LOCUS       pCAM1300-kan-R4R       10445 bp    DNA     circular      1-MAY-2007
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|441047732|
COMMENT     VNTDBDATE|445278136|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCAM1300-kan-R4R3|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|NCBI Entrez|
COMMENT     VNTOAUTHORTEL|(301)496-2475|
COMMENT     VNTOAUTHORFAX|(301)480-9241|
COMMENT     VNTOAUTHOREML|info@ncbi.nlm.nih.gov|
COMMENT     VNTOAUTHORWWW|http://www3.ncbi.nlm.nih.gov/Entrez/|
COMMENT     VNTOAUTHORAD1|National Center for Biotechnology Information|
COMMENT     VNTOAUTHORAD2|National Library of Medicine|
COMMENT     VNTOAUTHORAD3|Building 38A, Room 8N805|
COMMENT     VNTOAUTHORAD4|Bethesda, MD 20894, U.S.A.|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             complement(6932..7723)
                     /vntifkey="4"
                     /label=Kan
     promoter        complement(7736..7815)
                     /vntifkey="30"
                     /label=nos
     CDS             9086..9745
                     /vntifkey="4"
                     /label=Cm(R)
     CDS             8439..8744
                     /vntifkey="4"
                     /label=ccdB
     misc_recomb     8275..8399
                     /vntifkey="86"
                     /label=attR4
     misc_recomb     complement(9854..9978)
                     /vntifkey="86"
                     /label=attR3
     misc_feature    1..220
                     /vntifkey="21"
                     /label=LacZ\3'
     misc_feature    280..305
                     /vntifkey="21"
                     /label=RB
     CDS             complement(1346..2346)
                     /vntifkey="4"
                     /label=pVS1\sta
     CDS             complement(2939..3939)
                     /vntifkey="4"
                     /label=pVS1\rep
     misc_feature    complement(4349..4609)
                     /vntifkey="21"
                     /label=pBR322\Bom
     rep_origin      complement(4749..5029)
                     /vntifkey="33"
                     /label=pBR322\Ori
     CDS             complement(5323..6114)
                     /vntifkey="4"
                     /label=KanR
     misc_feature    6539..6564
                     /vntifkey="21"
                     /label=LB
     misc_feature    10443..3
                     /vntifkey="21"
                     /label=(stu1/sma1)
     misc_feature    6627..6632
                     /vntifkey="21"
                     /label=(Xmn1/Sph1)
BASE COUNT     2527 a      2838 c      2734 g      2345 t         1 others
ORIGIN
        1 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa 
       61 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 
      121 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 
      181 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt 
      241 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc 
      301 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg 
      361 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc 
      421 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt 
      481 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg 
      541 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca 
      601 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg 
      661 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga 
      721 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc 
      781 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac 
      841 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt 
      901 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg 
      961 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc 
     1021 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg 
     1081 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc 
     1141 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc 
     1201 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga 
     1261 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt 
     1321 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc 
     1381 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc 
     1441 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg 
     1501 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa 
     1561 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg 
     1621 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat 
     1681 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa 
     1741 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc 
     1801 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg 
     1861 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata 
     1921 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg 
     1981 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt 
     2041 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg 
     2101 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac 
     2161 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat 
     2221 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac 
     2281 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca 
     2341 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca 
     2401 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat 
     2461 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa 
     2521 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta 
     2581 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg 
     2641 gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct 
     2701 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg 
     2761 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc 
     2821 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt 
     2881 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt 
     2941 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga 
     3001 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct 
     3061 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg 
     3121 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg 
     3181 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac 
     3241 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga 
     3301 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta 
     3361 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga 
     3421 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta 
     3481 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta 
     3541 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat 
     3601 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat 
     3661 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac 
     3721 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc 
     3781 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat 
     3841 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga 
     3901 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg 
     3961 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac 
     4021 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg 
     4081 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc 
     4141 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg 
     4201 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 
     4261 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 
     4321 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc 
     4381 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 
     4441 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     4501 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     4561 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     4621 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     4681 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     4741 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     4801 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     4861 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     4921 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     4981 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     5041 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 
     5101 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     5161 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     5221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     5281 aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa 
     5341 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc 
     5401 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata 
     5461 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt 
     5521 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt 
     5581 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca 
     5641 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg 
     5701 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat 
     5761 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga 
     5821 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa 
     5881 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg 
     5941 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc 
     6001 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca 
     6061 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta 
     6121 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg 
     6181 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca 
     6241 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg 
     6301 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc 
     6361 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga 
     6421 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc 
     6481 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg 
     6541 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga 
     6601 cgtttttaat gtactgaatt aacgccgaat tccggtcgat ctagtaacat agatgacacc 
     6661 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg 
     6721 tataattgcg ggactctaat caaaaaaccc atctcataaa taacgtcatg cattacatgt 
     6781 taattattac atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg 
     6841 caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct gcttgactct 
     6901 agctagagtc cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg 
     6961 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt 
     7021 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg 
     7081 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat 
     7141 tcggcaagca ggcatcgccc tgggtcacga cgagatcctc gccgtcgggc atccgcgcct 
     7201 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct 
     7261 gatcgacaag accggcttcc atccgagtac gtcctcgctc gatgcgatgt ttcgcttggt 
     7321 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga 
     7381 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc 
     7441 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa 
     7501 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttggagttca ttcagggcac 
     7561 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg 
     7621 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc 
     7681 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcctcga tcgagttgag 
     7741 agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt 
     7801 tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa 
     7861 tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc 
     7921 ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg 
     7981 gggtcataac gtgactccct taattctcat gtatcgataa cattaacgtt tacaatttcg 
     8041 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 
     8101 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 
     8161 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta 
     8221 tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttcaactt 
     8281 tgtatagaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga 
     8341 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggt 
     8401 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg 
     8461 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata 
     8521 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata 
     8581 tgcaccaccg ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg 
     8641 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga 
     8701 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt 
     8761 ctcgtcggca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg 
     8821 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct 
     8881 taccgaaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca 
     8941 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca 
     9001 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg 
     9061 cttttagtaa gccggatcct ctagattacg ccccgccctg ccactcatcg cagtactgtt 
     9121 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa 
     9181 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg 
     9241 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg 
     9301 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt 
     9361 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt 
     9421 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt 
     9481 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag 
     9541 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct 
     9601 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag 
     9661 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg 
     9721 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat 
     9781 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctgggggtg 
     9841 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt 
     9901 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa 
     9961 ctttattata catagttgga tatcgaattc ctgcagcccg gggatccact agttctagag 
    10021 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattccgagc 
    10081 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
    10141 cacaacatac gagccggaag hcataaagtg taaagcctgg ggtgcctaat gagtgagcta 
    10201 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 
    10261 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 
    10321 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
    10381 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
    10441 gaagg 
//
