LOCUS Exported 10447 bp ds-DNA circular SYN 14-DEC-2015 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled 6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10447) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Dec 14, 2015 from SnapGene 2.8.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10447 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..635 /label=5'-LTR /note="5'-LTR" misc_feature 636..653 /label=PBS /note="PBS" misc_feature 685..822 /label=packaging signal /note="packaging signal" misc_feature 2027..2151 /label=cPPT /note="cPPT" misc_feature 2190..3471 /label=EF1a /note="EF1a" misc_feature 3488..5014 /label=EGFP:NES:EGFP /note="EGFP:NES:EGFP" misc_feature 5023..5598 /label=IRES /note="IRES" misc_feature 5609..7093 /label=2xRFP:NLS /note="2xRFP:NLS" misc_feature 7164..7800 /label=3'-LTR /note="3'-LTR" primer_bind complement(7923..7943) /label=M13-rev /note="M13-rev" misc_binding complement(7949..7971) /label=LacO /note="LacO" CDS complement(9088..9747) /label=AmpR /note="AmpR" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgcgccc gacgataagc tttgcaaaga tggataaagt 2221 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa 2281 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2341 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2401 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2461 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2521 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2581 ttacttccac tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg 2641 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg 2701 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc 2761 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg 2821 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc 2881 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg 2941 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc 3001 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc 3061 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag 3121 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc 3181 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga 3241 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat 3301 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta 3361 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac 3421 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaatttcga cccggatccg 3481 cggccgcatg gccagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga 3541 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc 3601 tacatacgga aagcttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg 3661 gccaacactt gtcactactt tctcttatgg tgttcaatgc ttttcccgtt atccggatca 3721 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac 3781 tatatctttc aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga 3841 tacccttgtt aatcgtatcg agttaaaagg tattgatttt aaagaagatg gaaacattct 3901 cggacacaaa ctcgagtaca actataactc acacaatgta tacatcacgg cagacaaaca 3961 aaagaatgga atcaaagcta acttcaaaat tcgccacaac attgaagatg gatccgttca 4021 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga 4081 caaccattac ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca 4141 catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta 4201 caaaagcggt tccggaccgg atcaaacaag tttgtacaaa aaagcaggct tactacagct 4261 accgccgctg gaaagactga ctctgggagg cgggatggtg agcaagggcg aggagctgtt 4321 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 4381 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 4441 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 4501 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 4561 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 4621 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 4681 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 4741 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 4801 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 4861 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 4921 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 4981 gatcactctc ggcatggacg agctgtacaa gtaggaattc cgcccctctc cctccccccc 5041 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt 5101 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt 5161 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa 5221 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac 5281 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg 5341 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt 5401 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca 5461 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt 5521 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg 5581 aaaaacacga tgataagctt gccgtacgat gccaaaaaag aaaagaaaag ttgtgagcaa 5641 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga 5701 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga 5761 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga 5821 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat 5881 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt 5941 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat 6001 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa 6061 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg 6121 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac 6181 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt 6241 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg 6301 ccgccactcc accggcggca tggacgagct gtacaagtcc ggactcagat ctcgagctca 6361 catcacaagt ttgtacaaaa aagcaggctt aatgagcgag ctgattaagg agaacatgca 6421 catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg 6481 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc 6541 tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca gaaccttcat 6601 caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg gcttcacatg 6661 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct 6721 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc catccaacgg 6781 ccctgtgatg cagaagaaaa cactcggctg ggaggccaac accgagatgc tgtaccccgc 6841 tgacggcggc ctggaaggca gaagcgacat ggccctgaag ctcgtgggcg ggggccacct 6901 gatctgcaac ttcaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc 6961 cggcgtctac tatgtggacc acagactgga aagaatcaag gaggccgaca aagagaccta 7021 cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca aactggggca 7081 caaacttaat tagggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 7141 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata 7201 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac 7261 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg 7321 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga 7381 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc 7441 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc 7501 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga 7561 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg 7621 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 7681 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 7741 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 7801 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 7861 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta 7921 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 7981 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 8041 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 8101 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 8161 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 8221 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 8281 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 8341 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 8401 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 8461 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 8521 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 8581 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 8641 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 8701 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 8761 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 8821 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa 8881 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8941 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 9001 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 9061 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 9121 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 9181 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 9241 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 9301 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 9361 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 9421 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 9481 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9541 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9601 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9661 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 9721 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9781 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9841 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9901 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9961 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 10021 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 10081 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata 10141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 10201 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc 10261 atcccaaact tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta 10321 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata 10381 tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact 10441 tagtagt //