LOCUS       Exported                7614 bp ds-DNA     circular SYN 30-MAR-2018
DEFINITION  JDS246.
ACCESSION   urn.local...e-5h3b51e
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 30, 2018 from SnapGene 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    286..306
                     /label=CMV_fwd_primer
     promoter        287..356
                     /label=CMV_promoter
     promoter        377..396
                     /label=T7 prom
                     /note="/Transferred_From=pCMV-T7-Cas9 (MLM3613), 
                     pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pCRII-TOPO"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    403..412
                     /label=Kozak sequence
                     /label=nonstandard type: motif
                     /note="/Transferred_From=pCMV-T7-Cas9 (MLM3613), 
                     pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=JDS246; 
                     VC-(VC780+Csy4-Cas9)_pLKO-TRC001; 
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64; 
                     pCAG-NLS-dCas9-NLS-3xFLAG recoded; pCAG-NLS-hTtAgo 
                     (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG (BPK573); 
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A; 
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N; 
                     pCAG-VP64-NLS-hTtAgo; 
                     pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64; 
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG; 
                     pCW-Cas9 (modified); pCW-EFS-Puro-P2A-NFN-dCas9-VP64; 
                     pCW-NLS-dCas9-VP64 EFS-Puro-T2A-rTta3-WPRE; 
                     pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693; 
                     pLenti-TREminCMV-NLS-3xFLAG-NLS-dCas9-VP64 
                     EFS-Puro-T2A-rTta3-WPRE; pLenti-TREminCMV-NLS-dCas9-VP64 
                     EFS-Puro-T2A-rTta3-WPRE; pMLM3705; pMLM3705 annotation 
                     mess; pWPXLd (modified)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
     CDS             409..4512
                     /label=SpCas9-VRQR-HF1
                     /note="/Transferred_From=JDS246, 
                     VC-(VC780+Csy4-Cas9)_pLKO-TRC001, pCAG-Csy4-T2A-Cas9-VP64 
                     recoded (BPK1), pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
     misc_difference 1897..1899
                     /label=N497A
                     /note="/created_by=User"
     misc_difference 2389..2391
                     /label=R661A
                     /note="/created_by=User"
     misc_difference 2491..2493
                     /label=Q695A
                     /note="/created_by=User"
     misc_difference 3184..3186
                     /label=Q926A
                     /note="/created_by=User"
     misc_difference 3811..3813
                     /label=D1135V
                     /note="/created_by=User"
     misc_difference 4060..4062
                     /label=G1218R
                     /note="/created_by=User"
     misc_difference 4411..4413
                     /label=R1335Q
                     /note="/created_by=User"
     misc_difference 4417..4419
                     /label=T1337R
                     /note="/created_by=User"
     misc_feature    4519..4539
                     /label=SV40-NLS
                     /label=nonstandard type: ORF
                     /note="/created_by=User"
                     /note="/modified_by=User"
     gene            4519..4539
                     /label=SV40NLS
                     /note="/Transferred_From=pCMV-T7-Cas9 (MLM3613), 
                     pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    4546..4611
                     /label=3xFLAG
                     /label=nonstandard type: ORF
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(4669..4686)
                     /label=BGH_rev_primer
                     /note="/translation=MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTD
                     RHSIK 
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE 
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH 
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK 
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD 
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL 
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK 
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY 
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL 
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE 
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT 
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF 
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT 
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE 
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV 
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA 
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF 
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG 
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS 
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV 
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF 
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH 
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA 
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDGSPKKKRKVS 
                     SDYKDHDGDYKDHDIDYKDDDDKAAG*"
     terminator      4672..4899
                     /label=bGH_PA_terminator
                     /note="/translation=MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTD
                     RHSIK 
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE 
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH 
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK 
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD 
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL 
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK 
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY 
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL 
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE 
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT 
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF 
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT 
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE 
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV 
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA 
