LOCUS       MSP1830                 8097 bp    DNA     circular SYN 21-OCT-2015
DEFINITION  pCAG-CFP.
ACCESSION   urn.local...b-5b1hjkl
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
            .
FEATURES             Location/Qualifiers
     promoter        18..1718
                     /created_by="User"
                     /label="CAG promoter"
     CDS             1769..5029
                     /created_by="User"
                     /modified_by="User"
                     /label="hSaCas9 (KKH variant)"
     terminator      5051..5554
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /modified_by="User"
                     /label="rb glob PA terminator"
     promoter        complement(5649..5678)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /modified_by="User"
                     /label="lac promoter"
     promoter        5784..5963
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /modified_by="User"
                     /label="SV40 promoter"
     unsure          5819..5896
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /Color="#aa00aa"
                     /modified_by="User"
                     /label="SV40 origin"
     terminator      5977..6108
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /modified_by="User"
                     /label="SV40 PA terminator"
     rep_origin      complement(6332..6951)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /modified_by="User"
                     /label="pBR322 origin"
     CDS             complement(7106..7966)
                     /gene="Ampicillin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /modified_by="User"
                     /label="AmpR"
     promoter        complement(8008..8036)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /modified_by="User"
                     /label="AmpR promoter"
ORIGIN      
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat ggggaaacgg aactacatcc tggggcttga
     1801 cattgggata accagcgttg gctacggaat tattgattat gagacacgcg atgtgattga
     1861 cgccggggtt aggctgttca aagaggccaa cgttgaaaac aacgagggaa gacggagtaa
     1921 gcgcggagca agaagactca agcgcagacg gagacatcgg attcagaggg tgaaaaagct
     1981 gctcttcgat tacaatctcc tgaccgatca tagtgagctg agcggaatca acccctacga
     2041 ggcgcgagtg aaagggcttt cccagaagct gtccgaagag gagttctccg ccgcgttgct
     2101 gcacctggcc aaacggaggg gggttcacaa tgtaaacgaa gtggaggagg acacgggcaa
     2161 tgaacttagt acgaaagaac agatcagtag gaactctaag gctctcgaag agaaatacgt
     2221 cgctgagttg cagcttgaga gactgaaaaa agacggcgaa gtacgcggat ctattaatag
     2281 gttcaagact tcagattacg taaaggaagc caagcagctc ctgaaagtac agaaagcgta
     2341 ccatcagctc gatcagagct tcatcgatac ctacatagat ttgctggaga cacggaggac
     2401 atactacgag ggcccagggg aaggatctcc ttttgggtgg aaggacatca aggaatggta
     2461 cgagatgctt atgggacatt gtacatattt tccggaggag ctcaggagcg tcaagtacgc
     2521 ctacaatgcc gacctgtaca atgccctcaa tgacctcaat aacctcgtga ttaccaggga
     2581 cgagaacgag aagctggagt actatgaaaa gttccagatt atcgagaatg tgtttaagca
     2641 gaagaagaag ccgacactta agcagattgc aaaggaaatc ctcgtgaatg aggaagatat
     2701 caagggatac agagtgacaa gtacaggcaa gcccgagttc acaaatctga aggtgtacca
     2761 cgatattaag gacataaccg cacgaaagga gataatcgaa aacgctgagc tcctcgatca
     2821 gatcgcaaaa attcttacca tctaccagtc tagtgaggac attcaggagg aactgactaa
     2881 tctgaacagt gagctcaccc aagaggaaat tgagcagatt tcaaacctga aaggctacac
     2941 cgggacgcac aatctgagcc tcaaagcaat caacctcatt ctggatgaac tttggcacac
     3001 aaatgacaac caaattgcca tattcaaccg cctgaaactg gtgccaaaaa aagtggatct
     3061 gtcacagcaa aaggaaatcc ctacaacctt ggttgacgat tttattctgt cccccgttgt
     3121 caagcggagc ttcatccagt caatcaaggt gatcaatgcc atcattaaaa aatacggatt
     3181 gccaaacgat ataattatcg agcttgcacg agagaagaac tcaaaggacg cccagaagat
     3241 gattaacgaa atgcagaagc gcaaccgcca gacaaacgaa cgcatagagg aaattataag
     3301 aacaaccggc aaagagaatg ccaagtatct gatcgagaaa atcaagctgc acgacatgca
     3361 agaaggcaag tgcctgtact ctctggaagc tatcccactc gaagatctgc tgaataatcc
     3421 attcaattac gaggtggacc acatcatccc tagatccgta agctttgaca attccttcaa
     3481 taacaaagtt ctggttaaac aggaggaaaa ttctaaaaaa gggaaccgga ccccgttcca
     3541 gtacctgagc tccagtgaca gcaagattag ctacgagact tttaagaaac atattctgaa
