LOCUS       pY011                   6217 bp    DNA     circular
ACCESSION   pY011
VERSION     pY011.1
KEYWORDS    .
FEATURES             Location/Qualifiers
     terminator      5156..5261
                     /label=T0
                     /vntifkey="43"
     misc_marker     complement(5287..6081)
                     /label=Kan/neoR
                     /vntifkey="22"
                     /note="encodes nptII (aka AphA, neoR), gives kan and neo
                     resistance, with native promoter in on AatII and SpeI from
                     Lutz vector pZE2PLtetO-1 MCS2"
     promoter        1224..1457
                     /label=pTrc promoter
                     /vntifkey="30"
     misc_feature    4206..4334
                     /label=dbl term
                     /vntifkey="21"
     misc_feature    3867..4181
                     /label=PCD
     CDS             85..1193
                     /label=lacIq
                     /vntifkey="4"
                     /note="lacI contains internal EcoRV site"
     rep_origin      complement(4468..5150)
                     /label=colE1
                     /vntifkey="33"
     protein_bind    1433..1453
                     /label=lacO
                     /vntifkey="31"
     misc_feature    3109..3843
                     /label=DHPR
     misc_feature    3850..3866
                     /label=rbs
     misc_feature    3092..3108
                     /label=rbs
     misc_feature    1490..3085
                     /label=tyrosine hydroxylase
     misc_feature    1473..1489
                     /label=rbs
ORIGIN
        1 gacgtcgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa
       61 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      121 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      181 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      241 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      301 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      361 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      421 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      481 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      541 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      601 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      661 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      721 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      781 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
      841 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
      901 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
      961 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1021 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1081 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1141 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agcgcgaatt
     1201 gatctggttt gacagcttat catcgactgc acggtgcacc aatgcttctg gcgtcaggca
     1261 gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct
     1321 caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa
     1381 atattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg
     1441 agcggataac aatttcagaa ttcaaaagat ctaagaagga gatatacata tgggctctct
     1501 gaatacggaa gacgtactgg agaatagcag cgcgtttggt gtgacgaacc cgctggaccc
     1561 ggaagagttt cgtcgccagg gtcacatgat tatcgacttt ctggcggact actatcgtga
     1621 cgtggagaag tatcctgtcc gtagccaggt tgaaccgggg tatctgcgca aacgtctgcc
     1681 agagaccgct ccgtataatc ctgaatctat tgagaccatt ctgcaagatg ttaccacgga
     1741 aatcattccg ggtctgacgc attggcagag cccaaattat tatgcgtatt ttccgagcag
     1801 cggctctgtc gcaggttttc tgggcgaaat gctgagcaca ggctttaacg tggttggctt
     1861 taactggatg tctagcccgg ccgctactga actggaatct gtggttatgg attggttcgg
     1921 caaaatgctg aatctgcctg aaagtttcct gttcagcggt tctggtggtg gtgtactgca
     1981 aggtacgagc tgtgaagcta ttctgtgcac actgacggcg gcccgtgatc gcaaactgaa
     2041 taaaattggc cgcgaacaca ttggccgcct ggtggtttac ggctctgacc agacccattg
     2101 cgcgctgcaa aaggcagcac aggttgccgg tattaacccg aagaactttc gcgcgatcaa
     2161 aacatttaag gagaatagtt ttggtctgag cgcagcaacc ctgcgtgaag tcattctgga
     2221 agacattgaa gctggcctga tccctctgtt cgtgtgccca acggtcggca caacctcttc
     2281 tacagccgtg gacccaatta gtccgatttg cgaagtggcg aaggaatacg aaatgtgggt
     2341 acatgtcgat gctgcttacg ccgggagcgc atgcatctgc cctgagtttc gtcatttcat
