LOCUS       pHEE401E               17112 bp    DNA     circular     18-APR-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     CDS             11283..12077
                     /vntifkey="4"
                     /label=KmR
     misc_feature    1..25
                     /vntifkey="21"
                     /label=RB
     misc_feature    complement(10834..10858)
                     /vntifkey="21"
                     /label=LB
     CDS             9517..10542
                     /vntifkey="4"
                     /label=Hyg
     rep_origin      12368..12648
                     /vntifkey="33"
                     /label=pBR322\origin
     terminator      7818..8452
                     /vntifkey="43"
                     /label=rbcS-E9t
     CDS             complement(15184..15813)
                     /vntifkey="4"
                     /label=pVS1-staA
     rep_origin      complement(13422..13616)
                     /vntifkey="33"
                     /label=pVS1-ori
     misc_feature    13622..13627
                     /vntifkey="21"
                     /label=Mutated\BsaI
     CDS             3542..7813
                     /vntifkey="4"
                     /label=zCas9
     CDS             13682..14755
                     /vntifkey="4"
                     /label=pVS1-RepA
     misc_feature    2184..2983
                     /vntifkey="21"
                     /label=EC1.2en
     misc_feature    2988..3535
                     /vntifkey="21"
                     /label=EC1.1p
     promoter        254..677
                     /vntifkey="29"
                     /label=U6-26p
     snoRNA          1900..1975
                     /vntifkey="105"
                     /label=sgRNA\scaffold
     terminator      1976..2167
                     /vntifkey="43"
                     /label=U6-26t
     CDS             957..1748
                     /vntifkey="4"
                     /label=SpR
     misc_feature    680..685
                     /vntifkey="21"
                     /label=BsaI
     misc_feature    1893..1898
                     /vntifkey="21"
                     /label=BsaI
BASE COUNT     4233 a      4315 c      4459 g      4105 t 
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac 
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga 
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 
      241 cagtgccaag cttcgacttg ccttccgcac aatacatcat ttcttcttag ctttttttct 
      301 tcttcttcgt tcatacagtt tttttttgtt tatcagctta cattttcttg aaccgtagct 
      361 ttcgttttct tctttttaac tttccattcg gagtttttgt atcttgtttc atagtttgtc 
      421 ccaggattag aatgattagg catcgaacct tcaagaattt gattgaataa aacatcttca 
      481 ttcttaagat atgaagataa tcttcaaaag gcccctggga atctgaaaga agagaagcag 
      541 gcccatttat atgggaaaga acaatagtat ttcttatata ggcccattta agttgaaaac 
      601 aatcttcaaa agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta 
      661 gagtcgaagt agtgattggg agaccaaccc agtggacata agcctgttcg gttcgtaagc 
      721 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg 
      781 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt 
      841 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat 
      901 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg 
      961 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc 
     1021 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc 
     1081 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa 
     1141 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga 
     1201 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc 
     1261 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct 
     1321 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata 
     1381 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc 
     1441 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg 
     1501 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa 
     1561 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc 
     1621 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc 
     1681 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg 
     1741 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac 
     1801 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg 
     1861 cttttattat ttttaagcgt gcataataag ccggtctcgg ttttagagct agaaatagca 
     1921 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt 
     1981 tttgcaaaat tttccagatc gatttcttct tcctctgttc ttcggcgttc aatttctggg 
     2041 gttttctctt cgttttctgt aactgaaacc taaaatttga cctaaaaaaa atctcaaata 