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF 
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG 
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS 
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV 
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF 
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH 
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA 
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDGSPKKKRKVS 
                     SDYKDHDGDYKDHDIDYKDDDDKAAG*"
     promoter        complement(4966..4984)
                     /label=M13_reverse_primer
                     /note="/translation=MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTD
                     RHSIK 
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE 
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH 
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK 
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD 
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL 
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK 
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY 
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL 
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE 
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT 
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF 
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT 
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE 
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV 
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA 
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF 
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG 
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS 
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV 
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF 
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH 
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA 
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDGSPKKKRKVS 
                     SDYKDHDGDYKDHDIDYKDDDDKAAG*"
     misc_feature    complement(4983..5005)
                     /label=M13_pUC_rev_primer
                     /note="/translation=MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTD
                     RHSIK 
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE 
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH 
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK 
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD 
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL 
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK 
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY 
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL 
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE 
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT 
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF 
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT 
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE 
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV 
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA 
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF 
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG 
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS 
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV 
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF 
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH 
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA 
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDGSPKKKRKVS 
                     SDYKDHDGDYKDHDIDYKDDDDKAAG*"
     misc_feature    complement(4988..5015)
                     /label=lacO reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=pCAG-hCas9-nls-3XFLAG (SQT817), 
                     pCAG-hCas9-nls-3XFLAG delta NcoI (BPK742), pCMV-T7-Cas9 
                     (MLM3613), pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=lacO reg; 
                     pCAG-Cas9-RECODED-nls-3XFLAG.xdna (SQT817); 
                     pCAG-hCas9(R1333A)-NLS-3xFLAG; 
                     pCAG-hCas9(R1333A/R1335A)-NLS-3xFLAG; 
                     pCAG-hCas9(R1333Q)-NLS-3xFLAG; 
                     pCAG-hCas9(R1333Q/R1335Q)-NLS-3xFLAG; 
                     pCAG-hCas9(R1335A)-NLS-3xFLAG; 
                     pCAG-hCas9(R1335Q)-NLS-3xFLAG; pCAG-hCas9-NLS-3xFLAG 
                     (SQT817)"
                     /note="/X-Transferred_Similarity=100.00%; 89.29%"
     promoter        complement(5025..5048)
                     /label=lac_promoter
                     /note="/Transferred_From=pCMV-T7-Cas9 (MLM3613), 
                     pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=JDS246; 
                     VC-(VC780+Csy4-Cas9)_pLKO-TRC001; pAC90-pmax-DEST (Addgene 
                     48222); pCAG-DEST (BPK88); pCAG-DEST V2; 
                     pCMV-NLS-hTtAgo-NLS-3xFLAG; pCW-Cas9 (modified); pCW-Cas9 
                     Activator; pCW-Cas9 N-F-N Activator; pCW-Cas9 N-F-N 
                     Activator-GG; pCW-EFS-Puro-P2A-NFN-dCas9-VP64; 
                     pCW-NLS-dCas9-VP64 EFS-Puro-T2A-rTta3-WPRE; pINDUCER21 
                     (ORF-EG) (modified); pInducer20 (modified); 
                     pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693; pLenti CMV Puro DEST 
                     (w118-1) (Addgene 17452); pLenti X1 Puro DEST (694-6) 
                     (Addgene 17297); pLenti-CAG-Puro-DEST; 
                     pLenti-TREminCMV-NLS-3xFLAG-NLS-dCas9-VP64 
                     EFS-Puro-T2A-rTta3-WPRE; pLenti-TREminCMV-NLS-dCas9-VP64 
                     EFS-Puro-T2A-rTta3-WPRE; 
                     pLenti-U6-DEST-EF1a-Csy4-P2A-Neo-WPRE; 
                     pLenti-U6-DEST-EF1a-Csy4-P2A-hygro; 
                     pLenti-U6-DEST-EF1a-Csy4-P2A-hygro-WPRE; 
                     pLenti-U6-DEST-EFS-Csy4-P2A-hygro; 
                     pLenti-U6-DEST-EFS-Csy4-P2A-hygro-WPRE; 
                     pLenti-U6-DEST-SpeI/NheI-P2A-Puro-WPRE; 
                     pLenti-U6-DEST-strategy2cassette; pLenti-U6-Puro-DEST 
                     (BPK162); pLenti-U6-Puro-DEST + WPRE (BPK640); 
                     pLenti-U6-Puro-DEST-WPRE; pMLM3705; pMLM3705 annotation 
                     mess; pUC19-U6-DEST (BPK84); pcDNA5/FRT; 
