563)
                     /vntifkey="27"
                     /label=oEMS5917
BASE COUNT     1205 a      1389 c      1376 g      1314 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggccac gcgtggcgcg ccgaagttgg tcgtgaggca 
      241 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 
      301 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 
      361 actttgcctt tctctccaca ggtgtccact cccaggcggc cgccaccatg tccaacctgc 
      421 tgactgtgca ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga 
      481 agaacctgat ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc 
      541 tcctgtctgt gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc 
      601 ctgctgaacc tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg 
      661 tgaagaccat ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc 
      721 ctcgcccttc tgactccaat gctgtgtccc tggtgatgag gagaatcaga aaggagaatg 
      781 tggatgctgg ggagagagcc aagcaggccc tggcctttga acgcactgac tttgaccaag 
      841 tcagatccct gatggagaac tctgacagat gccaggacat caggaacctg gccttcctgg 
      901 gcattgccta caacaccctg ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca 
      961 tctcccgcac cgatggtggg agaatgctga tccacattgg caggaccaag accctggtgt 
     1021 ccacagctgg tgtggagaag gccctgtccc tgggggttac caagctggtg gagagatgga 
     1081 tctctgtgtc tggtgtggct gatgacccca acaactacct gttctgccgg gtcagaaaga 
     1141 atggtgtggc tgccccttct gccacctccc aactgtccac ccgggccctg gaagggatct 
     1201 ttgaggccac ccaccgcctg atctatggtg ccaaggatga ctctgggcag agatacctgg 
     1261 cctggtctgg ccactctgcc agagtgggtg ctgccaggga catggccagg gctggtgtgt 
     1321 ccatccctga aatcatgcag gctggtggct ggaccaatgt gaacatagtg atgaactaca 
     1381 tcagaaacct ggactctgag actggggcca tggtgaggct gctcgaggat ggggactgag 
     1441 cggccgcgat cgctcaacct ctggattaca aaatttgtga aagattgact ggtattctta 
     1501 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 
     1561 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 
     1621 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 
     1681 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 
     1741 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 
     1801 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 
     1861 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 
     1921 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 
     1981 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 
     2041 gatcgcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 
     2101 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 
     2161 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 
     2221 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtagtc gaaattcccg 
     2281 ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt aatcattaac 
     2341 tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact 
     2401 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc 
     2461 gagcgagcgc gcagccttaa ttaacctaat tcactggccg tcgttttaca acgtcgtgac 
     2521 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 
     2581 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 
     2641 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
     2701 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 
     2761 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 
     2821 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 
     2881 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
     2941 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 
     3001 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 
     3061 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc 
     3121 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 
     3181 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 
     3241 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 
     3301 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 
     3361 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 
     3421 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 
     3481 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 
     3541 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 
     3601 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 
     3661 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 
     3721 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 
     3781 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 
     3841 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 
     3901 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 
     3961 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 
     4021 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 
     4081 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 
     4141 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     4201 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     4261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     4321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     4381 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 
     4441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     4501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     4561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     4621 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     4681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     4741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     4801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     4861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     4921 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
     4981 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 
     5041 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 
     5101 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 
     5161 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 
     5221 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc agatttaatt 
     5281 aagg 
//
