LOCUS       Entry\Clone/pGEM        4625 bp    DNA     circular     17-JAN-2007
DEFINITION  attL3-attR2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|435605775|
COMMENT     VNTDBDATE|435605775|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Entry Clone/pGEMteasyP2RP3/4glyVenusYFP-3AT|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 3207 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 177 0 3174 0 3232 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 96 0 3159 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 195 0 3219 0 3614 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 3136 0 3214 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
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COMMENT             (CStringList "/invitrogen=\"2370000\"") 1 1 1 1 
COMMENT             "complement(2542..2558)") 
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COMMENT             "complement(2539..2558)") 
COMMENT        #17=(CFSignal (CObList) "M13 reverse primer" 27 0 1 149 165 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2010000\"") 1 1 1 1 
COMMENT             "complement(2563..2579)") 
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COMMENT             "62..77") 
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COMMENT        #20=(CFSignal (CObList) "Amp(R)" 4 0 0 1443 2303 1 (CStringList) 
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COMMENT        #22=(CFSignal (CObList) "3AT" 43 0 0 4136 4617 1 (CStringList) 
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COMMENT        #23=(CFSignal (CObList) "YFP(Venus)" 4 0 0 3407 4124 1 (CStringList) 
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COMMENT                          "attL3-attR2" 1) 
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COMMENT                          "Created: 01/17/07 05:36PM" 1) 
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COMMENT                          "Last Modified: 01/17/07 05:36PM" 1) 
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COMMENT                          "length: 4625 bp" 1) 
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COMMENT                          "If necessary, linearize attB sequence nosT-LexA-Venus-YFP-3AT at a unique REN site outside the attB region. Ethanol precipitate and resuspend." 
COMMENT                          1) 
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COMMENT                          "Mix linearized attB sequence nosT-LexA-Venus-YFP-3AT and pDONR Vector pGEMteasyP2RP3 in BP buffer and TE buffers." 
COMMENT                          1) 
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COMMENT                          "Thaw, vortex and add BP Clonase enzyme. Incubate >= 1 hr. at 25\260." 
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COMMENT                          "Add Proteinase K for 10 min at 37\260." 1) 
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COMMENT                          1) 
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COMMENT                          "Select Entry Clones on agar plates with antibiotic ampicillin." 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Pst}I (195)" "@N (@S)" 5 13.5718 
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FEATURES             Location/Qualifiers
     misc_feature    complement(4618..88)
                     /vntifkey="21"
                     /label=attL3
     misc_feature    complement(3270..3392)
                     /vntifkey="21"
                     /label=attR2
     promoter        complement(128..144)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     primer          complement(125..144)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     primer          complement(149..165)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
     primer          3183..3198
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     promoter        242..264
                     /vntifkey="30"
                     /label=SP6\promoter
     CDS             1443..2303
                     /vntifkey="4"
                     /label=Amp(R)
     misc_feature    166..166
                     /vntifkey="21"
                     /label=insertionsite
     terminator      4136..4617
                     /vntifkey="43"
                     /label=3AT
     CDS             3407..4124
                     /vntifkey="4"
                     /label=YFP(Venus)
     CDS             3393..3404
                     /vntifkey="4"
                     /label=4xgly
BASE COUNT     1184 a      1143 c      1138 g      1160 t 
ORIGIN
        1 ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga 
      121 tatcccctat agtgagtcgt attacatggt catagctgtt tcctgtatca ctagtgaatt 
      181 cgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg 
      241 agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg 
      301 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 
      361 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
      421 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 
      481 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
      541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
      601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
      661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
      721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
      781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
      841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
      901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
      961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     1021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     1081 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 
     1141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     1201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     1261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     1321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     1381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     1441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     1501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     1561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     1621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     1681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     1741 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     1801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     1861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     1921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     1981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     2041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     2101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     2161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     2221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     2281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     2341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     2401 gttccgcgca catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg 
     2461 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg 
     2521 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 
     2581 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 
     2641 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 
     2701 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 
     2761 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 
     2821 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 
     2881 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 
     2941 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 
     3001 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 
     3061 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat 
     3121 agggcgaatt gggcccgacg tcgcatgctc ccggccgcca tggcggccgc gggaattcga 
     3181 ttgtaaaacg acggccagtc ttaagctcgg gccctgcagc tctagagctc gaattctaca 
     3241 ggtcactaat accatctaag tagttgattc atagtgactg catatgttgt gttttacagt 
     3301 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt 
     3361 acgtttctcg ttcaactttc ttgtacaaag tgggtggtgg tggcgcggtg agcaagggcg 
     3421 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 
     3481 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 
     3541 agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg 
     3601 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca 
     3661 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 
     3721 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 
     3781 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 
     3841 acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact 
     3901 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga 
     3961 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt 
     4021 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 
     4081 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaactagt cgatccaggc 
     4141 ctcccagctt tcgtccgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt 
     4201 ttcattgccc acacaccaga atcctactaa gtttgagtat tatggcattg gaaaagctgt 
     4261 tttcttctat catttgttct gcttgtaatt tactgtgttc tttcagtttt tgttttcgga 
     4321 catcaaaatg caaatggatg gataagagtt aataaatgat atggtccttt tgttcattct 
     4381 caaattatta ttatctgttg tttttacttt aatgggttga atttaagtaa gaaaggaact 
     4441 aacagtgtga tattaaggtg caatgttaga catataaaac agtctttcac ctctctttgg 
     4501 ttatgtcttg aattggtttg tttcttcact tatctgtgta atcaagttta ctatgagtct 
     4561 atgatcaagt aattatgcaa tcaagttaag tacagtatag gctttttgtg tcgagggcaa 
     4621 cttta 
//
