LOCUS       pAP24                   7813 bp    DNA     circular SYN 05-OCT-2015
DEFINITION  Modular plasmid for C. difficile with secreted luciferase reporter.
ACCESSION   pAP24
KEYWORDS    .
SOURCE      Unknown
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Paiva,A., Friggen,A., Javaheri,S., Smits, W.K.
  JOURNAL   Unpublished.
COMMENT     
FEATURES             Location/Qualifiers
     CDS             24..620
                     /gene="sLucopt"
     primer_bind     complement(857..879)
                     /Sequence="CACCGACGAGCAAGGCAAGACCG"
                     /Hairpin_Tm="46.5"
                     /Tm="68.2"
                     /Self_Dimer_Tm="None"
                     /%GC="65.2"
                     /created_by="primer3"
                     /label="NF_794"
                     /note="Geneious type: primer_bind_reverse"
     CDS             complement(982..1353)
                     /created_by="User"
                     /modified_by="User"
                     /label="traJ"
     CDS             complement(1871..2494)
                     /gene="catP"
     misc_feature    complement(2840..3459)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.84%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     CDS             complement(4236..5873)
                     /gene="repA"
     CDS             complement(6124..6660)
                     /gene="orfB"
     primer_bind     6847..6879
                     /Sequence="CACCTCCTTTTTGACTTTAAGCCTACGAATACC"
                     /Hairpin_Tm="None"
                     /Tm="65.6"
                     /Self_Dimer_Tm="None"
                     /%GC="42.4"
                     /created_by="primer3"
                     /label="NF-793"
     CDS             complement(7022..7645)
                     /gene="tetR"
     misc_signal     7667..7685
                     /gene="tetO"
     misc_feature    complement(7667..7672)
                     /gene="PtetR -10"
     misc_feature    complement(7690..7695)
                     /gene="PtetR -35"
     primer_bind     7718..7749
                     /Sequence="CTGGACTTCATGAAAAACTAAAAAAAATATTG"
                     /Hairpin_Tm="None"
                     /Tm="58.4"
                     /Self_Dimer_Tm="None"
                     /%GC="25.0"
                     /created_by="primer3"
                     /label="NF_1323"
     misc_feature    7747..7752
                     /gene="Pxyl -35"
                     /label="PsLucopt -35"
     misc_signal     7752..7770
                     /gene="tetO"
     misc_feature    7770..7775
                     /gene="Pxyl -10"
                     /label="PsLucopt -10"
ORIGIN      
        1 cctgcagtaa aggagaaaat tttatgagac caagtaaaaa attattaata gctataatat
       61 caatattctt aataagttca gttccagtaa gtgcacatgc agatagtgtt tttacacttg
      121 aagattttgt gggtgattgg agacaaactg ctggatataa tttagatcaa gttttagaac
      181 agggtggagt gagttctctt tttcaaaatc ttggtgtctc agttacacca atacaaagaa
      241 tagttttaag tggagaaaat ggattaaaga tcgatataca tgtaataatt ccttatgagg
      301 gactatcagg ggaccaaatg ggacaaattg agaaaatatt caaagttgta tatccagtag
      361 acgatcatca ctttaaagta atattacatt atggaacttt agtaattgat ggtgtaacac
      421 caaatatgat agattatttt ggtagaccat acgaagggat tgcagttttt gatggaaaga
      481 aaataaccgt aacaggcact ttgtggaatg gaaataaaat aatagatgaa aggttaatta
      541 atcctgatgg atctttattg tttagagtaa ctataaacgg tgttacaggt tggagacttt
      601 gtgaacgtat tctagcatag ggatcctata agttttaata aaactttaaa tagaaaaagg
      661 cttctctcat gagaagtctt ttttatttaa aataaatata aaataaaata gaggctataa
      721 atagcctcta ttttatgtga gaaatcccta aataaaaaga tgccagtgtg ctggaattcc
      781 ccttcaatat tccacaataa ggctgtaggc gctagggacc tctttagctc cttggaagct
      841 gtcagtagta ggggatcggt cttgccttgc tcgtcggtga tgtacttcac cagctccgcg
      901 aagtcgctct tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg caccccccgg
