LOCUS       286_TagRFPT_C1_S       10504 bp ds-DNA     circular     01-OCT-2015
DEFINITION  Cloning vector pUC19, complete sequence.
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19 other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     To prepare vector for cloning: 
COMMENT     -Digest with ClaI and NgoMIV to fuse 5' homology arm directly to
            mKate2, or 
COMMENT     -Digest with AvrII and NgoMIV to include a flexible linker (for
            C-terminal tags).
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    398..1010
                     /label=This sequence will be replaced by the homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7661..8267
                     /label=This sequence will be replaced by the homology
                     arm(1)
                     /ApEinfo_label=This sequence will be replaced by the
                     homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      complement(8332..8337)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
                     /label=Plac promoter (drives lacZalpha; counter-clockwise
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(2786..2926,2974..3807)
                     /label=SQT-1
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6116..7141
                     /note="hygromycinR"
                     /label=hygR
                     /ApEinfo_fwdcolor=#006666
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      complement(8356..8361)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
                     /label=Plac promoter (drives lacZalpha;
                     counter-clockwise(1)
                     /ApEinfo_label=Plac promoter (drives lacZalpha;
                     counter-clockwise
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(join(4180..4421,4473..4843,4895..5310))
                     /label=Cre
                     /ApEinfo_fwdcolor=#6699cc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    complement(8381..8393)
                     /note="CAP protein binding site"
                     /bound_moiety="catabolite activator protein (CAP)"
                     /label=CAP protein binding site
                     /ApEinfo_fwdcolor=#990099
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        complement(8685..9237)
                     /note="RNAII transcript (complementary strand)"
                     /label=RNAII transcript (complementary strand)
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8685..9273)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /label=pMB1 origin of replication (counter-clockwise) (RN
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        9127..9234
                     /note="RNAI transcript"
                     /label=RNAI transcript
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1995..2000
                     /label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8667..9349)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      complement(9247..9252)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /label=RNAII promoter (counter-clockwise); CGTAAT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7559..7570
                     /label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9447..10106)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      9091..9096
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /label=RNAI promoter (clockwise); TTGAAG
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7631..7660
                     /label=Myc Tag
                     /ApEinfo_fwdcolor=#cc33cc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      9113..9118
                     /note="RNAI promoter (clockwise); GCTACA"