                     pcDNA5/FRT-tdtomato-AAVS1 site 1; pcDNA5/FRT-tdtomato-AAVS1
                     site 1 in wrong way; pcDNA5/FRT-tdtomato-AAVS1 site 2"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=MAVFIHNFKRKGGIGGYSAGERIVD
                     IIATD 
                     IQTKELQKQITKIQNFRVYYRDSRDPVWTRILYHSLSVIEKSESRVYHSLSVIEKSES 
                     RVYHSLSVIEKSESRVYHSLSVIEKSESRVYHSLSVIEKSESRVYHSLSVIEKSESRV 
                     YHSLSVIEKSESRARYPGRGRRVRWEAYISRARLVNRQIAWRRHPRCFDLHRRHRDRS 
                     SLRGPELVQCGGILQISTSLYKKAERET*; 
                     MEWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFI 
                     MIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDR 
                     SIRLVNGSRRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSS 
                     ALHNLR*; 
                     MEWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFI 
                     MIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDR 
                     SIRLVNGSRRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIPVWWNS 
                     ADINKFVQKS*"
     protein_bind    complement(5068..5080)
                     /label=catabolite activator protein (CAP) binding site
                     /bound_moiety="catabolite activator protein (CAP)"
                     /note="CAP protein binding site"
                     /note="/Transferred_From=pCMV-T7-Cas9 (MLM3613), 
                     pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pNEB193; pNEB205A; pNEB206A; 
                     pUC19"
                     /note="/X-Transferred_Similarity=100.00%"
     terminator      5158..5289
                     /label=SV40_PA_terminator
                     /note="/Transferred_From=pCMV-T7-Cas9 (MLM3613), 
                     pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    5246..5265
                     /label=EBV_rev_primer
                     /note="/Transferred_From=pCMV-T7-Cas9 (MLM3613), 
                     pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    complement(5357..5976)
                     /label=pBR322 origin
                     /note="Geneious type: origin of replication"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=pCAG-hCas9-nls-3XFLAG (SQT817), 
                     pCAG-hCas9-nls-3XFLAG delta NcoI (BPK742), pCMV-T7-Cas9 
                     (MLM3613), pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBR322 origin"
                     /note="/X-Transferred_Similarity=100.00%"
     gene            complement(6131..6991)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTD
                     RHSIK 
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE 
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH 
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK 
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD 
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL 
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK 
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY 
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL 
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE 
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT 
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF 
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT 
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE 
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV 
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA 
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF 
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG 
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS 
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV 
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF 
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH 
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA 
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDGSPKKKRKVS 
                     SDYKDHDGDYKDHDIDYKDDDDKAAG*"
     promoter        complement(6992..7096)
                     /label=AmpR promoter
                     /note="/Transferred_From=pCMV-T7-Cas9 (MLM3613), 
                     pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=JDS246; 
                     pCMV-NLS-hTtAgo-NLS-3xFLAG; 
                     pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693; pLVX-TetOne-puro; 
                     pMLM3705; pMLM3705 annotation mess"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    7026..7048
                     /label=oS280 primer
                     /note="/Transferred_From=DS-SPcas (modified), pCMV-T7-Cas9 
                     (MLM3613), pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=DS-SPcas (modified); JDS246; 
                     pCMV-NLS-hTtAgo-NLS-3xFLAG; 
                     pLV-CMVEI.EGFPDS-iNpLTRSEBBA6_0693; pMLM3705; pMLM3705 
                     annotation mess; pUC19-U6-103gRNA (MLM3636) + 1 binding 
                     site; pUC19-U6-103gRNA AAVS1 site1 for tdtomato; 
                     pUC19-U6-103gRNA AAVS1 site2 for tdtomato; 
                     pUC19-U6-103gRNA-linkerBsmBIcassette; 
                     pUC19-U6-103gRNA_(MLM3636); 
                     pUC19-U6-113-VEGFA3-sgRNA-BsmBIgRNAcassette; 
                     pUC19-U6-113-sgRNA-AAVS1 site1 for tdtomato; 
                     pUC19-U6-113-sgRNA-AAVS1 site2 for tdtomato; 
                     pUC19-U6-113-sgRNA-BsmBI (SQT1605); 
                     pUC19-U6-113-sgRNA-VEGFA site1; pUC19-U6-113-sgRNA-VEGFA 
                     site2; pUC19-U6-113-sgRNA-VEGFA site3; 
                     pUC19-U6-AttB-Csy4-VEGFA1-3 (BPK314); 
                     pUC19-U6-Csy4Site2-113-gRNA-Csy4Site2-BsmBI (SQT1607); 
                     pUC19-U6-DEST (BPK84); pUC19-U6-Ricky's gBlock; 
                     pUC19-U6-Ricky'sgBlock-AAVS1 site 1; 
                     pUC19-U6-Ricky'sgBlock-AAVS1 site 2; 
                     pUC19-U6-Ricky'sgBlock-VEGFA site 3; pcDNA5/FRT; 
                     pcDNA5/FRT-tdtomato-AAVS1 site 1; pcDNA5/FRT-tdtomato-AAVS1
                     site 1 in wrong way; pcDNA5/FRT-tdtomato-AAVS1 site 2"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    7380..129
                     /label=CMV Enhancer
                     /note="/Transferred_From=pCMV-T7-Cas9 (MLM3613), 
                     pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=3. 
