LOCUS       2R3e_4xgly_TagRFP_        4899 bp ds-DNA   circular     29-MAR-2012
DEFINITION  attL3-attR2.
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(88..3393)
                     /vntifkey="21"
                     /label=attL3
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
     CDS             3402..3413
                     /vntifkey="4"
                     /label=4xgly
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    complement(3270..3393)
                     /vntifkey="21"
                     /label=attR2
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
     CDS             3414..4103
                     /vntifkey="4"
                     /label=tagRFP
                     /ApEinfo_fwdcolor=#910b6e
                     /ApEinfo_revcolor=#910b6e
     promoter        complement(128..144)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    3394..3399
                     /label=New Feature
                     /ApEinfo_fwdcolor=#80ffff
                     /ApEinfo_revcolor=green
     primer          complement(125..144)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    4107..4112
                     /label=New Feature(1)
                     /ApEinfo_label=New Feature
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer          complement(149..165)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    4104..4106
                     /label=Endogenous stop codon
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
     primer          3183..3198
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     promoter        242..264
                     /vntifkey="30"
                     /label=SP6\promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     CDS             1443..2303
                     /vntifkey="4"
                     /label=Amp(R)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    166..166
                     /vntifkey="21"
                     /label=insertionsite
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
     terminator      4116..4881
                     /vntifkey="43"
                     /label=OcsT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    complement(4113..4892)
                     /vntifkey="21"
                     /label=attL3(1)
                     /ApEinfo_label=attL3
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
ORIGIN
        1 ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
       61 actatcagtc aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga
      121 tatcccctat agtgagtcgt attacatggt catagctgtt tcctgtatca ctagtgaatt
      181 cgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg
      241 agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg
      301 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      361 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      421 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      481 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1081 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1741 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2401 gttccgcgca catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg
     2461 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     2521 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     2581 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     2641 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     2701 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     2761 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     2821 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     2881 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     2941 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     3001 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     3061 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     3121 agggcgaatt gggcccgacg tcgcatgctc ccggccgcca tggcggccgc gggaattcga
     3181 ttgtaaaacg acggccagtc ttaagctcgg gccctgcagc tctagagctc gaattctaca
     3241 ggtcactaat accatctaag tagttgattc atagtgactg catatgttgt gttttacagt
     3301 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     3361 acgtttctcg ttcaactttc ttgtacaaag tggggatccg cggtggtggt ggcAGCgagc
     3421 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     3481 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     3541 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     3601 acggcagcag aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     3661 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     3721 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     3781 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccaaca
     3841 ccgagatgct gtaccccgct gacggcggcc tggaaggcag aagcgacatg gccctgaagc
     3901 tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc aagaaacccg
     3961 ctaagaacct caagatgccc ggcgtctact atgtggacca cagactggaa agaatcaagg
     4021 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     4081 tccctagcaa actggggcac AAGTGActcg aggtcctgct ttaatgagat atgcgagacg
     4141 cctatgatcg catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca
     4201 tgtgtagctc agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta
     4261 ctatcgtatt tttatgaata atattctccg ttcaatttac tgattgtacc ctactactta
     4321 tatgtacaat attaaaatga aaacaatata ttgtgctgaa taggtttata gcgacatcta
     4381 tgatagagcg ccacaataac aaacaattgc gttttattat tacaaatcca attttaaaaa
     4441 aagcggcaga accggtcaaa cctaaaagac tgattacata aatcttattc aaatttcaaa
     4501 aggccccagg ggctagtatc tacgacacac cgagcggcga actaataacg ttcactgaag
     4561 ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga gtattggccg
     4621 tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg gccgggtaac
     4681 cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc ccaaatgaag
     4741 tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc gaattttgcg
     4801 acaacatgtc gaggctcagc aggaccTGCA Ggcatgcaag ctagcttact agtgatgcat
     4861 attctatagt gtcacctaaa tctgcggccg ccaacttta  
//