                     /label=RNAI promoter (clockwise); GCTACA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     5'UTR           join(2700..2727,2784..2785)
                     /label=sqt-1 5'UTR
                     /ApEinfo_fwdcolor=#ccffcc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9444..10304)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1950..1960
                     /label=intron(1)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      complement(10348..10353)
                     /note="bla promoter (counter-clockwise); GAGACA"
                     /label=bla promoter (counter-clockwise); GAGACA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7571..7600
                     /label=Myc Tag(1)
                     /ApEinfo_label=Myc Tag
                     /ApEinfo_fwdcolor=#ff99ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(1038..1181,1233..1460,1512..1694,1746..1922)
                     /label=TagRFP-T
                     /ApEinfo_fwdcolor=#ff3300
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    complement(8305..8327)
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 3037..3037
                     /label=e1350
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2001..2003
                     /label=spacer(1)
                     /ApEinfo_label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    661..966
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(7705..8010)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB(1)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      complement(9268..9273)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /label=RNAII promoter (counter-clockwise); TTGAGA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(join(146..395,8279..8287))
                     /label=LacZalpha
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           3808..3844
                     /label=sqt-1 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2034..2415
                     /label=let-858 Terminator
                     /ApEinfo_fwdcolor=#cc0000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(8279..8299)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1923..1943
                     /label=TEV site
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7601..7630
                     /label=Myc Tag(2)
                     /ApEinfo_label=Myc Tag
                     /ApEinfo_fwdcolor=#cc66cc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      complement(10369..10374)
                     /note="bla promoter (counter-clockwise); TTCAAA"
                     /label=bla promoter (counter-clockwise); TTCAAA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             754..963
                     /label=ccdB(2)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(7708..7917)
                     /label=ccdB(3)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(5311..5710)
                     /label=hsp16-41 promoter
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           7167..7518
                     /label=unc-54 3'UTR
                     /ApEinfo_fwdcolor=#990000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           complement(3845..4176)
                     /label=tbb-2 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#993333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2416..2785
                     /label=Psqt1
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          7553..7558
                     /label=intron(2)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7519..7552
                     /label=lox2272
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1961..1994
