LOCUS       pRERTOX-TIGHT-GF        7141 bp ss-DNA     circular SYN 22-Nov-2011
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     LTR             1..727
                     /note="5' LTR CMV"
     misc_feature    759..1568
                     /note="Psi"
     primer_bind     1498..1520
                     /note="FS 49_Forward Seq Primer"
     promoter        1603..1918
                     /note="Ptight"
     primer_bind     1853..1870
                     /note="FS 90_Tight_Forward Seq Primer"
     misc_feature    1926..1969
                     /note="MCS"
     promoter        1970..2473
                     /note="PgK"
     primer_bind     2044..2063
                     /note="FS 91_Reverse Seq Primer"
     primer_bind     2311..2328
                     /note="FS 16_Forward Seq Primer"
     frag            2494..3201
                     /date="17-Sep-2014"
                     /note="1 to 708 of Untitled"
     CDS             2494..3201
                     /note="LSSmOrange"
     misc_feature    3202..3216
                     /note="5 amino acid Linker"
     gene            3217..3732
                     /note="NanoLuc"
     LTR             3846..4271
                     /note="3' LTR"
     primer_bind     3850..3868
                     /note="FS 52_Reverse Seq Primer"
BASE COUNT      1658 A      1947 C      1858 G      1678 T         0 OTHER
ORIGIN      ?
        1 TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC
       61 CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC
      121 GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA
      181 TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCTCTG GCATTATGCC
      241 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT
      301 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA
      361 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT
      421 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG
      481 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCAATAAA AGAGCCCACA ACCCCTCACT
      541 CGGCGCGCCA GTCTTCCGAT AGACTGCGTC GCCCGGGTAC CCGTATTCCC AATAAAGCCT
      601 CTTGCTGTTT GCATCCGAAT CGTGGTCTCG CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA
      661 TTGACTACCC ACGACGGGGG TCTTTCATTT GGGGGCTCGT CCGGGATTTG GAGACCCCTG
      721 CCCAGGGACC ACCGACCCAC CACCGGGAGG TAAGCTGGCC AGCAACTTAT CTGTGTCTGT
      781 CCGATTGTCT AGTGTCTATG TTTGATGTTA TGCGCCTGCG TCTGTACTAG TTAGCTAACT
      841 AGCTCTGTAT CTGGCGGACC CGTGGTGGAA CTGACGAGTT CTGAACACCC GGCCGCAACC
      901 CTGGGAGACG TCCCAGGGAC TTTGGGGGCC GTTTTTGTGG CCCGACCTGA GGAAGGGAGT
      961 CGATGTGGAA TCCGACCCCG TCAGGATATG TGGTTCTGGT AGGAGACGAG AACCTAAAAC
     1021 AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC
     1081 TGCTGCAGCG CTGCAGCATC GTTCTGTGTT GTCTCTGTCT GACTGTGTTT CTGTATTTGT
     1141 CTGAAAATTA GGGCCAGACT GTTACCACTC CCTTAAGTTT GACCTTAGGT CACTGGAAAG
     1201 ATGTCGAGCG GATCGCTCAC AACCAGTCGG TAGATGTCAA GAAGAGACGT TGGGTTACCT
     1261 TCTGCTCTGC AGAATGGCCA ACCTTTAACG TCGGATGGCC GCGAGACGGC ACCTTTAACC
     1321 GAGACCTCAT CACCCAGGTT AAGATCAAGG TCTTTTCACC TGGCCCGCAT GGACACCCAG
     1381 ACCAGGTCCC CTACATCGTG ACCTGGGAAG CCTTGGCTTT TGACCCCCCT CCCTGGGTCA
     1441 AGCCCTTTGT ACACCCTAAG CCTCCGCCTC CTCTTCCTCC ATCCGCCCCG TCTCTCCCCC
     1501 TTGAACCTCC TCGTTCGACC CCGCCTCGAT CCTCCCTTTA TCCAGCCCTC ACTCCTTCTC
     1561 TAGGCGCCGG AATTGAAGAT CTGAGGCCCT TTCGTCTTCA CTCGAGTTTA CTCCCTATCA
     1621 GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGA TGTCGAGTTT
     1681 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTACTC CCTATCAGTG ATAGAGAACG
     1741 TATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAT CCCTATCAGT
     1801 GATAGAGAAC GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GTCGAGGTAG
     1861 GCGTGTACGG TGGGAGGCCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG
     1921 GAGAAGGATC CGCGGCCGCG CCGGCTCTAG ATCGCGAACG CGTGAATTCT ACCGGGTAGG
     1981 GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG
     2041 GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC
     2101 GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT
     2161 CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC
     2221 TAGTCTCGTG CAGATGGACA GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG
     2281 CGGCCAATAG CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC
     2341 CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG
     2401 CCCGGCATTC TGCACGCTTC AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC
     2461 TCCGGGCCTT TCGACCTGCA GCCCAAGGCC ACCATGGTGA GCAAGGGCGA GGAGAATAAC
     2521 ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCGCA TGGAGGGCTC CGTGAACGGC
     2581 CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCTT TCAGACCGTT
     2641 AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCTT GTCCCCTCAG
     2701 TTCACCTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACCTCAAG
     2761 CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG
     2821 GTGACCGTGA CTCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG
     2881 CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCATGGAG
     2941 GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGGA CAAGCTCAGG
     3001 CTGAAGCTGA AGGACGGCGG CCACTACACC TCCGAGGTCA AGACCACCTA CAAGGCCAAG
     3061 AAGCCCGTGC AGTTGCCCGG CGCCTACATC GTCGACATCA AGTTGGACAT CACCTCCCAC
     3121 AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC
     3181 GGCATGGACG AGCTGTACAA GGATATCTCC GGAGGTATGG TCTTCACACT CGAAGATTTC
     3241 GTTGGGGACT GGCGACAGAC AGCCGGCTAC AACCTGGACC AAGTCCTTGA ACAGGGAGGT
     3301 GTGTCCAGTT TGTTTCAGAA TCTCGGGGTG TCCGTAACTC CGATCCAAAG GATTGTCCTG
     3361 AGCGGTGAAA ATGGGCTGAA GATCGACATC CATGTCATCA TCCCGTATGA AGGTCTGAGC
     3421 GGCGACCAAA TGGGCCAGAT CGAAAAAATT TTTAAGGTGG TGTACCCTGT GGATGATCAT
     3481 CACTTTAAGG TGATCCTGCA CTATGGCACA CTGGTAATCG ACGGGGTTAC GCCGAACATG
     3541 ATCGACTATT TCGGACGGCC GTATGAAGGC ATCGCCGTGT TCGACGGCAA AAAGATCACT
     3601 GTAACAGGGA CCCTGTGGAA CGGCAACAAA ATTATCGACG AGCGCCTGAT CAACCCCGAC
     3661 GGCTCCCTGC TGTTCCGAGT AACCATCAAC GGAGTGACCG GCTGGCGGCT GTGCGAACGC
     3721 ATTCTGGCGT AAATCGATAG TGGTCCAGGC TCTAGTTTTG ACTCAACAAT ATCACCAGCT
     3781 GAAGCCTATA GAGTACGAGC CATAGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA
     3841 GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG
     3901 CAAGGCATGG AAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT CAGGAACAGA
     3961 TGGAACAGGG TCGACCCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA
     4021 ATGACCCTGT GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC
     4081 TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGGGCG CCAGTCCTCC
     4141 GATTGACTGA GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA
     4201 CTTGTGGTCT CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG
     4261 GGGTCTTTCA TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC
     4321 CACCACCGGG AGGTAAGCTG GCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG
     4381 ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA
     4441 AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC
     4501 ACGTAGCGAT AGCGGAGTGT AGATCCGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA
     4561 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC
     4621 CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA
     4681 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG
     4741 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC
     4801 CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT
     4861 TTGCAAAAAG CTTACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC
     4921 CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT
     4981 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA
     5041 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC
     5101 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
     5161 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
     5221 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
     5281 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
     5341 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
     5401 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
     5461 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
     5521 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
     5581 AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
     5641 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
     5701 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
     5761 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     5821 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     5881 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     5941 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     6001 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     6061 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     6121 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     6181 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTGCAGGC
     6241 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     6301 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     6361 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     6421 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     6481 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAACACGG
     6541 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     6601 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     6661 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     6721 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     6781 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     6841 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
     6901 GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT
     6961 ATCACGAGGC CCTTTCGTCT TCAAGAATTA GCTTGGCCAT TGCATACGTT GTATCCATAT
     7021 CATAATATGT ACATTTATAT TGGCTCATGT CCAACATTAC CGCCATGTTG ACATTGATTA
     7081 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG
     7141 T
//
