LOCUS pGL3-Gateway-DSC 6638 bp DNA linear 30-OCT-2015 FEATURES Location/Qualifiers gene complement(1..861) /dnas_title="Beta-lactamase gene (Ampr)" /vntifkey="22" /label=Beta-lactamase gene (Ampr) rep_origin 993..1448 /dnas_title="f1 origin" /vntifkey="33" /label=f1 origin primer_bind 1681..1700 /dnas_title="RVprimer3 binding region" /vntifkey="28" /label=RVprimer3 binding region polyA_signal 1579..1732 /dnas_title="Synthetic (upstream) poly (A)signal" /vntifkey="25" /label=Synthetic (upstream) poly (A)signal misc_feature 1745..1870 /dnas_title="attR1" /vntifkey="21" /label=attR1 misc_feature 1745..3450 /note="gatewayLR cassette" gene 1979..2638 /note="CamR" gene 2980..3285 /note="ccdB survival" promoter 3463..3617 /note="DSCP Drosophila synthetic core promoter" misc_feature 3326..3450 /dnas_title="attR2" /vntifkey="21" /label=attR2 gene 3647..5299 /note="luc+ firefly" polyA_signal 5331..5552 /dnas_title="SV40 late poly (A) signal" /vntifkey="25" /label=SV40 late poly (A) signal primer_bind 5620..5639 /dnas_title="RVprimer4 binding site" /vntifkey="28" /label=RVprimer4 binding site rep_origin 5877..5877 /dnas_title="ColE1-derived plasmid replication origin" /vntifkey="33" /label=ColE1-derived plasmid replication origin rep_origin 5865..6484 /note="pBR322 ori" source 1..6638 /dnas_title="pGL3-Gateway-DSCP" ORIGIN 1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 961 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc 1021 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 1081 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 1141 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 1201 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 1261 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 1321 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 1381 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct 1441 tacaatttgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 1501 ctcttcgcta ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga 1561 ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt 1621 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa 1681 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctatcgatag 1741 gtacCacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata 1801 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc 1861 agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta 1921 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat 1981 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca 2041 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat 2101 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca 2161 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga 2221 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac 2281 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc 2341 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa 2401 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc 2461 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga 2521 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt 2581 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac 2641 gcgtggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg 2701 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg 2761 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg 2821 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg 2881 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa 2941 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc 3001 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg 3061 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc 3121 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat 3181 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa 3241 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt 3301 atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta 3361 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt 3421 ttctcgttca gctttcttgt acaaagtggt AGATCTGCGA TCgagctcgc ccggggatcg 3481 agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat cagttgtgaa tgaatgttcg 3541 agccgagcag acgtgccgct gccttcgtta atatcctttg aataagccaa ctttgaatca 3601 caagacgcat accaaacGGC ATTCCGGTAC TGTTGGTAAA GCCACcATGG AAGACGCCAA 3661 AAACATAAAG AAAGGCCCGG CGCCATTCTA TCCGCTGGAA GATGGAACCG CTGGAGAGCA 3721 ACTGCATAAG GCTATGAAGA GATACGCCCT GGTTCCTGGA ACAATTGCTT TTACAGATGC 3781 ACATATCGAG GTGGACATCA CTTACGCTGA GTACTTCGAA ATGTCCGTTC GGTTGGCAGA 3841 AGCTATGAAA CGATATGGGC TGAATACAAA TCACAGAATC GTCGTATGCA GTGAAAACTC 3901 TCTTCAATTC TTTATGCCGG TGTTGGGCGC GTTATTTATC GGAGTTGCAG TTGCGCCCGC 3961 GAACGACATT TATAATGAAC GTGAATTGCT CAACAGTATG GGCATTTCGC AGCCTACCGT 4021 GGTGTTCGTT TCCAAAAAGG GGTTGCAAAA AATTTTGAAC GTGCAAAAAA AGCTCCCAAT 4081 CATCCAAAAA ATTATTATCA TGGATTCTAA AACGGATTAC CAGGGATTTC AGTCGATGTA 4141 CACGTTCGTC ACATCTCATC TACCTCCCGG TTTTAATGAA TACGATTTTG TGCCAGAGTC 4201 CTTCGATAGG GACAAGACAA TTGCACTGAT CATGAACTCC TCTGGATCTA CTGGTCTGCC 4261 TAAAGGTGTC GCTCTGCCTC ATAGAACTGC CTGCGTGAGA TTCTCGCATG CCAGAGATCC 4321 TATTTTTGGC AATCAAATCA TTCCGGATAC TGCGATTTTA AGTGTTGTTC CATTCCATCA 4381 CGGTTTTGGA ATGTTTACTA CACTCGGATA TTTGATATGT GGATTTCGAG TCGTCTTAAT 4441 GTATAGATTT GAAGAAGAGC TGTTTCTGAG GAGCCTTCAG GATTACAAGA TTCAAAGTGC 4501 GCTGCTGGTG CCAACCCTAT TCTCCTTCTT CGCCAAAAGC ACTCTGATTG ACAAATACGA 4561 TTTATCTAAT TTACACGAAA TTGCTTCTGG TGGCGCTCCC CTCTCTAAGG AAGTCGGGGA 4621 AGCGGTTGCC AAGAGGTTCC ATCTGCCAGG TATCAGGCAA GGATATGGGC TCACTGAGAC 4681 TACATCAGCT ATTCTGATTA CACCCGAGGG GGATGATAAA CCGGGCGCGG TCGGTAAAGT 4741 TGTTCCATTT TTTGAAGCGA AGGTTGTGGA TCTGGATACC GGGAAAACGC TGGGCGTTAA 4801 TCAAAGAGGC GAACTGTGTG TGAGAGGTCC TATGATTATG TCCGGTTATG TAAACAATCC 4861 GGAAGCGACC AACGCCTTGA TTGACAAGGA TGGATGGCTA CATTCTGGAG ACATAGCTTA 4921 CTGGGACGAA GACGAACACT TCTTCATCGT TGACCGCCTG AAGTCTCTGA TTAAGTACAA 4981 AGGCTATCAG GTGGCTCCCG CTGAATTGGA ATCCATCTTG CTCCAACACC CCAACATCTT 5041 CGACGCAGGT GTCGCAGGTC TTCCCGACGA TGACGCCGGT GAACTTCCCG CCGCCGTTGT 5101 TGTTTTGGAG CACGGAAAGA CGATGACGGA AAAAGAGATC GTGGATTACG TCGCCAGTCA 5161 AGTAACAACC GCGAAAAAGT TGCGCGGAGG AGTTGTGTTT GTGGACGAAG TACCGAAAGG 5221 TCTTACCGGA AAACTCGACG CAAGAAAAAT CAGAGAGATC CTCATAAAGG CCAAGAAGGG 5281 CGGAAAGATC GCCGTGTAAt tctagagtcg gggcggccgg ccgcttcgag cagacatgat 5341 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat 5401 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt 5461 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt 5521 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc 5581 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg 5641 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct 5701 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 5761 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 5821 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 5881 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 5941 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6001 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6061 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6121 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6181 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 6241 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 6301 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 6361 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 6421 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 6481 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 6541 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 6601 tcaatctaaa gtatatatga gtaaacttgg tctgacag //