                     pCAG-nls-Csy4-FokI-T2A-dCas9-recoded (C-terminal fusion) - 
                     linker variants; 6.pCAG-nls-FokI-Csy4-T2A-Cas9-recoded 
                     (N-terminal fusion) -linker variants; JDS246; 
                     pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded 
                     (BPK376); pCAG-Csy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 
                     recoded (BPK376frameshift); 
                     pCAG-Csy4-P2A-dCas9-NLS-FLAG-VP64 recoded; 
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK1); 
                     pCAG-Csy4-T2A-Cas9-VP64 recoded (BPK2a); 
                     pCAG-Csy4-T2A-Cas9-recoded (SQT834); 
                     pCAG-Csy4-T2A-FokI-dCas9-recoded-nls (JAF1484); 
                     pCAG-Csy4-T2A-KRAB-dCas9-recoded-nlsFLAG (NW9); 
                     pCAG-Csy4-T2A-NLS-FLAG-NLS-VP64-dCas9-recoded (making 
                     p125); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-KRAB recoded 
                     (BPK353); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded 
                     (BPK326); pCAG-Csy4-T2A-NLS-FLAG-NLS-dCas9-VP64 recoded NO 
                     CTERM FLAG; 
                     pCAG-Csy4-T2A-VP64-NLS/FLAG/NLS-dCas9-recoded-nlsFLAG 
                     (BPK328); pCAG-Csy4-T2A-VP64-dCas9 linker variant BbsI 
                     cassette; pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG 
                     (BPK285); pCAG-Csy4-T2A-VP64-dCas9-recoded-nlsFLAG 
                     (BPK286); pCAG-Csy4-T2A-dCas9-KRAB recoded (NW8); 
                     pCAG-Csy4-T2A-dCas9-VP64 recoded (BPK3); 
                     pCAG-Csy4-T2A-dCas9-VP64 recoded NO FLAG (BPK373 or 374?); 
                     pCAG-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64; 
                     pCAG-NLS-FLAG-NLS-dCas9-VP64 recoded; 
                     pCAG-NLS-FLAG-VP64-dCas9-recoded; 
                     pCAG-NLS-VP64-dCas9-recoded; pCAG-NLS-dCas9-NLS-3xFLAG 
                     recoded; pCAG-NLS-dCas9-VP64 recoded; pCAG-NLS-dCas9-VP64 
                     recoded CopyXXXXX; 
                     pCAG-NLS-hCsy4-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64; 
                     pCAG-NLS-hTtAgo (BPK633); pCAG-NLS-hTtAgo-NLS-3xFLAG 
                     (BPK573); pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64; 
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478A/D546A; 
                     pCAG-NLS-hTtAgo-NLS-3xFLAG-VP64 D478N/D546N; 
                     pCAG-VP64-NLS-FLAG-NLS-dCas9-recoded-NLS-FLAG (BPK329); 
                     pCAG-VP64-NLS-hTtAgo; pCAG-VP64-dCas9-recod linker variant 
                     BbsI cassette; pCAG-VP64-dCas9-recoded GGGGSx5 linker; 
                     pCAG-VP64-dCas9-recoded-NLS-FLAG (BPK278); 
                     pCAG-VP64-hCsy4-NLS-P2A-NLS-hdCas9-NLS-FLAG-VP64; 
                     pCAG-dCas9-VP64; 
                     pCAG-hCsy4-NLS-P2A-NLS-FLAG-NLS-hdCas9-NLS-FLAG-VP64 
                     (BPK553); pCAG-hCsy4-NLS-VP64-P2A-NLS-hdCas9-NLS-FLAG-VP64;
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-3xFLAG-KRAB (BPK550);
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 
                     delta175-307 (BPK544); 
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded; 
