LOCUS       pMES140_(Wayne's_reconstruction_from_OMS6_and_OMS7_sequence).xdna    7059 bp      DNA     circular   10/16/15
DEFINITION
ACCESSION
VERSION
SOURCE
  ORGANISM
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    6143..7059
                     /label=part of pRS315 vector backbone [*]
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4219..6142
                     /label=part of pRS315 vector backbone [*]
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=x \
     misc_feature    1..3183
                     /label=other part of pRS315 vector backbone
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3184..3199
                     /label=presumably a cloning linker portion of EcoRI insert
                     /SerialCloner_Color=&h9999CC
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3200..3464
                     /label=upstream of NME1 region  portion of insert
                     /SerialCloner_Color=&hCC6633
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             3465..3804
                     /label=NME1 cds
                     /SerialCloner_Color=&h990000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3805..3967
                     /label=portion downstream of nme1 region but upstream of snR66  portion of insert
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4054..4218
                     /label=downstream of both nme1 region and snR66 cdsportion of insert [5']
                     /SerialCloner_Color=&hCB007D
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             3968..4053
                     /label=snR66 snoRNA cds portion of insert
                     /SerialCloner_Color=&h99CC33
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4271..4271
                     /label=not seen in pMES145 sequences but listed in theoretical pRS315 sequence
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=not seen in pMES145 sequences ( shows as absent in automated read) but listed in theoretical pRS315 sequence; OMS-6 generated sequence wasn't readable this far for pMES140 and so hard to know for certain status in pMES140 \
                     /SerialCloner_Desc=HERE I KEPT THE THEORETICAL SEQUE \
     misc_feature    2931..2934
                     /label=possible difference between pRS315 known sequence and sequence observed
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=possible difference between pRS315 known sequence and sequence I observed in the sequence obtained for pMES140 from OMS-7 primer. MY SEQUENCE SHOWS IT IS ABSENT. Shaf's sequence of pMES145 all picked up this apparent abberation. In both cases it was from  \
     misc_feature    4126..4126
                     /label=possible difference from genomic and sequence in pMES140
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=possible difference from genomic and sequence in pMES140. pMES145 also seems to show this difference.  \
                     /SerialCloner_Desc=AUTOMATED READ OF pMES140 COULD NOT DISCERN SPECIFIC NUCLEOTIDE HERE BUT LOOKING AT PEAKS IT CLEARLY SHOULD BE A NON-GENOMIC 'C'. GENOME LISTS A 'T' HER \
     misc_feature    4108..4108
                     /label=possible difference from genomic and sequence in pMES140
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=possible difference from genomic and sequence in pMES140. pMES145 also seems to show this difference.  \
                     /SerialCloner_Desc=AUTOMATED READ OF pMES140 MATCHES NON-GENOMIC 'G' SEEN IN pMES145 HERE. GENOME HAS AN 'A' HERE. \
                     /SerialCloner_Desc= \
                     /SerialCloner_Desc=I KEPT THE SEQUENCE OBSERVED IN THE AUTOMATED READS OF  \
     misc_feature    3840..3840
                     /label=possible difference from genomic and sequence in pMES140
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=possible difference from genomic and sequence in pMES140. pMES145 also seems to show this difference.  \
                     /SerialCloner_Desc=AUTOMATED READ OF pMES140 MATCHES NON-GENOMIC 'T' SEEN IN pMES145 HERE. GENOME HAS AN 'C' HERE. \
                     /SerialCloner_Desc= \
                     /SerialCloner_Desc=I KEPT THE SEQUENCE OBSERVED IN THE AUTOMATED READS OF  \
     misc_feature    3246..3246
                     /label=possible difference from genomic and sequence in pMES140
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=possible difference from genomic and sequence in pMES140. pMES145 also seems to show this difference.  \
                     /SerialCloner_Desc=AUTOMATED READ OF pMES140 MATCHES NON-GENOMIC 'C' SEEN IN pMES145 HERE. GENOME HAS AN 'G' HERE. \
                     /SerialCloner_Desc= \
                     /SerialCloner_Desc=I KEPT THE SEQUENCE OBSERVED IN THE AUTOMATED READS OF  \

ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc
      241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
     1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
     1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta
     2401 gaagttctcc tcgagggtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     3001 cagctggcga aggggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     3061 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     3121 ttggagctcc accgcggtgg cggccgctct agaactagtg gatcccccgg gctgcaggaa
     3181 ttcCTCGAGC TACGTCAGGG CTCTTCAACT AAAGTTTGAG AGGGCATTTT TTGTTCGAAC
     3241 AATATCAGTG CTAACAAGGT TGCTACCACA TCAATGAACT CTACTCCTGT ATGAACCCTA
     3301 ATTTTCCTTC ATGAAAAATG AATTTTCGAT GTTTTTCCGT GGATTTTATA TTTTTTCAAA
     3361 TATATAAAGG AGTAAGAAAA TAACTTTGTT TATCCTATGT TGCGGTAAAG AACTAAATAG
     3421 TGGTCTTCAC CTGTTTTCTA ATTTCTTCTC GTTTAAAGTT CTTCAATCCA TGACCAAAGA
     3481 ATCGTCACAA ATCGAAGCTT ACAAAATGGA GTAAAATTTT TTTTACTCAG TAATATGCTT
     3541 TGGGTTGAAA GTCTCCCACC AATTCGTATG CGGAAAACGT AATGAGATTT AAAAATTTTA
     3601 AATTGTTTAA ATCAACTCAT TAAGGAGGAT GCCCTTGGGT ATTCTGCTTC TTGACCTGGT
     3661 ACCTCTATTG CAGGGTACTG GTGTTTTCTT CGGTACTGGA TTCCGTTTGT ATGGAATCTA
     3721 AACCATAGTT ATGACGATTG CTCTTTCCCG TGCTGGATCG AGTAACCCAA TGGAGCTTAC
     3781 TATTCTTGGT CCATGGATTC ACCCCTTTTT ATTTTTTTAA CAGTTTGTTG TTTACACTAT
     3841 TTTTTAGCAG TGTAAAACTT TTGCATAGTT AAATATGCCG AACTGATCTA ATAAAAAGTC
     3901 TGAATAACTC AGATGAGTTA TTTATTGGTA AACTTGTGTA AGAAGAAGAT GGAATAGTGA
     3961 ATAATTCATC AAATGATGAA ATACCAATGC AACAGAGTCA AGCTCTGAGT TTCAAAAAGA
     4021 AACATGGACG AGATTGCTTT TTTATTACTG ACCATGATTA TTCATTACTT ACCATTGTTC
     4081 CTCATTCTAT ATAATTGATT GGGCACCGCG TAAATTCTTG AGCCTCTTCG GTAGTATTCA
     4141 ATCGCAATAT GGGGTAGTTT ACGGAAGTGC TGTAAATGAT GTCTAAAATG AACAACTATG
     4201 CGTCCTTATT GTGACAATga attcgatatc aagcttatcg ataccgtcga cctcgagggg
     4261 gggcccggta cccagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc
     4321 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatagg
     4381 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac tcacattaat
     4441 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4501 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4561 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4621 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4681 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctcgg
     4741 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4801 actataaaga taccaggcgt tcccccctgg aagctccctc gtgcgctctc ctgttccgac
     4861 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4921 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4981 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5041 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5101 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5161 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5221 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5281 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5341 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5401 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5461 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5521 gatctgtcta tttcgttcat ccatagttgc ctgactgccc gtcgtgtaga taactacgat
     5581 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5641 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5701 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5761 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5821 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5881 atcccccatg ttgtgaaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5941 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6001 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6061 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     6121 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6181 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6241 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6301 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6361 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6421 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
     6481 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
     6541 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
     6601 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
     6661 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg
     6721 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
     6781 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
     6841 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
     6901 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
     6961 gaggcgcgtg taagttacag gcaagcgatc cgtcctaaga aaccattatt atcatgacat
     7021 taacctataa aaataggcgt atcacgaggc cctttcgtc
//