     2101 atatgattca gtggttttgt acttttcagt tagttgagtt ttgcagttcc gatgagataa 
     2161 accaatacca tggttatact agtgaataaa agcatttgcg tttggtttat cattgcgttt 
     2221 atacaaggac agagatccac tgagctggaa tagcttaaaa ccattatcag aacaaaataa 
     2281 accatttttt gttaagaatc agagcatagt aaacaacaga aacaacctaa gagaggtaac 
     2341 ttgtccaaga agatagctaa ttatatctat tttataaaag ttatcatagt ttgtaagtca 
     2401 caaaagatgc aaataacaga gaaactagga gacttgagaa tatacattct tgtatatttg 
     2461 tattcgagat tgtgaaaatt tgaccataag tttaaattct taaaaagata tatctgatct 
     2521 aggtgatggt tatagactgt aattttacca catgtttaat gatggatagt gacacacatg 
     2581 acacatcgac aacactatag catcttattt agattacaac atgaaatttt tctgtaatac 
     2641 atgtctttgt acataattta aaagtaattc ctaagaaata tatttataca aggagtttaa 
     2701 agaaaacata gcataaagtt caatgagtag taaaaaccat atacagtata tagcataaag 
     2761 ttcaatgagt ttattacaaa agcattggtt cactttctgt aacacgacgt taaaccttcg 
     2821 tctccaatag gagcgctact gattcaacat gccaatatat actaaatacg tttctacagt 
     2881 caaatgcttt aacgtttcat gattaagtga ctatttaccg tcaatccttt cccattcctc 
     2941 ccactaatcc aactttttaa ttactcttaa atcaccacta agctagtaac gcctatcatg 
     3001 aattagctct actaaatcta gcaacctttc aaatttgcag tattgcaggt gtctctgtgt 
     3061 ctttaaaata gttgccttat gatttcttcg gtttcaagat gatcaaatag ttatagattt 
     3121 catgctcaca catgctcatt agatgtgtac atactttact tacccaaatc tattttctcg 
     3181 caaagatttt gatggtaaag ctgatttggt tctattgaac taaatcaaac gagtttcaga 
     3241 ctgagtgatt ctaatccggc ccattagccc ctaaacagac ccactaatta cgcagctttt 
     3301 aatagagtaa ttacacctag tttacccact aaaccactaa gcactaatta tctcacaatc 
     3361 taatgagctt ccctcgtaat tacttgggct ttcactctac catttatttg taacagtcaa 
     3421 gtctctactg tctctatata aactctctaa agttaacaca caattctcat cacaaacaaa 
     3481 tcaaccaaag caacttctac tctttcttct ttcgacctta tcaatctgtt gagaaatcta 
     3541 gatggattac aaggaccacg acggggatta caaggaccac gacattgatt acaaggatga 
     3601 tgatgacaag atggctccga agaagaagag gaaggttggc atccacgggg tgccagctgc 
     3661 tgacaagaag tactcgatcg gcctcgatat tgggactaac tctgttggct gggccgtgat 
     3721 caccgacgag tacaaggtgc cctcaaagaa gttcaaggtc ctgggcaaca ccgatcggca 
     3781 ttccatcaag aagaatctca ttggcgctct cctgttcgac agcggcgaga cggctgaggc 
     3841 tacgcggctc aagcgcaccg cccgcaggcg gtacacgcgc aggaagaatc gcatctgcta 
     3901 cctgcaggag attttctcca acgagatggc gaaggttgac gattctttct tccacaggct 
     3961 ggaggagtca ttcctcgtgg aggaggataa gaagcacgag cggcatccaa tcttcggcaa 
     4021 cattgtcgac gaggttgcct accacgagaa gtaccctacg atctaccatc tgcggaagaa 
     4081 gctcgtggac tccacagata aggcggacct ccgcctgatc tacctcgctc tggcccacat 
     4141 gattaagttc aggggccatt tcctgatcga gggggatctc aacccggaca atagcgatgt 
     4201 tgacaagctg ttcatccagc tcgtgcagac gtacaaccag ctcttcgagg agaaccccat 
     4261 taatgcgtca ggcgtcgacg cgaaggctat cctgtccgct aggctctcga agtctcggcg 
     4321 cctcgagaac ctgatcgccc agctgccggg cgagaagaag aacggcctgt tcgggaatct 
     4381 cattgcgctc agcctggggc tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga 
     4441 cgccaagctg cagctctcca aggacacata cgacgatgac ctggataacc tcctggccca 
     4501 gatcggcgat cagtacgcgg acctgttcct cgctgccaag aatctgtcgg acgccatcct 
     4561 cctgtctgat attctcaggg tgaacaccga gattacgaag gctccgctct cagcctccat 
     4621 gatcaagcgc tacgacgagc accatcagga tctgaccctc ctgaaggcgc tggtcaggca 
     4681 gcagctcccc gagaagtaca aggagatctt cttcgatcag tcgaagaacg gctacgctgg 
     4741 gtacattgac ggcggggcct ctcaggagga gttctacaag ttcatcaagc cgattctgga 
     4801 gaagatggac ggcacggagg agctgctggt gaagctcaat cgcgaggacc tcctgaggaa 
     4861 gcagcggaca ttcgataacg gcagcatccc acaccagatt catctcgggg agctgcacgc 
     4921 tatcctgagg aggcaggagg acttctaccc tttcctcaag gataaccgcg agaagatcga 
     4981 gaagattctg actttcagga tcccgtacta cgtcggccca ctcgctaggg gcaactcccg 
     5041 cttcgcttgg atgacccgca agtcagagga gacgatcacg ccgtggaact tcgaggaggt 
     5101 ggtcgacaag ggcgctagcg ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa 
     5161 cctgccaaat gagaaggtgc tccctaagca ctcgctcctg tacgagtact tcacagtcta 
     5221 caacgagctg actaaggtga agtatgtgac cgagggcatg aggaagccgg ctttcctgtc 
     5281 tggggagcag aagaaggcca tcgtggacct cctgttcaag accaaccgga aggtcacggt 
     5341 taagcagctc aaggaggact acttcaagaa gattgagtgc ttcgattcgg tcgagatctc 
     5401 tggcgttgag gaccgcttca acgcctccct ggggacctac cacgatctcc tgaagatcat 
     5461 taaggataag gacttcctgg acaacgagga gaatgaggat atcctcgagg acattgtgct 
     5521 gacactcact ctgttcgagg accgggagat gatcgaggag cgcctgaaga cttacgccca 