     3601 tctggccaaa ggcaaaggca ggatcagcaa gaccaagaag gagtacctcc tcgaagaacg
     3661 cgacattaac agatttagtg tgcagaaaga tttcatcaac cgaaaccttg tcgatactcg
     3721 gtacgccacg agaggcctga tgaatctcct caggagctac ttccgcgtca ataatctgga
     3781 cgttaaagtc aagagcataa atgggggatt caccagcttt ctgaggagaa agtggaagtt
     3841 taagaaggaa cgaaacaaag gatacaagca ccatgctgag gatgctttga tcatcgctaa
     3901 cgcggacttt atctttaagg aatggaaaaa gctggataag gcaaagaaag tgatggaaaa
     3961 ccagatgttc gaggagaagc aggcagagtc aatgcctgag atcgagacag agcaggaata
     4021 caaggaaatt ttcatcaccc ctcatcagat taaacacata aaggacttca aagactataa
     4081 atactctcat agggtggaca aaaaacccaa tcgcaagctc attaatgaca ccctgtactc
     4141 aacacggaag gatgataaag gtaatacctt gattgtgaat aatcttaatg gattgtatga
     4201 caaagataac gacaagctca agaagctgat caacaagtct ccagagaagc tccttatgta
     4261 tcaccacgac ccacagactt atcagaaatt gaaactgatc atggagcaat acggggatga
     4321 gaagaaccca ctctacaaat attatgagga aacaggtaat tacctgacca agtactccaa
     4381 gaaggataac ggaccagtga tcaaaaagat aaagtactat ggcaacaaac ttaatgcgca
     4441 tttggacata actgacgatt accccaattc tcgaaacaag gttgtgaagc tctccctgaa
     4501 gccttataga tttgacgtgt acctggataa tggggtttat aaattcgtca ccgtgaaaaa
     4561 tctggacgtg atcaaaaagg agaactatta tgaagtaaac tcaaagtgct atgaggaggc
     4621 gaagaagctg aagaagatct ccaatcaggc cgagttcatc gcttccttct ataagaacga
     4681 tctcatcaag atcaatggag agctttatcg cgtcattggt gtgaacaatg acttgctgaa
     4741 caggatcgaa gtcaatatga tagacattac ctaccgggag tatctcgaaa acatgaatga
     4801 taaacggccg cctcacatca tcaagacaat cgcatctaaa actcagtcaa taaaaaagta
     4861 ctctaccgat atcctgggga atctctatga agtgaagtca aagaagcacc cacaaatcat
     4921 taaaaaaggt ggatccccca agaagaagag gaaagtctcg agcgactaca aagaccatga
     4981 cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaagt aaagcggccg
     5041 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     5101 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     5161 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     5221 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     5281 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     5341 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     5401 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     5461 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     5521 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     5581 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5641 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5701 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5761 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5821 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5881 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5941 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt
     6001 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6061 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat
     6121 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6181 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6241 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6541 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6781 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7021 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7081 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7141 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7201 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7261 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7321 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7381 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7441 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7501 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7561 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7621 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7681 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7741 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7801 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7861 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7921 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7981 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8041 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
