LOCUS       BPK2660                 2288 bp    DNA     circular     21-OCT-2015
DEFINITION  pUC19.
ACCESSION   urn.local...a-5b1hjki
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     Serial Cloner Genbank Format SerialCloner_Type=DNA
            SerialCloner_Comments=[DEFINITION]: - [LOCUS]:
            pUC19-U6-+103gRNA.seq SerialCloner_Ends=0,0,,0,.
FEATURES             Location/Qualifiers
     promoter        13..330
                     /SerialCloner_Color="&h0019C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /modified_by="User"
                     /label="U6 promoter"
     misc_feature    complement(332..337)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     misc_feature    345..350
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     misc_RNA        352..442
                     /created_by="User"
                     /modified_by="User"
                     /label="Sa-sgRNA(84)"
     rep_origin      complement(480..1153)
                     /created_by="User"
                     /label="pUC origin"
     CDS             complement(1298..2158)
                     /created_by="User"
                     /label="AmpR"
     promoter        complement(2200..2228)
                     /created_by="User"
                     /label="AmpR promoter"
ORIGIN      
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc cgagacgatt aatgcgtctc ggttttagta
      361 ctctgtaatg aaaattacag aatctactaa aacaaggcaa aatgccgtgt ttatctcgtc
      421 aacttgttgg cgagattttt ttaagcttgg gccgctcgag gtacctctct acatatgaca
      481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      961 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1201 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1261 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1321 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1381 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1441 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1501 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1561 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1621 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1681 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1741 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1801 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1861 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1921 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     1981 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2041 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2101 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2161 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2221 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2281 tgccacct
//