     5581 tctcttcgat gacaaggtca tgaagcagct caagaggagg aggtacaccg gctgggggag 
     5641 gctgagcagg aagctcatca acggcattcg ggacaagcag tccgggaaga cgatcctcga 
     5701 cttcctgaag agcgatggct tcgcgaaccg caatttcatg cagctgattc acgatgacag 
     5761 cctcacattc aaggaggata tccagaaggc tcaggtgagc ggccaggggg actcgctgca 
     5821 cgagcatatc gcgaacctcg ctggctcgcc agctatcaag aaggggattc tgcagaccgt 
     5881 gaaggttgtg gacgagctgg tgaaggtcat gggcaggcac aagcctgaga acatcgtcat 
     5941 tgagatggcc cgggagaatc agaccacgca gaagggccag aagaactcac gcgagaggat 
     6001 gaagaggatc gaggagggca ttaaggagct ggggtcccag atcctcaagg agcacccggt 
     6061 ggagaacacg cagctgcaga atgagaagct ctacctgtac tacctccaga atggccgcga 
     6121 tatgtatgtg gaccaggagc tggatattaa caggctcagc gattacgacg tcgatcatat 
     6181 cgttccacag tcattcctga aggatgactc cattgacaac aaggtcctca ccaggtcgga 
     6241 caagaaccgg ggcaagtctg ataatgttcc ttcagaggag gtcgttaaga agatgaagaa 
     6301 ctactggcgc cagctcctga atgccaagct gatcacgcag cggaagttcg ataacctcac 
     6361 aaaggctgag aggggcgggc tctctgagct ggacaaggcg ggcttcatca agaggcagct 
     6421 ggtcgagaca cggcagatca ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac 
     6481 taagtacgat gagaatgaca agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa 
     6541 gctcgtctcc gacttcagga aggatttcca gttctacaag gttcgggaga tcaacaatta 
     6601 ccaccatgcc catgacgcgt acctgaacgc ggtggtcggc acagctctga tcaagaagta 
     6661 cccaaagctc gagagcgagt tcgtgtacgg ggactacaag gtttacgatg tgaggaagat 
     6721 gatcgccaag tcggagcagg agattggcaa ggctaccgcc aagtacttct tctactctaa 
     6781 cattatgaat ttcttcaaga cagagatcac tctggccaat ggcgagatcc ggaagcgccc 
     6841 cctcatcgag acgaacggcg agacggggga gatcgtgtgg gacaagggca gggatttcgc 
     6901 gaccgtcagg aaggttctct ccatgccaca agtgaatatc gtcaagaaga cagaggtcca 
     6961 gactggcggg ttctctaagg agtcaattct gcctaagcgg aacagcgaca agctcatcgc 
     7021 ccgcaagaag gactgggatc cgaagaagta cggcgggttc gacagcccca ctgtggccta 
     7081 ctcggtcctg gttgtggcga aggttgagaa gggcaagtcc aagaagctca agagcgtgaa 
     7141 ggagctgctg gggatcacga ttatggagcg ctccagcttc gagaagaacc cgatcgattt 
     7201 cctggaggcg aagggctaca aggaggtgaa gaaggacctg atcattaagc tccccaagta 
     7261 ctcactcttc gagctggaga acggcaggaa gcggatgctg gcttccgctg gcgagctgca 
     7321 gaaggggaac gagctggctc tgccgtccaa gtatgtgaac ttcctctacc tggcctccca 
     7381 ctacgagaag ctcaagggca gccccgagga caacgagcag aagcagctgt tcgtcgagca 
     7441 gcacaagcat tacctcgacg agatcattga gcagatttcc gagttctcca agcgcgtgat 
     7501 cctggccgac gcgaatctgg ataaggtcct ctccgcgtac aacaagcacc gcgacaagcc 
     7561 aatcagggag caggctgaga atatcattca tctcttcacc ctgacgaacc tcggcgcccc 
     7621 tgctgctttc aagtacttcg acacaactat cgatcgcaag aggtacacaa gcactaagga 
     7681 ggtcctggac gcgaccctca tccaccagtc gattaccggc ctctacgaga cgcgcatcga 
     7741 cctgtctcag ctcgggggcg acaagcggcc agcggcgacg aagaaggcgg ggcaggcgaa 
     7801 gaagaagaag tgagctcaga gctttcgttc gtatcatcgg tttcgacaac gttcgtcaag 
     7861 ttcaatgcat cagtttcatt gcgcacacac cagaatccta ctgagtttga gtattatggc 
     7921 attgggaaaa ctgtttttct tgtaccattt gttgtgcttg taatttactg tgttttttat 
     7981 tcggttttcg ctatcgaact gtgaaatgga aatggatgga gaagagttaa tgaatgatat 
     8041 ggtccttttg ttcattctca aattaatatt atttgttttt tctcttattt gttgtgtgtt 
     8101 gaatttgaaa ttataagaga tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg 
     8161 tggcctctaa tgaccgaagt taatatgagg agtaaaacac ttgtagttgt accattatgc 
     8221 ttattcacta ggcaacaaat atattttcag acctagaaaa gctgcaaatg ttactgaata 
     8281 caagtatgtc ctcttgtgtt ttagacattt atgaactttc ctttatgtaa ttttccagaa 
     8341 tccttgtcag attctaatca ttgctttata attatagtta tactcatgga tttgtagttg 
     8401 agtatgaaaa tattttttaa tgcattttat gacttgccaa ttgattgaca acgaattcgt 
     8461 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 
     8521 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 
     8581 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 
     8641 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggctagagca gcttgccaac 
     8701 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 
     8761 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 
     8821 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 
     8881 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 
     8941 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 
     9001 tcaaagcaag tggattgatg tgataacatg gtggagcacg acactctcgt ctactccaag 
     9061 aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta 