                     /label=lox2272(1)
                     /ApEinfo_label=lox2272
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    2004..2030
                     /label=HA
                     /ApEinfo_fwdcolor=#6600ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        5711..6073
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;
                     /ApEinfo_revcolor=pink; /ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
                     /label=Prps-0
                     /ApEinfo_fwdcolor=#339933
                     /ApEinfo_revcolor=#339933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_structure  1011..1037
                     /label=Flexible Linker (will be deleted if ClaI is used)
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8268..8274
                     /label=This sequence will be deleted if ClaI is used
                     /ApEinfo_fwdcolor=#ffcc99
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     378..395
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtTA CCCGAAGTAT
      421 GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT
      481 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTTC GGTAAGCACA ACCATGCAGA
      541 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG
      601 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCCGA CGAGAACAGG GGCTGGTGAA
      661 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA
      721 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT
      781 CTGCTGTCAG ATAAAGTCCC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC
      841 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG
      901 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA
      961 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGAcctag GGAGCATCGG
     1021 GAGCCTCAGG AGCATCGATG GTCTCCAAGG GAGAGGAGCT CATCAAGGAG AACATGCACA
     1081 TGAAGCTCTA CATGGAGGGA ACCGTCAACA ACCACCACTT CAAGTGCACC TCCGAGGGAG
     1141 AGGGAAAGCC ATACGAGGGA ACCCAAACCA TGCGTATCAA Ggtaagttta aacatatata
     1201 tactaactaa ccctgattat ttaaattttc agGTCGTCGA GGGAGGACCA CTCCCATTCG
     1261 CCTTCGACAT CCTCGCCACC TCCTTCATGT ACGGATCCCG TACCTTCATC AACCACACCC
     1321 AAGGAATCCC AGACTTCTTC AAGCAATCCT TCCCAGAGGG ATTCACCTGG GAGCGTGTCA
     1381 CCACCTACGA GGACGGAGGA GTCCTCACCG CCACCCAAGA CACCTCCCTC CAAGACGGAT
     1441 GCCTCATCTA CAACGTCAAG gtaagtttaa acagttcggt actaactaac catacatatt
     1501 taaattttca gATCCGTGGA GTCAACTTCC CATCCAACGG ACCAGTCATG CAAAAGAAGA
     1561 CCCTCGGATG GGAGGCCAAC ACCGAGATGC TCTACCCAGC CGACGGAGGA CTCGAGGGAC
     1621 GTACCGACAT GGCCCTCAAG CTCGTCGGAG GAGGACACCT CATCTGCAAC TTCAAGACCA
     1681 CCTACCGTTC CAAGgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1741 ttcagAAGCC AGCCAAGAAC CTCAAGATGC CAGGAGTCTA CTACGTCGAC CACCGTCTCG
     1801 AGCGTATCAA GGAGGCCGAC AAGGAGACCT ACGTCGAGCA ACACGAGGTC GCCGTCGCCC
     1861 GTTACTGCGA CCTCCCATCC AAGCTCGGAC ACAAGCTCAA CGGAATGGAC GAGCTCTACA
     1921 AGGAGAATCT GTACTTTCAA TCCGGAAAGg taagtttaaa ATAACTTCGT ATAGGATACT
     1981 TTATACGAAG TTATtttcag GGATACCCAT ACGACGTCCC AGACTACGCC TAAGGATGAT
     2041 CGACGCCaAC GTCGTTGAAT TTTCAAATTT TAAATACTGA ATATTTGTTT TTTTTCCTAT
     2101 TATTTATTTA TTCTCTTTGT GTTTTTTTTC TTGCTTTCTA AAAAATTAAT TCAATCCAAA
     2161 TCTAAacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa
     2221 cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg
     2281 gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa
     2341 caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg
     2401 aggaaggaaa actagctgcc ctcgattgtc tatcttccat tctcaccaag tttcaacaac
     2461 tttattcagt cattcttctc tggttcattt cccaaccaat cttcccgaat aaagcccatc
     2521 tgtcctttcg gccacattcc tcacctacat ccgtactcct tatctcccgt tcttatccag
