LOCUS          2R3e_3xYFP_OcsT        6384 bp ds-DNA   circular     19-NOV-2010
DEFINITION  attL3-attR2.
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(join(88..3405,5592..6377))
                     /vntifkey="21"
                     /label=attL3
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
     misc_feature    3423..4176
                     /label=confirmed by sequencing
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=green
     misc_feature    complement(3270..3393)
                     /vntifkey="21"
                     /label=attR2
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
     misc_feature    complement(4866..5582)
                     /vntifkey="21"
                     /label=attL3(1)
                     /ApEinfo_label=attL3
                     /ApEinfo_fwdcolor=maroon4
                     /ApEinfo_revcolor=maroon4
     promoter        complement(128..144)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     CDS             4866..5582
                     /vntifkey="4"
                     /label=YFP(Venus)
                     /ApEinfo_fwdcolor=DarkOrange1
                     /ApEinfo_revcolor=DarkOrange1
     primer          complement(125..144)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     primer          complement(149..165)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     primer          3183..3198
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     promoter        242..264
                     /vntifkey="30"
                     /label=SP6\promoter
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    complement(4143..4839)
                     /vntifkey="21"
                     /label=attL3(2)
                     /ApEinfo_label=attL3
                     /ApEinfo_fwdcolor=maroon4
                     /ApEinfo_revcolor=maroon4
     CDS             1443..2303
                     /vntifkey="4"
                     /label=Amp(R)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    complement(4840..4859)
                     /vntifkey="21"
                     /label=attL3(3)
                     /ApEinfo_label=attL3
                     /ApEinfo_fwdcolor=maroon4
                     /ApEinfo_revcolor=maroon4
     misc_feature    166..166
                     /vntifkey="21"
                     /label=insertionsite
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
     CDS             4143..4839
                     /vntifkey="4"
                     /label=YFP(Venus)(1)
                     /ApEinfo_label=YFP(Venus)
                     /ApEinfo_fwdcolor=DarkOrange1
                     /ApEinfo_revcolor=DarkOrange1
     terminator      5601..6366
                     /vntifkey="43"
                     /label=OcsT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     CDS             4840..4859
                     /vntifkey="4"
                     /label=YFP(Venus)(2)
                     /ApEinfo_label=YFP(Venus)
                     /ApEinfo_fwdcolor=DarkOrange1
                     /ApEinfo_revcolor=DarkOrange1
     misc_feature    4143..4159
                     /label=VenusLP
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
     misc_feature    4840..4859
                     /label=VenusRP
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
     misc_feature    complement(3423..4116)
                     /vntifkey="21"
                     /label=attL3(4)
                     /ApEinfo_label=attL3
                     /ApEinfo_fwdcolor=maroon4
                     /ApEinfo_revcolor=maroon4
     misc_feature    complement(4117..4136)
                     /vntifkey="21"
                     /label=attL3(5)
                     /ApEinfo_label=attL3
                     /ApEinfo_fwdcolor=maroon4
                     /ApEinfo_revcolor=maroon4
     CDS             3423..4116
                     /vntifkey="4"
                     /label=YFP(Venus)(3)
                     /ApEinfo_label=YFP(Venus)
                     /ApEinfo_fwdcolor=DarkOrange1
                     /ApEinfo_revcolor=DarkOrange1
     CDS             4117..4136
                     /vntifkey="4"
                     /label=YFP(Venus)(4)
                     /ApEinfo_label=YFP(Venus)
                     /ApEinfo_fwdcolor=DarkOrange1
                     /ApEinfo_revcolor=DarkOrange1
     misc_feature    3423..3436
                     /label=VenusLP(1)
                     /ApEinfo_label=VenusLP
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
     misc_feature    4117..4136
                     /label=VenusRP(1)
                     /ApEinfo_label=VenusRP
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
     misc_feature    3420..3422
                     /label=confirmed by sequencing(1)
                     /ApEinfo_label=confirmed by sequencing
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=green
     misc_feature    complement(3420..3422)
                     /vntifkey="21"
                     /label=attL3(6)
                     /ApEinfo_label=attL3
                     /ApEinfo_fwdcolor=maroon4
                     /ApEinfo_revcolor=maroon4
     CDS             3420..3422
                     /vntifkey="4"
                     /label=YFP(Venus)(5)
                     /ApEinfo_label=YFP(Venus)
                     /ApEinfo_fwdcolor=DarkOrange1
                     /ApEinfo_revcolor=DarkOrange1
     misc_feature    3420..3422
                     /label=VenusLP(2)
                     /ApEinfo_label=VenusLP
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
ORIGIN
        1 ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
       61 actatcagtc aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga
      121 tatcccctat agtgagtcgt attacatggt catagctgtt tcctgtatca ctagtgaatt
      181 cgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg
      241 agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg
      301 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      361 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      421 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      481 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1081 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1741 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2401 gttccgcgca catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg
     2461 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     2521 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     2581 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     2641 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     2701 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     2761 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     2821 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     2881 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     2941 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     3001 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     3061 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     3121 agggcgaatt gggcccgacg tcgcatgctc ccggccgcca tggcggccgc gggaattcga
     3181 ttgtaaaacg acggccagtc ttaagctcgg gccctgcagc tctagagctc gaattctaca
     3241 ggtcactaat accatctaag tagttgattc atagtgactg catatgttgt gttttacagt
     3301 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     3361 acgtttctcg ttcaactttc ttgTACaaaG TGgggATCca aGCTtcgCAG gctGGATCCa
     3421 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3481 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3541 gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3601 tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     3661 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3721 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3781 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3841 agctggagta caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg
     3901 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg
     3961 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4021 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4081 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagAGAT
     4141 CCatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4201 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4261 acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca
     4321 ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
     4381 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4441 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4501 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4561 acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga
     4621 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
     4681 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4741 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4801 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagA
     4861 GATCCatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     4921 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     4981 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc
     5041 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
     5101 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     5161 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     5221 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     5281 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga
     5341 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
     5401 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     5461 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     5521 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     5581 agcatgatta actcgaggtc ctgctttaat gagatatgcg agacgcctat gatcgcatga
     5641 tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc
     5701 cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat
     5761 gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa
     5821 aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca
     5881 ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg
     5941 tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaaggcc ccaggggcta
     6001 gtatctacga cacaccgagc ggcgaactaa taacgttcac tgaagggaac tccggttccc
     6061 cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc tctaccgaaa
     6121 gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact tgctgccccg
     6181 agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag gtcaaacctt
     6241 gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac atgtcgaggc
     6301 tcagcaggac ctgcaggcat gcaagctagc ttactagtga tgcatattct atagtgtcac
     6361 ctaaatctgc ggccgccaac ttta   
//
