LOCUS MSP2253 7092 bp DNA circular UNA 21-OCT-2015 DEFINITION pACYCDUET-1. ACCESSION urn.local...c-5b1hjko VERSION urn.local...c-5b1hjko KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers protein_bind 5..25 /vntifkey=31 /label="lacO" RBS 58..63 /vntifkey=32 /label="RBS-1" CDS 71..3331 /created_by="User" /modified_by="User" /label="HumanSaCas9-NLS-3xFLAG" promoter 3401..3417 /vntifkey=30 /modified_by="User" /label="T7 promoter" unsure 3418..3438 /Color="#ff7400" /created_by="User" /label="Site 1" misc_RNA 3439..3522 /created_by="User" /modified_by="User" /label="Sa-sgRNA(84)" terminator 3545..3592 /vntifkey=43 /modified_by="User" /label="T7 terminator" CDS complement(3813..4472) /vntifkey=4 /label="CAT" rep_origin 4834..5746 /vntifkey=33 /modified_by="User" /label="P15A origin" CDS complement(5870..6952) /vntifkey=4 /note="lacI gene" /label="lacI" promoter 7076..7092 /vntifkey=30 /modified_by="User" /label="T7 promoter" ORIGIN 1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 61 gagatatacc atgggcaaac ggaactacat cctggggctt gacattggga taaccagcgt 121 tggctacgga attattgatt atgagacacg cgatgtgatt gacgccgggg ttaggctgtt 181 caaagaggcc aacgttgaaa acaacgaggg aagacggagt aagcgcggag caagaagact 241 caagcgcaga cggagacatc ggattcagag ggtgaaaaag ctgctcttcg attacaatct 301 cctgaccgat catagtgagc tgagcggaat caacccctac gaggcgcgag tgaaagggct 361 ttcccagaag ctgtccgaag aggagttctc cgccgcgttg ctgcacctgg ccaaacggag 421 gggggttcac aatgtaaacg aagtggagga ggacacgggc aatgaactta gtacgaaaga 481 acagatcagt aggaactcta aggctctcga agagaaatac gtcgctgagt tgcagcttga 541 gagactgaaa aaagacggcg aagtacgcgg atctattaat aggttcaaga cttcagatta 601 cgtaaaggaa gccaagcagc tcctgaaagt acagaaagcg taccatcagc tcgatcagag 661 cttcatcgat acctacatag atttgctgga gacacggagg acatactacg agggcccagg 721 ggaaggatct ccttttgggt ggaaggacat caaggaatgg tacgagatgc ttatgggaca 781 ttgtacatat tttccggagg agctcaggag cgtcaagtac gcctacaatg ccgacctgta 841 caatgccctc aatgacctca ataacctcgt gattaccagg gacgagaacg agaagctgga 901 gtactatgaa aagttccaga ttatcgagaa tgtgtttaag cagaagaaga agccgacact 961 taagcagatt gcaaaggaaa tcctcgtgaa tgaggaagat atcaagggat acagagtgac 1021 aagtacaggc aagcccgagt tcacaaatct gaaggtgtac cacgatatta aggacataac 1081 cgcacgaaag gagataatcg aaaacgctga gctcctcgat cagatcgcaa aaattcttac 1141 catctaccag tctagtgagg acattcagga ggaactgact aatctgaaca gtgagctcac 1201 ccaagaggaa attgagcaga tttcaaacct gaaaggctac accgggacgc acaatctgag 1261 cctcaaagca atcaacctca ttctggatga actttggcac acaaatgaca accaaattgc 1321 catattcaac cgcctgaaac tggtgccaaa aaaagtggat ctgtcacagc aaaaggaaat 1381 ccctacaacc ttggttgacg attttattct gtcccccgtt gtcaagcgga gcttcatcca 1441 gtcaatcaag gtgatcaatg ccatcattaa aaaatacgga ttgccaaacg atataattat 1501 cgagcttgca cgagagaaga actcaaagga cgcccagaag atgattaacg aaatgcagaa 1561 gcgcaaccgc cagacaaacg aacgcataga ggaaattata agaacaaccg gcaaagagaa 1621 tgccaagtat ctgatcgaga aaatcaagct gcacgacatg caagaaggca agtgcctgta 1681 ctctctggaa gctatcccac tcgaagatct gctgaataat ccattcaatt acgaggtgga 1741 ccacatcatc cctagatccg taagctttga caattccttc aataacaaag ttctggttaa 1801 acaggaggaa aattctaaaa aagggaaccg gaccccgttc cagtacctga gctccagtga 1861 cagcaagatt agctacgaga cttttaagaa acatattctg aatctggcca aaggcaaagg 1921 caggatcagc aagaccaaga aggagtacct cctcgaagaa cgcgacatta acagatttag 1981 tgtgcagaaa gatttcatca accgaaacct tgtcgatact cggtacgcca cgagaggcct 2041 gatgaatctc ctcaggagct acttccgcgt caataatctg gacgttaaag tcaagagcat 2101 aaatggggga ttcaccagct ttctgaggag aaagtggaag tttaagaagg aacgaaacaa 2161 aggatacaag caccatgctg aggatgcttt gatcatcgct aacgcggact ttatctttaa 2221 ggaatggaaa aagctggata aggcaaagaa agtgatggaa aaccagatgt tcgaggagaa 2281 gcaggcagag tcaatgcctg agatcgagac agagcaggaa tacaaggaaa ttttcatcac 2341 ccctcatcag attaaacaca taaaggactt caaagactat aaatactctc atagggtgga 2401 caaaaaaccc aatcgcaagc tcattaatga caccctgtac tcaacacgga aggatgataa 2461 aggtaatacc ttgattgtga ataatcttaa tggattgtat gacaaagata acgacaagct 2521 caagaagctg atcaacaagt ctccagagaa gctccttatg tatcaccacg acccacagac 2581 ttatcagaaa ttgaaactga tcatggagca atacggggat gagaagaacc cactctacaa 2641 atattatgag gaaacaggta attacctgac caagtactcc aagaaggata acggaccagt 2701 gatcaaaaag ataaagtact atggcaacaa acttaatgcg catttggaca taactgacga 2761 ttaccccaat tctcgaaaca aggttgtgaa gctctccctg aagccttata gatttgacgt 2821 gtacctggat aatggggttt ataaattcgt caccgtgaaa aatctggacg tgatcaaaaa 2881 ggagaactat tatgaagtaa actcaaagtg ctatgaggag gcgaagaagc tgaagaagat 2941 ctccaatcag