LOCUS       529_pTRP_Met25p_        7362 bp    DNA     circular SYN 03-DEC-2015
DEFINITION  
ACCESSION   529_pTRP_Met25p_
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  Fragment 2
               Fragment ends: PvuII
               Fragment size: 4532
            Insert molecule:  Fragment 2
               Fragment ends: PvuII
               Fragment size: 2830
FEATURES             Location/Qualifiers
     misc_feature    181..562
                     /gene="MET25p"
                     /SECDrawAs="Region"
     CDS             569..2401
                     /gene="PP7-2xGFP"
                     /SECDrawAs="Gene"
     misc_feature    569..933
                     /gene="PP7 PS"
                     /SECDrawAs="Region"
     misc_feature    965..1678
                     /gene="GFP #1"
                     /SECDrawAs="Region"
     misc_feature    1686..2401
                     /gene="GFP #2"
                     /SECDrawAs="Region"
     misc_feature    2404..2657
                     /gene="CYC1t"
                     /SECDrawAs="Region"
     misc_signal     2422..2441
                     /gene="779"
                     /SECDrawAs="Label"
     CDS             complement (4574..5248)
                     /gene="TRP1"
                     /SECDrawAs="Gene"
     CDS             5561..6421
                     /gene="AmpR"
                     /SECDrawAs="Gene"
ORIGIN      
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggcg
       61 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
      121 cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc
      181 cggatgcaag ggttcgaatc ccttagctct cattattttt tgctttttct cttgaggtca
      241 catgatcgca aaatggcaaa tggcacgtga agctgtcgat attggggaac tgtggtggtt
      301 ggcaaatgac taattaagtt agtcaaggcg ccatcctcat gaaaactgtg taacataata
      361 accgaagtgt cgaaaaggtg gcaccttgtc caattgaaca cgctcgatga aaaaaataag
      421 atatatataa ggttaagtaa agcgtctgtt agaaaggaag tttttccttt ttcttgctct
      481 cttgtctttt catctactat ttccttcgtg taatacaggg tcgtcagata catagataca
      541 attctattac ccccatccat actctagaat gtccaaaacc atcgttcttt cggtcggcga
      601 ggctactcgc actctgactg agatccagtc caccgcagac cgtcagatct tcgaagagaa
      661 ggtcgggcct ctggtgggtc ggctgcgcct cacggcttcg ctccgtcaaa acggagccaa
      721 gaccgcgtat cgcgtcaacc taaaactgga tcaggcggac gtcgttgatt ccggacttcc
      781 gaaagtgcgc tacactcagg tatggtcgca cgacgtgaca atcgttgcga atagcaccga
      841 ggcctcgcgc aaatcgttgt acgatttgac caagtccctc gtcgcgacct cgcaggtcga
      901 agatcttgtc gtcaaccttg tgccgctggg ccgtggatcc cccgggctgc aggaattcga
      961 tatcgtgtct aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga
     1021 tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta
     1081 cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac
     1141 cttagtcact actttcggtt atggtgttca atgttttgcg agatacccag atcatatgaa
     1201 acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt
     1261 tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt
     1321 agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca
     1381 caaattggaa tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa
     1441 tggtatcaaa gttaacttca aaattagaca caacattgaa gatggttctg ttcaattagc
     1501 tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca
     1561 ttacttatcc actcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt
     1621 cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaaga
     1681 tatcgtgtct aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga
     1741 tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta
     1801 cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac
     1861 cttagtcact actttcggtt atggtgttca atgttttgcg agatacccag atcatatgaa
     1921 acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt
     1981 tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt
     2041 agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca
     2101 caaattggaa tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa
     2161 tggtatcaaa gttaacttca aaattagaca caacattgaa gatggttctg ttcaattagc
     2221 tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca
     2281 ttacttatcc actcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt
     2341 cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaata
     2401 actggtcgag tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc
     2461 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt
     2521 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac
     2581 agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc
     2641 tcgaaggctt taatttgcgg ccggtaccag cttttgttcc ctttagtgag ggttaatttc
     2701 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2761 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2821 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2881 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     2941 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3001 accgcatata tcgctgggcc attctcatga agaatatctt gaatttattg tcatattact
