LOCUS       Exported               11485 bp ds-DNA     circular SYN 14-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Meagan
  TITLE     Direct Submission
  JOURNAL   Exported Nov 15, 2017 from SnapGene 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             990..1706
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    1716..1740
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1746..7337
                     /label=BRCA1
     misc_feature    6026..6044
                     /label=siR
     protein_bind    complement(7364..7384)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    7443..7667
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7951..7998
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8006..9028
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8006..9028
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9158..9279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9328..9344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9352..9368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9376..9406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9421..9442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9730..10318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10489..11349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11350..11454)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggtgagcaa gggcgaggag ctgttcaccg
     1021 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1081 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1141 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1201 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1261 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1321 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1381 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1441 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1501 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1561 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1621 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1681 ctctcggcat ggacgagctg tacaagggcg cgcccacaag tttgtacaaa aaagcaggct
     1741 ccaccatgga tttatctgct cttcgcgttg aagaagtaca aaatgtcatt aatgctatgc
     1801 agaaaatctt agagtgtccc atctgtctgg agttgatcaa ggaacctgtc tccacaaagt
     1861 gtgaccacat attttgcaaa ttttgcatgc tgaaacttct caaccagaag aaagggcctt
     1921 cacagtgtcc tttatgtaag aatgatataa ccaaaaggag cctacaagaa agtacgagat
     1981 ttagtcaact tgttgaagag ctattgaaaa tcatttgtgc ttttcagctt gacacaggtt
     2041 tggagtatgc aaacagctat aattttgcaa aaaaggaaaa taactctcct gaacatctaa
     2101 aagatgaagt ttctatcatc caaagtatgg gctacagaaa ccgtgccaaa agacttctac
     2161 agagtgaacc cgaaaatcct tccttgcagg aaaccagtct cagtgtccaa ctctctaacc
     2221 ttggaactgt gagaactctg aggacaaagc agcggataca acctcaaaag acgtctgtct
     2281 acattgaatt gggatctgat tcttctgaag ataccgttaa taaggcaact tattgcagtg
     2341 tgggagatca agaattgtta caaatcaccc ctcaaggaac cagggatgaa atcagtttgg
     2401 attctgcaaa aaaggctgct tgtgaatttt ctgagacgga tgtaacaaat actgaacatc
     2461 atcaacccag taataatgat ttgaacacca ctgagaagcg tgcagctgag aggcatccag
     2521 aaaagtatca gggtagttct gtttcaaact tgcatgtgga gccatgtggc acaaatactc
     2581 atgccagctc attacagcat gagaacagca gtttattact cactaaagac agaatgaatg
     2641 tagaaaaggc tgaattctgt aataaaagca aacagcctgg cttagcaagg agccaacata
     2701 acagatgggc tggaagtaag gaaacatgta atgataggcg gactcccagc acagaaaaaa
     2761 aggtagatct gaatgctgat cccctgtgtg agagaaaaga atggaataag cagaaactgc
     2821 catgctcaga gaatcctaga gatactgaag atgttccttg gataacacta aatagcagca
     2881 ttcagaaagt taatgagtgg ttttccagaa gtgatgaact gttaggttct gatgactcac
     2941 atgatgggga gtctgaatca aatgccaaag tagctgatgt attggacgtt ctaaatgagg
     3001 tagatgaata ttctggttct tcagagaaaa tagacttact ggccagtgat cctcatgagg
     3061 ctttaatatg taaaagtgaa agagttcact ccaaatcagt agagagtaat attgaagaca
     3121 aaatatttgg gaaaacctat cggaagaagg caagcctccc caacttaagc catgtaactg
     3181 aaaatctaat tataggagca tttgttactg agccacagat aatacaagag cgtcccctca
     3241 caaataaatt aaagcgtaaa aggagaccta catcaggcct tcatcctgag gattttatca
     3301 agaaagcaga tttggcagtt caaaagactc ctgaaatgat aaatcaggga actaaccaaa
