LOCUS       pGRB                    2706 bp    DNA     circular     20-AUG-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|670863020|
COMMENT     VNTDBDATE|670863020|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGRB|
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FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /vntifkey="21"
                     /label=J23119
     misc_feature    37..653
                     /vntifkey="21"
                     /label=Alpha\region
     misc_feature    655..688
                     /vntifkey="21"
                     /label=Cas9\binding
     misc_feature    689..720
                     /vntifkey="21"
                     /label=Terminator
     CDS             complement(179..499)
                     /vntifkey="4"
                     /label=Alpha
     CDS             complement(1658..2515)
                     /vntifkey="4"
                     /label=bla
     misc_feature    complement(721..1654)
                     /vntifkey="21"
                     /label=pUC18-LYF
     misc_feature    1796..1796
                     /vntifkey="21"
                     /label=A-T
BASE COUNT      690 a       670 c       677 g       669 t 
ORIGIN
        1 ttgacagcta gctcagtcct aggtataata ctagttgttt cggtgatgac ggtgaaaacc 
       61 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 
      121 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 
      181 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 
      241 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 
      301 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 
      361 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 
      421 ccagtgccaa gcttgcatgc ctgcaggtcg actctagagg atccccgggt accgagctcg 
      481 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
      541 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 
      601 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcagtttta 
      661 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc 
      721 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
      781 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
      841 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
      901 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
      961 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     1021 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     1081 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     1141 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     1201 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     1261 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     1321 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     1381 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     1441 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     1501 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     1561 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     1621 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     1681 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     1741 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgtgacc 
     1801 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     1861 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     1921 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     1981 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     2041 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     2101 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     2161 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     2221 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     2281 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     2341 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     2401 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     2461 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     2521 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     2581 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     2641 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 
     2701 cgaggc 
//