      961 ccgttttagc ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc ccatcatgac
     1021 cttgccaagc tcgtcctgct tctcttcgat cttcgccagc agggcgagga tcgtggcatc
     1081 accgaaccgc gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg
     1141 gcccaggtcg ccattgatgc gggccagctc gcggacgtgc tcatagtcca cgacgcccgt
     1201 gattttgtag ccctggccga cggccagcag gtaggccgac aggctcatgc cggccgccgc
     1261 cgccttttcc tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga taggtgggct
     1321 gcccttcctg gttggcttgg tttcatcagc catccgcttg ccctcatctg ttacgccggc
     1381 ggtagccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
     1441 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg
     1501 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg
     1561 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc aggaagggcg
     1621 aattctgcag atctgaccgg tctctgaaaa tataaaaacc acagattgat actaaaacct
     1681 tggttgtgtt gcttttcggg gcttaaatca aggaaaaatc cttgttttaa gcctttcaaa
     1741 aagaaacaca aggtctttgt actaacctgt ggttatgtat aaaattgtag attttagggt
     1801 aacaaaaaac accgtatttc tacgatgttt ttgcttaaat acttgttttt agttacagac
     1861 aaacctgaag ttaactattt atcaattcct gcaattcgtt tacaaaacgg caaatgtgaa
     1921 atccgtcaca tactgcgtga tgaacttgaa ttgccaaagg aagtataatt ttgttatctt
     1981 ctttataata tttccccata gtaaaaatag gaatcaaata atcatatcct ttctgcaaat
     2041 tcagattaaa gccatcgaag gttgaccacg gtatcataga tacattaaaa atgttttccg
     2101 gagcatttgg ctttccttcc attctatgat tgtttccata ccgttgcgta tcactttcat
     2161 aatctgctaa aaatgattta aagtcagact tacactcagt ccaaaggctg gaaaatgttt
     2221 cagtatcatt gtgaaatatt gtatagcttg gtatcatctc atcatatatc cccaattcac
     2281 catcttgatt gattgccgtc ctaaactctg aatggcggtt tacaatcatt gcaatataat
     2341 aaagcattgc aggatatagt ttcattccct tttcctttat ttgtgtgata tccactttaa
     2401 cggtcatgct gtaggtacaa ggtacacttg caaagtagtg gtcaaaatac tcttttctgt
     2461 tccaactatt tttatcaatt ttttcaaata ccatctaagt tccctctcaa attcaagttt
     2521 atcgctctaa tgaacaaaga tattatacca catttttgtg aatttttcaa cttgcccact
     2581 tcgactgcac tcccgactta ataacttctt gaacacttgc cgaaaaagaa aaactgccgg
     2641 gtacgtaccc gggatcgatc cccgccgagc gctcttccgc ttcctcgctc actgactcgc
     2701 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2761 tatccactga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     3061 gtaggtatca cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3481 agtggaacga aaactcacgt taagggattt tggtcatgaa atgcaagttt ctaactaaca
     3541 tacatcatag ttactaaact atggtaacta taattttatt aactatatta agcactgatt
     3601 agtactataa ctcaatataa gcatatcccc tgtatcgtaa ctagagaacc aaacgacgga
     3661 aaaagcgata tagataagtt tagatagtgc accaattttt tacttttcta ttttagaatc
     3721 tagcatttcc aatgcttact aatactattt ttgattttta acttgtttta aaaaagcacc
     3781 tactaattag gattctagct ctatattcta gttatacatt ctctatattc tagctctata
     3841 ttctagttat acattctcta tattctagct ctatattcta gttatacatt ctctatattc
     3901 tagctctata ttctagttat acattctcta tattctagct ctatattcta gttatacatt
     3961 ctctatattc tagctctata ttctagttat acattctcta tattctagct ctatattcta
     4021 gttatacatt ctctaaaaaa catatatata tatactacat atatatgtac aattatttta
     4081 ccaaaagaaa tttttaaaac ggcttaattt caatactttg atgccatttt ttttttgcaa
     4141 ttctcgtaca attattttac caaaagaaat ttttattaat tagttttttt gcataaaaat
     4201 aggactagaa aatcaattct aatcctatca aaaatctact tgtttaatct atatgtatag
     4261 tacataaatc ctctatcatt tattttagtt tcccatacat ctaaaatttt agtatctaat
     4321 aaagattcta tcatatttga aattctttgc tgtgttactc ctaaatcttt tgctaattcc
     4381 tcttgattaa ttcttaatat atttcctttt attttatcat ctttgaccaa taaatcttgc
     4441 ttatatctca tatgagtata taatcttaat tcttcagttg aaattattcc ccaaattact
     4501 gccatggtaa caaagtaact aatagtatat tgtttatcta tattacatct aacttctttt
     4561 acattgtatg tatcttttat tcctagcttt gtaacacctt ttgtttttgt aatataatca
     4621 ttatgttgaa gttcttttaa tgtttctctt aatgtctttt cactcataac aatgttttta
     4681 actttcttct tacgtttata ggttatcagc tccattatat cgtctatgtt taattctcta