     2401 cgatggtgtt gaagaggccg atagtttctc tctgaatgcc cataaatggt tctttacgac
     2461 cctggattgt tgttgtctgt gggtaaagga cccatctgcc ctggtcaaag cactgagtac
     2521 aaacccggaa tacctgcgca acaaagcgac tgaaagccgc caggttgtgg attataaaga
     2581 ttggcaaatt gcactgagcc gtcgctttcg cagcctgaaa ctgtggatgg tgctgcgtag
     2641 ttatggcgta actaatctgc gcaattttct gcgcagccat gtcaaaatgg caaagacttt
     2701 tgaaggtctg atttgtatgg atgggcgctt cgaaattact gtgccgcgta ctttcgcgat
     2761 ggtatgtttc cgtctgctgc cgccgaaaac catcaaagtg tatgataatg gggtgcacca
     2821 aaatggtaac ggcgtagtcc cactgcgtga tgagaacgaa aacctggtac tggcgaacaa
     2881 actgaaccaa gtctacctgg agaccgttaa cgcgaccggc agtgtgtaca tgacccacgc
     2941 tgtggttggc ggggtttata tgatccgttt cgcggttggg agtaccctga ccgaagaacg
     3001 tcacgttatc tatgcgtgga aaatcctgca agagcacgcc gatctgatcc tggggaagtt
     3061 cagtgaggcg gacttcagta gttaaggatc taagaaggag atatacatat ggcagcagca
     3121 gcagcagcag gtgaagctcg tcgtgttctg gtttacggtg gtcgtggtgc actgggcagc
     3181 cgctgcgttc aggcattccg tgcgcgtaac tggtgggtgg cttccgtgga cgttgtcgag
     3241 aacgaagagg cgtccgcatc cattatcgtt aaaatgaccg attccttcac cgaacaggca
     3301 gatcaggtta ctgccgaagt tggcaaactg ctgggtgaag aaaaagtaga tgcgatcctg
     3361 tgtgttgctg gtggctgggc tggtggtaac gccaaaagca aatccctgtt caaaaactgt
     3421 gatctgatgt ggaaacagtc tatctggacc tctactattt ctagccatct ggcgactaag
     3481 cacctgaaag aaggtggtct gctgaccctg gccggtgcca aagcagctct ggatggtacg
     3541 ccgggtatga ttggttacgg catggctaaa ggcgctgtac atcagctgtg ccagtccctg
     3601 gcgggtaaaa actctggcat gccaccgggt gctgcggcga tcgcggtgct gccggtgacc
     3661 ctggataccc cgatgaatcg taaaagcatg ccggaagcgg atttctcttc ttggacgccg
     3721 ctggaatttc tggtagaaac gttccacgat tggattaccg gtaaaaaccg tccgtcttcc
     3781 ggtagcctga tccaggtagt gacgaccgaa ggtcgcacgg aactgacgcc agcctatttt
     3841 tgaactagta agaaggagat atacatatgg caggcaaagc gcaccgtctg tctgcggaag
     3901 aacgcgatca gctgctgccg aacctgcgtg cagtgggctg gaacgaactg gaaggtcgcg
     3961 atgctatctt caaacaattc cactttaagg atttcaaccg tgcgttcggc ttcatgactc
     4021 gtgttgcact gcaagcagaa aaactggatc atcacccgga atggttcaac gtttacaaca
     4081 aagtacatat taccctgagc acccacgaat gcgcaggtct gtctgaacgc gatatcaacc
     4141 tggcgtcctt catcgaacag gttgcggttt ccatgacctg aggatccaaa ctcgagtaag
     4201 gatctccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     4261 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     4321 tttctgcgtt tatacctagg gcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4381 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4441 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4501 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4561 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4621 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4681 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4741 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4801 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4861 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4921 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4981 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5041 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5101 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga ctagtgcttg
     5161 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     5221 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctctcg aaccccagag
     5281 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     5341 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     5401 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     5461 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     5521 ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc
     5581 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     5641 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     5701 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     5761 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     5821 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     5881 tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     5941 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     6001 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     6061 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcatga
     6121 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     6181 cccaacctta ccagagggcg ccccagctgg caattcc
//