     9121 atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca 
     9181 gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt 
     9241 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg 
     9301 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact 
     9361 gacgtaaggg atgacgcaca atcccactat ccttcgcaag accttcctct atataaggaa 
     9421 gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca aatctatctc 
     9481 tctcgagctt tcgcagatcc cggggggcaa tgagatatga aaaagcctga actcaccgcg 
     9541 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc 
     9601 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg 
     9661 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca 
     9721 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg agtttagcga gagcctgacc 
     9781 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg 
     9841 cccgctgttc tacaaccggt cgcggaggct atggatgcga tcgctgcggc cgatcttagc 
     9901 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt 
     9961 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac 
    10021 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc 
    10081 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat 
    10141 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag 
    10201 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac 
    10261 ttcgagcgga ggcatccgga gcttgcagga tcgccacgac tccgggcgta tatgctccgc 
    10321 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg 
    10381 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa 
    10441 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt 
    10501 ggaaaccgac gccccagcac tcgtccgagg gcaaagaaat agagtagatg ccgaccggat 
    10561 ctgtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg 
    10621 aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt 
    10681 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag 
    10741 tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta aaaacgtccg 
    10801 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc 
    10861 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag 
    10921 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat 
    10981 gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc 
    11041 tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaccgc 
    11101 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa 
    11161 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc 
    11221 ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat 
    11281 aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg 
    11341 taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa 
    11401 acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg 
    11461 aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg 
    11521 aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg 
    11581 aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca 
    11641 ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct 
    11701 tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg 
    11761 aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc 
    11821 ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag 
    11881 atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg 
    11941 acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc 
    12001 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac 
    12061 tggatgaatt gttttagtac ctagaatgca tgaccaaaat cccttaacgt gagttttcgt 
    12121 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 
    12181 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 
    12241 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 
    12301 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 
    12361 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 
    12421 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 
    12481 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 
    12541 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 
    12601 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 
    12661 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 
    12721 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 
    12781 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 
    12841 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 
    12901 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta 
    12961 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg 
    13021 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc 
    13081 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc 
