LOCUS       pTKDP-cat               5702 bp    DNA     circular     27-OCT-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|510069787|
COMMENT     VNTDBDATE|748886373|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTKDP-cat|
COMMENT     VNTAUTHORNAME|Tom Kuhlman|
COMMENT     VNTAUTHOREML|tkuhlman@illinois.edu|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     promoter        complement(5607..5613)
                     /vntifkey="30"
                     /label=P3_P
     CDS             1778..1969
                     /vntifkey="4"
                     /label=ROP
     CDS             complement(4712..5572)
                     /vntifkey="4"
                     /label=bla
     misc_RNA        4395..4502
                     /vntifkey="53"
                     /label=RNA\I
     misc_RNA        complement(3955..4506)
                     /vntifkey="53"
                     /label=RNA\II
     CDS             2292..3713
                     /vntifkey="4"
                     /label=sacB
     promoter        191..705
                     /vntifkey="29"
                     /label=PGK\Promoter
     promoter        708..773
                     /vntifkey="30"
                     /label=EM7\Promoter
     misc_binding    1523..1540
                     /vntifkey="20"
                     /label=I-SceI\Site
     misc_feature    19..43
                     /vntifkey="21"
                     /label=Landing\Pad\Region\I
     misc_binding    1..18
                     /vntifkey="20"
                     /label=I-SceI\Site
     misc_feature    1498..1522
                     /vntifkey="21"
                     /label=Landing\Pad\Region\II
     misc_recomb     100..133
                     /vntifkey="86"
                     /label=FRT
     misc_feature    50..164
                     /vntifkey="21"
                     /label=MCS
     misc_signal     1464..1497
                     /vntifkey="87"
                     /label=FRT
     CDS             774..1430
                     /vntifkey="4"
                     /label=cat
BASE COUNT     1566 a      1423 c      1339 g      1374 t 
ORIGIN
        1 tagggataac agggtaatta cggccccaag gtccaaacgg tgaaggtacc gggccccccc 
       61 tcgaggtcga cggtatcgat aagcttgata tcgaattccg aagttcctat tctctagaaa 
      121 gtataggaac ttcaggtctg aagaggagtt tacgtccagc caagctagct tggctgcagg 
      181 tcgtcgaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct 
      241 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc 
      301 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct 
      361 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga 
      421 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg 
      481 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct 
      541 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg 
      601 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc 
      661 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcctgtt gacaattaat 
      721 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggaga 
      781 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg 
      841 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg 
      901 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc 
      961 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg 
     1021 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt 
     1081 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag 
     1141 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt 
     1201 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata 
     1261 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg 
     1321 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca 
     1381 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg tgaggggatc 
     1441 aattctctag agctcgctga tcagaagttc ctattctcta gaaagtatag gaacttcgat 
     1501 ggcgcctcat ccctgaagcc aatagggata acagggtaat gcaggatgct gctggctacc 
     1561 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct 
     1621 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc 
     1681 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac 
     1741 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc 
     1801 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga 
     1861 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct 
     1921 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 
     1981 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 
     2041 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag 
     2101 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 
     2161 atgtactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact 
     2221 tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg 
     2281 agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta 
     2341 ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat 
     2401 ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac 
     2461 agcaaaaaaa tgaaaaatat caagttcctg aattcgattc gtccacaatt aaaaatatct 
     2521 cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct gacggcactg 
     2581 tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct aaaaatgcgg 
     2641 atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt gacagctgga 
     2701 aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat tctatcctaa 
     2761 aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga aaaatccgtt 
     2821 tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca actgcacaag 
     2881 ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat tataaatcaa 
     2941 tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat gaaggcaact 
     3001 acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat aaaggccaca 
     3061 aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc gaagaatctt 
     3121 tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac 
     3181 ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc ggtatgattg 
     3241 agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca tctaacacag 
     3301 taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca 
     3361 ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat atttacatgc 
     3421 ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa actggccttg 
     3481 tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac ttcgctgtac 
     3541 ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga ggattctacg 
     3601 cagacaaaca atcaacgttt gcgccaagct tcctgctgaa catcaaaggc aagaaaacat 
     3661 ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa taacatatgc 
     3721 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt 
     3781 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
     3841 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 
     3901 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     3961 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     4021 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     4081 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     4141 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     4201 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     4261 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     4321 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     4381 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     4441 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     4501 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     4561 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 
     4621 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 
     4681 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 
     4741 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 
     4801 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 
     4861 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 
     4921 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 
     4981 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg 
     5041 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 
     5101 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 
     5161 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 
     5221 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 
     5281 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata 
     5341 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 
     5401 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 
     5461 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 
     5521 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 
     5581 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 
     5641 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 
     5701 ct 
//