gccgagttca tcgcttcctt ctataagaac gatctcatca agatcaatgg 3001 agagctttat cgcgtcattg gtgtgaacaa tgacttgctg aacaggatcg aagtcaatat 3061 gatagacatt acctaccggg agtatctcga aaacatgaat gataaacggc cgcctcacat 3121 catcaagaca atcgcatcta aaactcagtc aataaaaaag tactctaccg atatcctggg 3181 gaatctctat gaagtgaagt caaagaagca cccacaaatc attaaaaaag gtggatcccc 3241 caagaagaag aggaaagtct cgagcgacta caaagaccat gacggtgatt ataaagatca 3301 tgacatcgat tacaaggatg acgatgacaa gtaaagcggc cgcataatgc ttaagtcgaa 3361 cagaaagtaa tcgtattgta cacggccgca taatcgaaat taatacgact cactataggg 3421 gcacgggcag cttgccgggt tttagtactc tgtaatgaaa attacagaat ctactaaaac 3481 aaggcaaaat gccgtgttta tctcgtcaac ttgttggcga gatttttttt ccgctgagca 3541 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaacc 3601 tcaggcattt gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca 3661 gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt 3721 gctttcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta gcaaccaggc 3781 gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag 3841 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga 3901 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatagtg 3961 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc 4021 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc 4081 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg 4141 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa 4201 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaactcc 4261 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta 4321 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta 4381 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca 4441 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc 4501 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct 4561 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca 4621 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg 4681 gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg 4741 aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt 4801 ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag cggagtgtat actggcttac 4861 tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg 4921 caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact 4981 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt 5041 tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt 5101 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg 5161 gcgaaacccg acaggactat aaagatacca ggcgtttccc ctggcggctc cctcgtgcgc 5221 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct 5281 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca 5341 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 5401 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt 5461 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg 5521 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac 5581 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat 5641 ctcaagaaga tcatcttatt aatcagataa aatatttcta gatttcagtg caatttatct 5701 cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc tcatgttagt 5761 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg 5821 agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc 5881 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 5941 aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca 6001 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt 6061 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt 6121 cttcggtatc gtcgtatccc actaccgaga tgtccgcacc aacgcgcagc ccggactcgg 6181 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa 6241 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc 6301 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca 6361 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac 6421 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac 6481 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag 6541 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc 6601 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca 6661 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt 6721 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc 6781 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca 6841 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct 6901 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca 6961 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt 7021 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaaattaata 7081 cgactcacta ta //