     2581 tcttttatcg tctctgcgtc tcgcacactg tctcgtcggt aatgtaaaaa aaaaacaaaa
     2641 aaaacttgaa aacttgcgga aagatttcct aataaaagga gacgaatccg gtaggaaatc
     2701 gacaagtctc tgcttctcga agactaggtg agttacattt ctttggccga attattttga
     2761 aatcctaaat ttgataattt cagagATGTC TGTAAAACTT GCGTGTTATG TGACGGCTTC
     2821 GGTCACTGTC GCCACTCTTA TGGTATGCTT CATGACCATG TCAACCATCT ACTCGGAGGT
     2881 TGATGGATTC AGAGAGAAGC TTGATACCGA GATGAATGTG TTCAGAgtga gttaaattat
     2941 attttgaatt ttaaataatt tttaattttc tagCAATCTA CCAATGGATT GTGGAAGGAC
     3001 ATAGTTGTCA TCGGAAGATC TAGCAAGCGT GTCCGTTGTC AATATGAAGA GACCAACGCT
     3061 ACCCCAACTC CACATGCTGA TGGATCCCCA TCTGCTCCAC CAGGTCAACC ACCAGCAGTT
     3121 CCACCAGTCT TCAACCAGCC AAAGACTCCA AATGGAGCCA ATGGAAATGG ACCAACCTGC
     3181 AACTGCAATG CTGATAACAA GTGCCCAGCT GGACCATCCG GACCAAAGGG AGTTCCAGGA
     3241 GTTCCAGGAC TCGACGGAGT TCCAGGACTT GACGGTGTTC CAGGAGTTGG AGCTGATGAT
     3301 ATCGCTCCAC AACGCGAGTC TGTCGGATGC TTCACTTGCC CACAAGGACC AGTTGGACCA
     3361 CCAGGAGCTC TTGGAAGACC AGGACCACGT GGACTTCCAG GACCAAGAGG ACAAAATGGA
     3421 AACCCAGGAA GAGATGGACA ACCAGGACAT CCAGGAGAGC AAGGATCATC CGGTCAAATC
     3481 GGAAAGATCG GAGAGCCAGG ACCACCAGGA GAGAAGGGAC GCGACGCCGA GCATCCAATC
     3541 GGAAGACCAG GACCAAAGGG ACCAAGAGGA GATCAAGGAC CAACAGGACC AGCTGGACAG
     3601 AACGGTCTTC ACGGACCACC AGGAGAGCCA GGAACCGTTG GACCAGAAGG ACCATCTGGA
     3661 AAGCAAGGAC GTCAAGGACC AGACGGAACC CAGGGAGAGA CTGGACCAGA CGGAAGACCA
     3721 GGAAAGGATG CCGAGTACTG CCAGTGCCCA GACAAGTCTC CACCATCAGA GGCTGTCAAC
     3781 GCCAACCGTG GATACAGAAA TATCTAAatt gttggtgttt tctaataaaa tatttgagat
     3841 tcagtgagac ttttttcttg gcggcacaat aaagtttcct ctttttgttg ggtcactcaa
     3901 cgaacacaat acaaatattt gagcgctcgc atttttctca acctttcggg tttaaagcac
     3961 ttcaaaatgg aattgaagag agattgtgaa agtacatcag acagaatcac attttgatag
     4021 cattcacttc actcagatgc aaacttttca gtgactataa aaagtcataa aataataaca
     4081 ttaaaaaagc aaataaatta gcgagagaat tttttgacaa aaagaaagaa gagtgataga
     4141 gaagaaggga atgcttgaaa ggattttgca tttatcttaA TCACCGTCTT CGAGAAGACG
     4201 AACCATGGCG CCAGTTTCAG AATCCAAGTT TCTAATATAG TTCATCACAA TGTTGACGTT
     4261 GGTCCAACCT CCGGCTTGCA TAATCTCTGG GATGGAAACT CCAGCGCGAG CCATGTCACG
     4321 GGCAGCTCCG ACACGGGCGG AATGTCCAGA CCAAGCCAAG TAACGTTGTC CGGAATCATC
     4381 CTTGGCTCCG TAGATGAGAC GGTGGGTAGC TTCGAAGATT Cctgaaaatt tattttcagc
     4441 ttattcttag ctaatatgtt gtttaaactt acCTTCAAGA GCTCGAGTCG AGAGTTGTGA
     4501 TGTTGCACTC GGGGCAGCAA CTCCGTTCTT GCGAACACGA CAGAACAGGT AGTTGTTTGG
     4561 ATCATCGGCG ACTCCGGAAA CCGAAATCCA GCGCTCAACG AGCTTGGTAA CTCCAAGGGA
     4621 GAGCGCTTTT TCGACTCCGG CAGTCGAGAC CAGCGTTTTT GTACGTCCGA TGTGGATGAG
     4681 CATTCTTCCT CCGTCTGTGC GACTAATATC CTTGACGCGA ATGCGGGCAA TTTCGGCGAT
     4741 ACGGAGAAGT GTGTTGTAAG CAATTCCGAG GAAGGCGAGG TTTCGGATGT CCTGGCAACG
     4801 ATCGGAATTT TCCATAAGAG AACGGACCTG ATCGAAGTCG GTTctgaaaa tttcgtcaag
     4861 tcttaggatt gaatacatta atatttaaac ttacCTTTCG AAGGCGAGGG CTTGCTTGGC
     4921 TCTCTCTCCA GCATCAACAT TTTCCTTGCG GATACGTCTC ATTACGAGAG ATACGGCATT
     4981 AGAATCTGAT GGGCGTGGAA GTCCAGATCG GCGATGGAGC ATATTGAGCT GTCCGAGATG
     5041 TTGTTGGATC GTCTTCACCG CCAATCCACG AGCTTGGAGG TAGAGCAAGT AGTCACGAAC
     5101 GTCTTCTGGC TCGGCTGGGA ACCACTTGCG GTTGTTAAGC TTGCACCAGG CAGCCCAAGA
     5161 ACGGCACACT GAGAGAAGCA TCTTCCAGGT GTGCTCGGAG AAGGCTTGTC TGTCACGGAA
     5221 CATATCCATG AGGTTCTTGC GAACTTCATC ACTTGTCGCG TCAACTGGAA GTGCTGGAAG
     5281 ATTTTGATGA ACAGTGAGAA GATTTGACAT attttcgaag ttttttagat gcactagaac
     5341 aaagcgtgtt ggcttcctct gagcccgctt tccttatata cccgcattct gcagccttac
     5401 agaatgttct agaaggtcct agatgcattc gtttgaaaat actcccggtg ggtgcaaaga
     5461 gacgcagacg gaaaatgtat ctgggtctct ttattgtgta cactactttt ccatgtaccg