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded 
                     (BPK447); 
                     pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64-GG recoded 
                     (BPK531); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-KRAB 
                     (BPK454); pCAG-hCsy4-P2A-NLS-FLAG-NLS-dCas9-NLS-VP64 
                     recoded (BPK453); pCAG-hCsy4-P2A-NLS-dCas9-NLS-FLAG-VP64 
                     recoded (BPK546); pCAG-hCsy4-P2A-NLS-dCas9-NLS-KRAB 
                     (BPK548); pCAG-hCsy4-P2A-dCas9-NLS-FLAG-VP64 recoded 
                     (BPK422); pCAG-hCsy4-P2A-dCas9-NLS-KRAB (BPK439); 
                     pCAG-hCsy4-P2A-dCas9-NLS-VP64 recoded (BPK450); 
                     pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-FLAG-VP64 recoded 
                     (BPK428); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-KRAB 
                     (BPK444); pCAG-hCsy4-T2A-NLS-FLAG-NLS-dCas9-NLS-VP64 
                     recoded (BPK437); pCAG-hCsy4-T2A-dCas9-NLS-FLAG-VP64 
                     recoded (BPK426); pCAG-hCsy4-T2A-dCas9-NLS-KRAB (BPK442); 
                     pCAG-hCsy4-T2A-dCas9-NLS-VP64 recoded (BPK435); 
                     pCAG-hCsy4-T2A-nls-hFokI-dhCas9-nls-3XFLAG (SQT1601); 
                     pCAG-hTtAgo; pCAG-hTtAgo-NLS; pCAG-hTtAgo-NLS-3xFLAG; 
                     pCAG-nls-FokI-Csy4-T2A-dCas9-recoded (N-terminal fusion 
                     CK1891); pCMV-NLS-hTtAgo-NLS-3xFLAG; pLenti-CAG-Puro-DEST; 
                     pMLM3705; pMLM3705 annotation mess"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        complement(7429..7457)
                     /label=AmpR_promoter
                     /note="/Transferred_From=pCMV-T7-Cas9 (MLM3613), 
                     pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    7446..119
                     /label=CAG_enhancer
                     /note="/Transferred_From=pCMV-T7-Cas9 (MLM3613), 
                     pCMV-T7-Cas9 delta NcoI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Similarity=100.00%"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggataaaaag
      421 tattctattg gtttagacat cggcactaat tccgttggat gggctgtcat aaccgatgaa
      481 tacaaagtac cttcaaagaa atttaaggtg ttggggaaca cagaccgtca ttcgattaaa
      541 aagaatctta tcggtgccct cctattcgat agtggcgaaa cggcagaggc gactcgcctg
      601 aaacgaaccg cccggagaag gtatacacgt cgcaagaacc gaatatgtta cttacaagaa
      661 atttttagca atgagatggc caaagttgac gattctttct ttcaccgttt ggaagagtcc
      721 ttccttgtcg aagaggacaa gaaacatgaa cggcacccca tctttggaaa catagtagat
      781 gaggtggcat atcatgaaaa gtacccaacg atttatcacc tcagaaaaaa gctagttgac
      841 tcaactgata aagcggacct gaggttaatc tacttggctc ttgcccatat gataaagttc
      901 cgtgggcact ttctcattga gggtgatcta aatccggaca actcggatgt cgacaaactg
      961 ttcatccagt tagtacaaac ctataatcag ttgtttgaag agaaccctat aaatgcaagt
     1021 ggcgtggatg cgaaggctat tcttagcgcc cgcctctcta aatcccgacg gctagaaaac
     1081 ctgatcgcac aattacccgg agagaagaaa aatgggttgt tcggtaacct tatagcgctc
     1141 tcactaggcc tgacaccaaa ttttaagtcg aacttcgact tagctgaaga tgccaaattg
     1201 cagcttagta aggacacgta cgatgacgat ctcgacaatc tactggcaca aattggagat
     1261 cagtatgcgg acttattttt ggctgccaaa aaccttagcg atgcaatcct cctatctgac
     1321 atactgagag ttaatactga gattaccaag gcgccgttat ccgcttcaat gatcaaaagg
