LOCUS            2R3e_GUS_OcsT        6018 bp ds-DNA   circular     25-NOV-2010
DEFINITION  attL3-attR2.
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(join(88..3405,5226..6011))
                     /vntifkey="21"
                     /label=attL3
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
     misc_feature    complement(3270..3393)
                     /vntifkey="21"
                     /label=attR2
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
     promoter        complement(128..144)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer          complement(125..144)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     primer          complement(149..165)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     primer          3183..3198
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     promoter        242..264
                     /vntifkey="30"
                     /label=SP6\promoter
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     CDS             1443..2303
                     /vntifkey="4"
                     /label=Amp(R)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    166..166
                     /vntifkey="21"
                     /label=insertionsite
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
     terminator      5235..6000
                     /vntifkey="43"
                     /label=OcsT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     CDS             3414..5225
                     /label=GUS
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
ORIGIN
        1 ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
       61 actatcagtc aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga
      121 tatcccctat agtgagtcgt attacatggt catagctgtt tcctgtatca ctagtgaatt
      181 cgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg
      241 agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg
      301 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      361 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      421 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      481 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1081 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1741 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2401 gttccgcgca catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg
     2461 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     2521 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     2581 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     2641 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     2701 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     2761 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     2821 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     2881 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     2941 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     3001 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     3061 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     3121 agggcgaatt gggcccgacg tcgcatgctc ccggccgcca tggcggccgc gggaattcga
     3181 ttgtaaaacg acggccagtc ttaagctcgg gccctgcagc tctagagctc gaattctaca
     3241 ggtcactaat accatctaag tagttgattc atagtgactg catatgttgt gttttacagt
     3301 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     3361 acgtttctcg ttcaactttc ttgTACaaaG TGgggATCca aGCTtcggga GGTatgttac
     3421 gtcctgtaga aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc
     3481 tggatcgcga aaactgtgga attgatcagc gttggtggga aagcgcgtta caagaaagcc
     3541 gggcaattgc tgtgccaggc agttttaacg atcagttcgc cgatgcagat attcgtaatt
     3601 atgcgggcaa cgtctggtat cagcgcgaag tctttatacc gaaaggttgg gcaggccagc
     3661 gtatcgtgct gcgtttcgat gcggtcactc attacggcaa agtgtgggtc aataatcagg
     3721 aagtgatgga gcatcagggc ggctatacgc catttgaagc cgatgtcacg ccgtatgtta
     3781 ttgccgggaa aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac tggcagacta
     3841 tcccgccggg aatggtgatt accgacgaaa acggcaagaa aaagcagtct tacttccatg
     3901 atttctttaa ctatgccgga atccatcgca gcgtaatgct ctacaccacg ccgaacacct
     3961 gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg
     4021 actggcaggt ggtggccaat ggtgatgtca gcgttgaact gcgtgatgcg gatcaacagg
     4081 tggttgcaac tggacaaggc actagcggga ctttgcaagt ggtgaatccg cacctctggc
     4141 aaccgggtga aggttatctc tatgaactgt gcgtcacagc caaaagccag acagagtgtg
     4201 atatctaccc gcttcgcgtc ggcatccggt cagtggcagt gaagggcgaa cagttcctga
     4261 ttaaccacaa accgttctac tttactggct ttggtcgtca tgaagatgcg gacttgcgtg
     4321 gcaaaggatt cgataacgtg ctgatggtgc acgaccacgc attaatggac tggattgggg
     4381 ccaactccta ccgtacctcg cattaccctt acgctgaaga gatgctcgac tgggcagatg
     4441 aacatggcat cgtggtgatt gatgaaactg ctgctgtcgg ctttaacctc tctttaggca
     4501 ttggtttcga agcgggcaac aagccgaaag aactgtacag cgaagaggca gtcaacgggg
     4561 aaactcagca agcgcactta caggcgatta aagagctgat agcgcgtgac aaaaaccacc
     4621 caagcgtggt gatgtggagt attgccaacg aaccggatac ccgtccgcaa ggtgcacggg
     4681 aatatttcgc gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct
     4741 gcgtcaatgt aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc
     4801 tgtgcctgaa ccgttattac ggatggtatg tccaaagcgg cgatttggaa acggcagaga
     4861 aggtactgga aaaagaactt ctggcctggc aggagaaact gcatcagccg attatcatca
     4921 ccgaatacgg cgtggatacg ttagccgggc tgcactcaat gtacaccgac atgtggagtg
     4981 aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg
     5041 tcgtcggtga acaggtatgg aatttcgccg attttgcgac ctcgcaaggc atattgcgcg
     5101 ttggcggtaa caagaaaggg atcttcactc gcgaccgcaa accgaagtcg gcggcttttc
     5161 tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa accgcagcag ggaggcaaac
     5221 aatgactcga ggtcctgctt taatgagata tgcgagacgc ctatgatcgc atgatatttg
     5281 ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc
     5341 gccggtttcg gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa
     5401 tattctccgt tcaatttact gattgtaccc tactacttat atgtacaata ttaaaatgaa
     5461 aacaatatat tgtgctgaat aggtttatag cgacatctat gatagagcgc cacaataaca
     5521 aacaattgcg ttttattatt acaaatccaa ttttaaaaaa agcggcagaa ccggtcaaac
     5581 ctaaaagact gattacataa atcttattca aatttcaaaa ggccccaggg gctagtatct
     5641 acgacacacc gagcggcgaa ctaataacgt tcactgaagg gaactccggt tccccgccgg
     5701 cgcgcatggg tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg
     5761 ggcaccattc aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt
     5821 atgcagcatt tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt
     5881 gacgacaaat cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca
     5941 ggacctgcag gcatgcaagc tagcttacta gtgatgcata ttctatagtg tcacctaaat
     6001 ctgcggccgc caacttta    
//