     5521 aatgtgagtc gccctccttt tgcaacaagc agctcgaatg ttctagaaaa aggtggaaaa
     5581 tagtataaat accgttgaaa ataaataccg aacaacattt gctctaattg tgaaattaga
     5641 aatcttcaaa ctataatcat gtcactttac cactatttcc gtccagctca acgttccgtt
     5701 tttggtgatc ATTTTTGCTT TCGTCGTAAA TCTACACACG CGTCTCTTCC GTGCGAGAGT
     5761 CCAAGCCAGC AGCCAAATTC GTTGACTGAG TATTCAACGT TTATACGTTG TCGGCAACGA
     5821 GAAATAGGAA AATGCATCGG GAAATGTTCT TTTTTCGATT TTTTCCAAGG TTTTGACAAA
     5881 TTTTACCACG AATTTTGCTA TGTTTTCAAT TAAAAAATAT GTTATTCAAC TGTTTCTATG
     5941 AGGAAAATAA GGCTTTGCAT GTAATTTTCT TATTCAGCAT AATTTTTAAT TAATTTGAAT
     6001 TTTCTGTCCT AACGTTTATT TTGTTTTCTT GGTTATGACT GATCTGAAAT TAATTTTTGA
     6061 ATTTTAAGGT AATATGTCAG GCGGTGCCGC AAGTTTGTAC AAAAAAGCAG GCTCCATGAA
     6121 AAAGCCTGAA CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT
     6181 CTCCGACCTG ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG
     6241 AGGGCGTGGA TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA
     6301 TGTTTATCGG CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA
     6361 ATTCAGCGAG AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA
     6421 CCTGCCTGAA ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT
     6481 CGCTGCGGCC GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG
     6541 TCAATACACT ACATGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG
     6601 GCAAACTGTG ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT
     6661 GCTTTGGGCC GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA
     6721 CAATGTCCTG ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT
     6781 CGGGGATTCC CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT
     6841 GGAGCAGCAG ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCGCGGCT
     6901 CCGGGCGTAT ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG TTGACGGCAA
     6961 TTTCGATGAT GCAGCTTGGG CGCAGGGTCG ATGCGACGCA ATCGTCCGAT CCGGAGCCGG
     7021 GACTGTCGGG CGTACACAAA TCGCCCGCAG AAGCGCGGCC GTCTGGACCG ATGGCTGTGT
     7081 AGAAGTACTC GCCGATAGTG GAAACCGACG CCCCAGCACT CGTCCGAGGG CAAAGGAATA
     7141 GACCCAGCTT TCTTGTACAA AGTGGGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT
     7201 CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG
     7261 TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT
     7321 TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA
     7381 TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT
     7441 TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT
     7501 TTATGACCGC AATTTTTAAT AACTTCGTAT AGGATACTTT ATACGAAGTT ATtttcagGG
     7561 AGCCGGATCT GAACAAAAAC TTATTTCTGA AGAGGATCTT GAGCAAAAGC TCATCTCCGA
     7621 GGAGGACCTC GAGCAGAAGT TGATCAGCGA GGAAGACTTG ccggcTCGAC CTGCAGACTG
     7681 GCTGTGTATA AGGGAGCCTG ACATTTATAT TCCCCAGAAC ATCAGGTTAA TGGCGTTTTT
     7741 GATGTCATTT TCGCGGTGGC TGAGATCAGC CACTTCTTCC CCGATAACGG AGACCGGCAC
     7801 ACTGGCCATA TCGGTGGTCA TCATGCGCCA GCTTTCATCC CCGATATGCA CCACCGGGTA
     7861 AAGTTCACGG GGGACTTTAT CTGACAGCAG ACGTGCACTG GCCAGGGGGA TCACCATCCG
     7921 TCGCCCGGGC GTGTCAATAA TATCACTCTG TACATCCACA AACAGACGAT AACGGCTCTC
     7981 TCTTTTATAG GTGTAAACCT TAAACTGCAT TTCACCAGCC CCTGTTCTCG TCGGCAAAAG
     8041 AGCCGTTCAT TTCAATAAAC CGGGCGACCT CAGCCATCCC TTCCTGATTT TCCGCTTTCC
     8101 AGCGTTCGGC ACGCAGACGA CGGGCTTCAT TCTGCATGGT TGTGCTTACC GAACCGGAGA
     8161 TATTGACATC ATATATGCCT TGAGCAACTG ATAGCTGTCG CTGTCAACTG TCACTGTAAT
     8221 ACGCTGCTTC ATAGCATACC TCTTTTTGAC ATACTTCGGG Ttcctaggaa atcgataaca
     8281 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9181 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10441 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    10501 cgtc     
//