    13141 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc 
    13201 accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg 
    13261 cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag 
    13321 cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag 
    13381 gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg 
    13441 ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt 
    13501 ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt 
    13561 acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa 
    13621 acagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac 
    13681 attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg 
    13741 ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca 
    13801 gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga 
    13861 tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga 
    13921 gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg 
    13981 ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact 
    14041 ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg 
    14101 ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg 
    14161 tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc 
    14221 ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct 
    14281 tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt 
    14341 cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg 
    14401 gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg 
    14461 ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa 
    14521 aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg 
    14581 gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg 
    14641 cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt 
    14701 catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga 
    14761 ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac 
    14821 gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg 
    14881 ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt 
    14941 tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc 
    15001 cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga 
    15061 cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg 
    15121 cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt 
    15181 aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc 
    15241 gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac 
    15301 gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac 
    15361 ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct 
    15421 tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat 
    15481 gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac 
    15541 agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc 
    15601 cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc 
    15661 ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc 
    15721 ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc 
    15781 gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt 
    15841 actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat 
    15901 caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc 
    15961 agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg 
    16021 ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa 
    16081 aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct 
    16141 cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc 
    16201 tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat 
    16261 gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc 
    16321 gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg 
    16381 ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg 
    16441 caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg 
    16501 gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta 
    16561 ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc 
    16621 gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct 
    16681 cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct 
    16741 ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg 
    16801 cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa 
    16861 gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca 
    16921 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga 
    16981 ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa 
    17041 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgttattct aataaacgct 
    17101 cttttctctt ag 
//
