LOCUS       pRERTOX-TIGHT-GF        7150 bp ss-DNA     circular SYN 22-Nov-2011
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     LTR             1..727
                     /note="5' LTR CMV"
     misc_feature    759..1568
                     /note="Psi"
     primer_bind     1498..1520
                     /note="FS 49_Forward Seq Primer"
     promoter        1603..1918
                     /note="Ptight"
     primer_bind     1853..1870
                     /note="FS 90_Tight_Forward Seq Primer"
     misc_feature    1926..1969
                     /note="MCS"
     promoter        1970..2473
                     /note="PgK"
     primer_bind     2044..2063
                     /note="FS 91_Reverse Seq Primer"
     primer_bind     2311..2328
                     /note="FS 16_Forward Seq Primer"
     CDS             2494..3210
                     /note="eGFP"
     primer_bind     3142..3159
                     /note="Tg Primer_F"
     misc_feature    3211..3225
                     /note="5 amino acid Linker"
     gene            3226..3741
                     /note="NanoLuc"
     primer_bind     3286..3303
                     /note="Tg Primer_R"
     LTR             3855..4280
                     /note="3' LTR"
     primer_bind     3859..3877
                     /note="FS 52_Reverse Seq Primer"
BASE COUNT      1670 A      1962 C      1845 G      1673 T         0 OTHER
ORIGIN      ?
        1 TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC
       61 CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC
      121 GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA
      181 TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCTCTG GCATTATGCC
      241 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT
      301 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA
      361 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT
      421 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG
      481 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCAATAAA AGAGCCCACA ACCCCTCACT
      541 CGGCGCGCCA GTCTTCCGAT AGACTGCGTC GCCCGGGTAC CCGTATTCCC AATAAAGCCT
      601 CTTGCTGTTT GCATCCGAAT CGTGGTCTCG CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA
      661 TTGACTACCC ACGACGGGGG TCTTTCATTT GGGGGCTCGT CCGGGATTTG GAGACCCCTG
      721 CCCAGGGACC ACCGACCCAC CACCGGGAGG TAAGCTGGCC AGCAACTTAT CTGTGTCTGT
      781 CCGATTGTCT AGTGTCTATG TTTGATGTTA TGCGCCTGCG TCTGTACTAG TTAGCTAACT
      841 AGCTCTGTAT CTGGCGGACC CGTGGTGGAA CTGACGAGTT CTGAACACCC GGCCGCAACC
      901 CTGGGAGACG TCCCAGGGAC TTTGGGGGCC GTTTTTGTGG CCCGACCTGA GGAAGGGAGT
      961 CGATGTGGAA TCCGACCCCG TCAGGATATG TGGTTCTGGT AGGAGACGAG AACCTAAAAC
     1021 AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC
     1081 TGCTGCAGCG CTGCAGCATC GTTCTGTGTT GTCTCTGTCT GACTGTGTTT CTGTATTTGT
     1141 CTGAAAATTA GGGCCAGACT GTTACCACTC CCTTAAGTTT GACCTTAGGT CACTGGAAAG
     1201 ATGTCGAGCG GATCGCTCAC AACCAGTCGG TAGATGTCAA GAAGAGACGT TGGGTTACCT
     1261 TCTGCTCTGC AGAATGGCCA ACCTTTAACG TCGGATGGCC GCGAGACGGC ACCTTTAACC
     1321 GAGACCTCAT CACCCAGGTT AAGATCAAGG TCTTTTCACC TGGCCCGCAT GGACACCCAG
     1381 ACCAGGTCCC CTACATCGTG ACCTGGGAAG CCTTGGCTTT TGACCCCCCT CCCTGGGTCA
     1441 AGCCCTTTGT ACACCCTAAG CCTCCGCCTC CTCTTCCTCC ATCCGCCCCG TCTCTCCCCC
     1501 TTGAACCTCC TCGTTCGACC CCGCCTCGAT CCTCCCTTTA TCCAGCCCTC ACTCCTTCTC
     1561 TAGGCGCCGG AATTGAAGAT CTGAGGCCCT TTCGTCTTCA CTCGAGTTTA CTCCCTATCA
     1621 GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGA TGTCGAGTTT
     1681 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTACTC CCTATCAGTG ATAGAGAACG
     1741 TATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAT CCCTATCAGT
     1801 GATAGAGAAC GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GTCGAGGTAG
     1861 GCGTGTACGG TGGGAGGCCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG
     1921 GAGAAGGATC CGCGGCCGCG CCGGCTCTAG ATCGCGAACG CGTGAATTCT ACCGGGTAGG
     1981 GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG
     2041 GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC
     2101 GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT
     2161 CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC
     2221 TAGTCTCGTG CAGATGGACA GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG
     2281 CGGCCAATAG CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC
     2341 CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG
     2401 CCCGGCATTC TGCACGCTTC AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC
     2461 TCCGGGCCTT TCGACCTGCA GCCCAAGGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC
     2521 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC
     2581 GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC
     2641 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG
     2701 CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG
     2761 CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC
     2821 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC
     2881 GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC
     2941 AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC
     3001 CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC
     3061 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC
     3121 AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG
     3181 ATCACTCTCG GCATGGACGA GCTGTACAAG GATATCTCCG GAGGTATGGT CTTCACACTC
     3241 GAAGATTTCG TTGGGGACTG GCGACAGACA GCCGGCTACA ACCTGGACCA AGTCCTTGAA
     3301 CAGGGAGGTG TGTCCAGTTT GTTTCAGAAT CTCGGGGTGT CCGTAACTCC GATCCAAAGG
     3361 ATTGTCCTGA GCGGTGAAAA TGGGCTGAAG ATCGACATCC ATGTCATCAT CCCGTATGAA
     3421 GGTCTGAGCG GCGACCAAAT GGGCCAGATC GAAAAAATTT TTAAGGTGGT GTACCCTGTG
     3481 GATGATCATC ACTTTAAGGT GATCCTGCAC TATGGCACAC TGGTAATCGA CGGGGTTACG
     3541 CCGAACATGA TCGACTATTT CGGACGGCCG TATGAAGGCA TCGCCGTGTT CGACGGCAAA
     3601 AAGATCACTG TAACAGGGAC CCTGTGGAAC GGCAACAAAA TTATCGACGA GCGCCTGATC
     3661 AACCCCGACG GCTCCCTGCT GTTCCGAGTA ACCATCAACG GAGTGACCGG CTGGCGGCTG
     3721 TGCGAACGCA TTCTGGCGTA AATCGATAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA
     3781 TCACCAGCTG AAGCCTATAG AGTACGAGCC ATAGATAAAA TAAAAGATTT TATTTAGTCT
     3841 CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC
     3901 GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTC
     3961 AGGAACAGAT GGAACAGGGT CGACCCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA
     4021 GGACCTGAAA TGACCCTGTG CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG
     4081 TTCGCGCGCT TCTGCTCCCC GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC
     4141 CAGTCCTCCG ATTGACTGAG TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT
     4201 TGCATCCGAC TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC
     4261 CGTCAGCGGG GGTCTTTCAT TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA
     4321 CCACCGACCC ACCACCGGGA GGTAAGCTGG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA
     4381 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG
     4441 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT
     4501 GACCCAGTCA CGTAGCGATA GCGGAGTGTA GATCCGGCTG TGGAATGTGT GTCAGTTAGG
     4561 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA
     4621 GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT
     4681 GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC
     4741 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA
     4801 GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG
     4861 CCTAGGCTTT TGCAAAAAGC TTACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG
     4921 AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC
     4981 AGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA
     5041 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA
     5101 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
     5161 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
     5221 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
     5281 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
     5341 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
     5401 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
     5461 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
     5521 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
     5581 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG
     5641 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
     5701 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
     5761 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     5821 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     5881 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     5941 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     6001 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     6061 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     6121 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     6181 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     6241 GCTGCAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     6301 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     6361 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     6421 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     6481 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     6541 TCAACACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     6601 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     6661 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     6721 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     6781 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     6841 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT
     6901 CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA
     6961 AATAGGCGTA TCACGAGGCC CTTTCGTCTT CAAGAATTAG CTTGGCCATT GCATACGTTG
     7021 TATCCATATC ATAATATGTA CATTTATATT GGCTCATGTC CAACATTACC GCCATGTTGA
     7081 CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA
     7141 TATATGGAGT
//