     3361 cggagcagaa tggtcaagtg atgaatatta ctaatagtgg tcatgagaat aaaacaaaag
     3421 gtgattctat tcagaatgag aaaaatccta acccaataga atcactcgaa aaagaatctg
     3481 ctttcaaaac gaaagctgaa cctataagca gcagtataag caatatggaa ctcgaattaa
     3541 atatccacaa ttcaaaagca cctaaaaaga ataggctgag gaggaagtct tctaccaggc
     3601 atattcatgc gcttgaacta gtagtcagta gaaatctaag cccacctaat tgtactgaat
     3661 tgcaaattga tagttgttct agcagtgaag agataaagaa aaaaaagtac aaccaaatgc
     3721 cagtcaggca cagcagaaac ctacaactca tggaaggtaa agaacctgca actggagcca
     3781 agaagagtaa caagccaaat gaacagacaa gtaaaagaca tgacagtgat actttcccag
     3841 agctgaagtt aacaaatgca cctggttctt ttactaagtg ttcaaatacc agtgaactta
     3901 aagaatttgt caatcctagc cttccaagag aagaaaaaga agagaaacta gaaacagtta
     3961 aagtgtctaa taatgctgaa gaccccaaag atctcatgtt aagtggagaa agggttttgc
     4021 aaactgaaag atctgtagag agtagcagta tttcactggt acctggtact gattatggca
     4081 ctcaggaaag tatctcgtta ctggaagtta gcactctagg gaaggcaaaa acagaaccaa
     4141 ataaatgtgt gagtcagtgt gcagcatttg aaaaccccaa gggactaatt catggttgtt
     4201 ccaaagataa tagaaatgac acagaaggct ttaagtatcc attgggacat gaagttaacc
     4261 acagtcggga aacaagcata gaaatggaag aaagtgaact tgatgctcag tatttgcaga
     4321 atacattcaa ggtttcaaag cgccagtcat ttgctctgtt ttcaaatcca ggaaatgcag
     4381 aagaggaatg tgcaacattc tctgcccact ctgggtcctt aaagaaacaa agtccaaaag
     4441 tcacttttga atgtgaacaa aaggaagaaa atcaaggaaa gaatgagtct aatatcaagc
     4501 ctgtacagac agttaatatc actgcaggct ttcctgtggt tggtcagaaa gataagccag
     4561 ttgataatgc caaatgtagt atcaaaggag gctctaggtt ttgtctatca tctcagttca
     4621 gaggcaacga aactggactc attactccaa ataaacatgg acttttacaa aacccatatc
     4681 gtataccacc actttttccc atcaagtcat ttgttaaaac taaatgtaag aaaaatctgc
     4741 tagaggaaaa ctttgaggaa cattcaatgt cacctgaaag agaaatggga aatgagaaca
     4801 ttccaagtac agtgagcaca attagccgta ataacattag agaaaatgtt tttaaaggag
     4861 ccagctcaag caatattaat gaagtaggtt ccagtactaa tgaagtgggc tccagtatta
     4921 atgaaatagg ttccagtgat gaaaacattc aagcagaact aggtagaaac agagggccaa
     4981 aattgaatgc tatgcttaga ttaggggttt tgcaacctga ggtctataaa caaagtcttc
     5041 ctggaagtaa ttgtaagcat cctgaaataa aaaagcaaga atatgaagaa gtagttcaga
     5101 ctgttaatac agatttctct ccatatctga tttcagataa cttagaacag cctatgggaa
     5161 gtagtcatac atctcaggtt tgttctgaga cacctgatga cctgttagat gatggtgaaa
     5221 taaaggaaga tactagtttt gctgaaaatg acattaagga aagttctgct gtttttagca
     5281 aaagcgtcca gagaggagag cttagcagga gtcctagccc tttcacccat acacatttgg
     5341 ctcagggtta ccgaagaggg gccaagaaat tagagtcctc agaagagaac ttatctagtg
     5401 aggatgaaga gcttccctgc ttccaacact tgttatttgg taaagtaaac aatatacctt
     5461 ctcagtctac taggcatagc accgttgcta ccgagtgtct gtctaagaac acagaggaga
     5521 atttattatc attgaagaat agcttaaatg actgcagtaa ccaggtaata ttggcaaagg
     5581 catctcagga acatcacctt agtgaggaaa caaaatgttc tgctagcttg ttttcttcac
     5641 agtgcagtga attggaagac ttgactgcaa atacaaacac ccaggatcct ttcttgattg
     5701 gttcttccaa acaaatgagg catcagtctg aaagccaggg agttggtctg agtgacaagg
     5761 aattggtttc agatgatgaa gaaagaggaa cgggcttgga agaaaataat caagaagagc
     5821 aaagcatgga ttcaaactta ggtgaagcag catctgggtg tgagagtgaa acaagcgtct
     5881 ctgaagactg ctcagggcta tcctctcaga gtgacatttt aaccactcag cagagggata
     5941 ccatgcaaca taacctgata aagctccagc aggaaatggc tgaactagaa gctgtgttag
     6001 aacagcatgg gagccagcct tctaacagct accctagtat aatcagtgac tcttctgccc
     6061 ttgaggacct gcgaaatcca gaacaaagca catcagaaaa agcagtatta acttcacaga
     6121 aaagtagtga ataccctata agccagaatc cagaaggcct ttctgctgac aagtttgagg
     6181 tgtctgcaga tagttctacc agtaaaaata aagaaccagg agtggaaagg tcatcccctt
     6241 ctaaatgccc atcattagat gataggtggt acatgcacag ttgctctggg agtcttcaga
     6301 atagaaacta cccatctcaa gaggagctca ttaaggttgt tgatgtggag gagcaacagc
     6361 tggaagagtc tgggccacac gatttgacgg aaacatctta cttgccaagg caagatctag
     6421 agggaacccc ttacctggaa tctggaatca gcctcttctc tgatgaccct gaatctgatc
     6481 cttctgaaga cagagcccca gagtcagctc gtgttggcaa cataccatct tcaacctctg
     6541 cattgaaagt tccccaattg aaagttgcag aatctgccca gggtccagct gctgctcata
     6601 ctactgatac tgctgggtat aatgcaatgg aagaaagtgt gagcagggag aagccagaat
