LOCUS       pSTARR-seq_fly-e        5542 bp    DNA     linear       11-NOV-2015
FEATURES             Location/Qualifiers
     primer_bind     103..122
                     /dnas_title="RVprimer3 binding region"
                     /vntifkey="28"
                     /label=RVprimer3 binding region
     polyA_signal    1..154
                     /dnas_title="Synthetic (upstream) poly (A)signal"
                     /vntifkey="25"
                     /label=Synthetic (upstream) poly (A)signal
     misc_feature    162..193
                     /dnas_title="MCS"
                     /vntifkey="21"
                     /label=MCS
     gene            433..1152
                     /note="sgGFP"
     intron          352..422
                     /note="MHC intron 16"
     intron          355..421
                     /note="intron spliced out"
     promoter        209..308
                     /note="eEF1delta core promoter"
     gene            1285..1944
                     /note="CamR"
     gene            2286..2591
                     /note="ccdB survival"
     primer_bind     2946..2965
                     /dnas_title="RVprimer4 binding site"
                     /vntifkey="28"
                     /label=RVprimer4 binding site
     polyA_signal    2660..2881
                     /dnas_title="SV40 late poly (A) signal"
                     /vntifkey="25"
                     /label=SV40 late poly (A) signal
     rep_origin      3203..3203
                     /dnas_title="ColE1-derived plasmid replication origin"
                     /vntifkey="33"
                     /label=ColE1-derived plasmid replication origin
     rep_origin      3191..3810
                     /note="pBR322 rep_ori"
     gene            complement(3965..4825)
                     /dnas_title="Beta-lactamase gene (Ampr)"
                     /vntifkey="22"
                     /label=Beta-lactamase gene (Ampr)
     rep_origin      4957..5412
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     source          1..5542
                     /dnas_title="pSTARR-seq_fly-eEF1delta"
ORIGIN      
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
       61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      121 cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcttacgcg
      181 tgctagcccg ggcTCGAGAT CtGCGATCCA GTAGCAATAA AAAACGAATC CGATGGCGTA
      241 GATATCGATA TACCTGCATT CCCTTTCAAA CGTGTTATCG TATTGTTCAG CCAATTGTGC
      301 AACGTGTTcc tgcaGGGGCA TTCCGGTACT GTTGGTAAAG CCACCATGGA AAAGGTAGGT
      361 TCAACCACTG ATGCCTAGGC ACACCGAAAC GACTAACCCT AATTCTTATC CTTTACTTCA
      421 GGCCACTTAA Gcatggctag caaaggagaa gaactcttca ctggagttgt cccaattctt
      481 gttgaattag atggtgatgt taacggccac aagttctctg tcagtggaga gggtgaaggt
      541 gatgcaacat acggaaaact taccctgaag ttcatctgca ctactggcaa actgcctgtt
      601 ccctggccaa cactagtcac tactctgtgc tatggtgttc aatgcttttc aagatacccg
      661 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      721 aggaccatct tcttcaaaga tgacggcaac tacaagacac gtgctgaagt caagtttgaa
      781 ggtgataccc ttgttaatag aatcgagtta aaaggtattg acttcaagga agatggcaac
      841 attctgggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      901 aaacaaaaga atggaatcaa agCgaacttc aagacccgcc acaacattga agatggaagc
      961 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     1021 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     1081 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     1141 ctgtacaact gatCTAGAGC ATGCACCGGT GATATCgcgg ccgcattagg caccccaggc
     1201 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     1261 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     1321 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     1381 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     1441 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     1501 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     1561 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     1621 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     1681 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     1741 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     1801 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     1861 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     1921 gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag
     1981 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     2041 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
     2101 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     2161 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     2221 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     2281 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     2341 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     2401 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     2461 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     2521 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     2581 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcagGATAT CGTCGACGAA
     2641 TTCGGCCGGc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     2701 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     2761 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     2821 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     2881 aaaatcgata aggatccgac cgatgccctt gagagccttc aacccagtca gctccttccg
     2941 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     3001 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3061 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4921 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     4981 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5041 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5101 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5161 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5221 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5281 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5341 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5401 cgcttacaat ttgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     5461 gggcctcttc gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac
     5521 gggaggtact tggagcggcc gc
//