     3061 agttggtgtg gaagtccata tatcggtgat caatatagtg gttgacatgc tggctagtca
     3121 acattgagcc ttttgatcat gcaaatatat tacggtattt tacaatcaaa tatcaaactt
     3181 aactattgac tttataactt atttaggtgg taacattctt ataaaaaaga aaaaaattac
     3241 tgcaaaacag tactagcttt taacttgtat cctaggttat ctatgctgtc tcaccataga
     3301 gaatattacc tatttcagaa tgtatgtcca tgattcgccg ggtaaataca tataatacac
     3361 aaatctggct taataaagtc tataatatat ctcataaaga agtgctaaat tggctagtgc
     3421 tatatatttt taagaaaatt tcttttgact aagtccatat cgactttgta aaagttcact
     3481 ttagcataca tatattacac gagccagaaa ttgtaacttt tgcctaaaat cacaaattgc
     3541 aaaatttaat tgcttgcaaa aggtcacatg cttataatca acttttttaa aaatttaaaa
     3601 tactttttta ttttttattt ttaaacataa atgaaataat ttatttattg tttatgatta
     3661 ccgaaacata aaacctgctc aagaaaaaga aactgttttg tccttggaaa aaaagcacta
     3721 cctaggagcg gccaaaatgc cgaggctttc atagcttaaa ctctttacag aaaataggca
     3781 ttatagatca gttcgagttt tcttattctt ccttccggtt ttatcgtcac agttttacag
     3841 taaataagta tcacctctta gagttcgatg ataagctgtc aaacatgaga attaattcca
     3901 catgttaaaa tagtgaagga gcatgttcgg cacacagtgg accgaacgtg gggtaagtgc
     3961 actagggtcc ggttaaacgg atctcgcatt gatgaggcaa cgctaattat caacatatag
     4021 attgttatct atctgcatga acacgaaatc tttacttgac gacttgaggc tgatggtgtt
     4081 tatgcaaaga aaccactgtg tttaatatgt gtcactgttt gatattactg tcagcgtaga
     4141 agataatagt aaaagcggtt aataagtgta tttgagataa gtgtgataaa gtttttacag
     4201 cgaaaagacg ataaatacaa gaaaatgatt acgaggatac ggagagaggt atgtacatgt
     4261 gtatttatat actaagctgc cggcggttgt ttgcaagacc gagaaaaggc tagcaagaat
     4321 cgggtcattg tagcgtatgc gcctgtgaac attctcttca acaagtttga ttccattgcg
     4381 gtgaaatggt aaaagtcaac cccctgcgat gtatattttc ctgtacaatc aatcaaaaag
     4441 ccaaatgatt tagcattatc tttacatctt gttattttac agattttatg tttagatctt
     4501 ttatgcttgc ttttcaaaag gcttgcaggc aagtgcacaa acaatactta aataaatact
     4561 actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt
     4621 ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa
     4681 gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctctcgg
     4741 ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc
     4801 cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga
     4861 gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact
     4921 cttggtattc ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat
     4981 tgaccagagc caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg
     5041 gagtgcctga actattttta tatgctttta caagacttga aattttcctt gcaataaccg
     5101 ggtcaattgt tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat
     5161 ctagtgcaca ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag
     5221 aactacctgt gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt
     5281 atactcacgt gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt
     5341 cgaccgaatt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5401 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5461 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5521 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5581 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5641 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5701 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5761 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5821 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5881 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5941 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6001 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6061 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6121 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6181 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6241 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6301 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6361 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6421 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6481 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6541 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6601 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6661 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6721 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6781 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6841 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6901 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6961 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7021 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7081 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7141 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7201 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7261 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7321 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
//