     6661 tgacagcttc aacagaaagg gtcaacaaaa gaatgtccat ggtggtgtct ggcctgaccc
     6721 cagaagaatt tatgctcgtg tacaagtttg ccagaaaaca ccacatcact ttaactaatc
     6781 taattactga agagactact catgttgtta tgaaaacaga tgctgagttt gtgtgtgaac
     6841 ggacactgaa atattttcta ggaattgcgg gaggaaaatg ggtagttagc tatttctggg
     6901 tgacccagtc tattaaagaa agaaaaatgc tgaatgagca tgattttgaa gtcagaggag
     6961 atgtggtcaa tggaagaaac caccaaggtc caaagcgagc aagagaatcc caggacagaa
     7021 agatcttcag ggggctagaa atctgttgct atgggccctt caccaacatg cccacagatc
     7081 aactggaatg gatggtacag ctgtgtggtg cttctgtggt gaaggagctt tcatcattca
     7141 cccttggcac aggtgtccac ccaattgtgg ttgtgcagcc agatgcctgg acagaggaca
     7201 atggcttcca tgcaattggg cagatgtgtg aggcacctgt ggtgacccga gagtgggtgt
     7261 tggacagtgt agcactctac cagtgccagg agctggacac ctacctgata ccccagatcc
     7321 cccacagcca ctactgaacc cgctttcttg tacaaagtgg tttcagcttt cttgtacaaa
     7381 gtggtgtgac tcgagctgac tcgagtctag agggcccgtt taaacccgct gatcagcctc
     7441 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7501 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7561 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7621 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     7681 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     7741 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     7801 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7861 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7921 acttgattag ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct
     7981 agaaagtata ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     8041 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     8101 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     8161 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     8221 gattccggaa gtgcttgaca ttggggattt cagcgagagc ctgacctatt gcatctcccg
     8281 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     8341 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     8401 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     8461 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     8521 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     8581 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     8641 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     8701 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     8761 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     8821 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     8881 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     8941 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     9001 cagcactcgt ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc
     9061 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     9121 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     9181 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9241 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     9301 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10141 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10261 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10321 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10381 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10561 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10621 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10681 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10741 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10801 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10861 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10921 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10981 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11041 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11101 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11161 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11221 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11281 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11341 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11461 tccccgaaaa gtgccacctg acgtc
//