     4741 tctttattgt taagtaacac attatagata aacaattgat taccagtcat tattttaacc
     4801 cctttcctat tcttatattt caaatccttt gagtgcttat gtggcatatt gaacaaagta
     4861 tcttcatcac tatatataaa atctgattct attttattcc aacaatcact attaaactca
     4921 caacctgtac aaccaaaatt aaccgtttta agcttctcat acatatactt gacttgatat
     4981 tctagctcac tatttgaaag actattttgt gatatatagt tccattctct agcaacggat
     5041 attacttgac ttaaactttt attcctcaat cttaaagtca caactatttt ttgcaaatct
     5101 aggtttttat gtccatcttt actaccattt tttaaaatat ctactataca atttggtagc
     5161 ttactatcta atatatattt tgtattagta ttacctactg tttttacatc tttgcaatat
     5221 tttttagcta acttttctat gtcatatcta tatatatttt tgtcttggtg tattattttt
     5281 acttgtttag tagtattttt aatattatat gtataaggta ctcttaatac ttgagtagat
     5341 aaatttgctt gtttatctgc acccaattta tttattaatg tatttgtaac ttctgatact
     5401 aacttaatgt tattagtttt attaattagc acataaacat ggaatccatt tccactatca
     5461 actaggatat gataatgtaa tcctatctta gtaaataaat taattatatc tttataatta
     5521 aaaccttgtc ctaattcttt tttgtcaaaa tcaaatccta atgcatatct atttattaag
     5581 ttttcattgg ttcctgcttt tccatcagtt gtacataggt taaaaaatgt attttcataa
     5641 taaatatttt ttgcgaaggt aaattctact atatcatcta tatttttaaa aaaagttgtt
     5701 ttactatatt ctttaaattc ttctccgagt tctaattgtt tactattact tttattattt
     5761 tgaaatactc taacatattc attatcaata agattatttc cttgtcctat accatctcta
     5821 aatactgcca ttagaaattt tttcaactta tattttgaat ctaattgttc catatattta
     5881 ttatctcctt atctagtttt tttaagagat aacactacat taaatatata cttattgact
     5941 aatcaacttt tacaactatt gaataatttt agcttagtat gtataataat attgttaaga
     6001 tgctaaaatt gatagcaata ttattatata actacagata cttaatcagt tagaccgata
     6061 ttggagttgg tagctccata aactcaattg gtagttgagt tgtcattgtc tagtagtatt
     6121 atctcatttt gttgttatct taaaatatta ttttaaaaat actctgcttg gtcggcttag
     6181 ggtatttttt ttgtttaatt ccaatattgg ttcaaactcc tgtgtaatac ttttgacact
     6241 tccatctgta ttgaattgac agttgatatg gtatctcatt attggcttgt tcttatgttt
     6301 ttctatgtat ttatttttta ctttcaaata atttgtattt ccatttttag ttatgtttaa
     6361 cacactatcc ttattactca ataaaattat tttttgtttt aacatatgat ttatagaatc
     6421 taaaaattga tattgactta taatagtact ttctacctct ctagcttttt ctagtgtttc
     6481 aaattcttca aactctgcta tattgctata gtatagctta tcattaataa tattatctga
     6541 caactttaat tccattttat aaaacaattc caagttatgt ataaaatttt taatgttaca
     6601 acatatagac ttattatttg ttcttatatg gtatctccat ttactacttc tagctctcat
     6661 gttatatgca caactactcc caacataaac atatattgct tgttctaact catatttttg
     6721 taaaatttca acatcatatc cagtcaaatc gtactctctc ttatatacaa atggtttttc
     6781 taattttttg ttcaataaat ctcctcctat ttcttactta atttagctaa aaatctattt
     6841 atatttcacc tcctttttga ctttaagcct acgaatacca tatttatgat tataacatct
     6901 tctatttttc ccaaatcctt acatctcccc taatttttag acttaagggc gaattctgca
     6961 tcaagctagc ataaaaataa gaagcctgca tttgcaggct tcttattttt atggtacctt
     7021 aagacccact ttcacattta agttgttttt ctaatccgca tatgatcaat tcaaggccga
     7081 ataagaaggc tggctctgca ccttggtgat caaataattc gatagcttgt cgtaataatg
     7141 gcggcatact atcagtagta ggtgtttccc tttcttcttt agcgacttga tgctcttgat
     7201 cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat aatgcattct
     7261 ctagtgaaaa accttgttgg cataaaaagg ctaattgatt ttcgagagtt tcatactgtt
     7321 tttctgtagg ccgtgtacct aaatgtactt ttgctccatc gcgatgactt agtaaagcac
     7381 atctaaaact tttagcgtta ttacgtaaaa aatcttgcca gctttcccct tctaaagggc
     7441 aaaagtgagt atggtgccta tctaacatct caatggctaa ggcgtcgagc aaagcccgct
     7501 tattttttac atgccaatac aatgtaggct gctctacacc tagcttctgg gcgagtttac
     7561 gggttgttaa accttcgatt ccgacctcat taagcagctc taatgcgctg ttaatcactt
     7621 tacttttatc taatctagac atcattaatt cctccttttt gttgacatta tatcattgat
     7681 agagttattt gtcaaactag ttttttattt cgatgccctg gacttcatga aaaactaaaa
     7741 aaaatattga cactctatca ttgatagagt ataattaaaa taagcttgat cgtagcgtta
     7801 acagatctga gct
//