     1381 tacgatgaac atcaccaaga cttgacactt ctcaaggccc tagtccgtca gcaactgcct
     1441 gagaaatata aggaaatatt ctttgatcag tcgaaaaacg ggtacgcagg ttatattgac
     1501 ggcggagcga gtcaagagga attctacaag tttatcaaac ccatattaga gaagatggat
     1561 gggacggaag agttgcttgt aaaactcaat cgcgaagatc tactgcgaaa gcagcggact
     1621 ttcgacaacg gtagcattcc acatcaaatc cacttaggcg aattgcatgc tatacttaga
     1681 aggcaggagg atttttatcc gttcctcaaa gacaatcgtg aaaagattga gaaaatccta
     1741 acctttcgca taccttacta tgtgggaccc ctggcccgag ggaactctcg gttcgcatgg
     1801 atgacaagaa agtccgaaga aacgattact ccctggaatt ttgaggaagt tgtcgataaa
     1861 ggtgcgtcag ctcaatcgtt catcgagagg atgaccgcct ttgacaagaa tttaccgaac
     1921 gaaaaagtat tgcctaagca cagtttactt tacgagtatt tcacagtgta caatgaactc
     1981 acgaaagtta agtatgtcac tgagggcatg cgtaaacccg cctttctaag cggagaacag
     2041 aagaaagcaa tagtagatct gttattcaag accaaccgca aagtgacagt taagcaattg
     2101 aaagaggact actttaagaa aattgaatgc ttcgattctg tcgagatctc cggggtagaa
     2161 gatcgattta atgcgtcact tggtacgtat catgacctcc taaagataat taaagataag
     2221 gacttcctgg ataacgaaga gaatgaagat atcttagaag atatagtgtt gactcttacc
     2281 ctctttgaag atcgggaaat gattgaggaa agactaaaaa catacgctca cctgttcgac
     2341 gataaggtta tgaaacagtt aaagaggcgt cgctatacgg gctggggagc cttgtcgcgg
     2401 aaacttatca acgggataag agacaagcaa agtggtaaaa ctattctcga ttttctaaag
     2461 agcgacggct tcgccaatag gaactttatg gccctgatcc atgatgactc tttaaccttc
     2521 aaagaggata tacaaaaggc acaggtttcc ggacaagggg actcattgca cgaacatatt
     2581 gcgaatcttg ctggttcgcc agccatcaaa aagggcatac tccagacagt caaagtagtg
     2641 gatgagctag ttaaggtcat gggacgtcac aaaccggaaa acattgtaat cgagatggca
     2701 cgcgaaaatc aaacgactca gaaggggcaa aaaaacagtc gagagcggat gaagagaata
     2761 gaagagggta ttaaagaact gggcagccag atcttaaagg agcatcctgt ggaaaatacc
     2821 caattgcaga acgagaaact ttacctctat tacctacaaa atggaaggga catgtatgtt
     2881 gatcaggaac tggacataaa ccgtttatct gattacgacg tcgatcacat tgtaccccaa
     2941 tcctttttga aggacgattc aatcgacaat aaagtgctta cacgctcgga taagaaccga
     3001 gggaaaagtg acaatgttcc aagcgaggaa gtcgtaaaga aaatgaagaa ctattggcgg
     3061 cagctcctaa atgcgaaact gataacgcaa agaaagttcg ataacttaac taaagctgag
     3121 aggggtggct tgtctgaact tgacaaggcc ggatttatta aacgtcagct cgtggaaacc
     3181 cgcgccatca caaagcatgt tgcgcagata ctagattccc gaatgaatac gaaatacgac
     3241 gagaacgata agctgattcg ggaagtcaaa gtaatcactt taaagtcaaa attggtgtcg
     3301 gacttcagaa aggattttca attctataaa gttagggaga taaataacta ccaccatgcg
     3361 cacgacgctt atcttaatgc cgtcgtaggg accgcactca ttaagaaata cccgaagcta
     3421 gaaagtgagt ttgtgtatgg tgattacaaa gtttatgacg tccgtaagat gatcgcgaaa
     3481 agcgaacagg agataggcaa ggctacagcc aaatacttct tttattctaa cattatgaat
     3541 ttctttaaga cggaaatcac tctggcaaac ggagagatac gcaaacgacc tttaattgaa
     3601 accaatgggg agacaggtga aatcgtatgg gataagggcc gggacttcgc gacggtgaga
     3661 aaagttttgt ccatgcccca agtcaacata gtaaagaaaa ctgaggtgca gaccggaggg
     3721 ttttcaaagg aatcgattct tccaaaaagg aatagtgata agctcatcgc tcgtaaaaag
     3781 gactgggacc cgaaaaagta cggtggcttc gtgagcccta cagttgccta ttctgtccta
     3841 gtagtggcaa aagttgagaa gggaaaatcc aagaaactga agtcagtcaa agaattattg
     3901 gggataacga ttatggagcg ctcgtctttt gaaaagaacc ccatcgactt ccttgaggcg
     3961 aaaggttaca aggaagtaaa aaaggatctc ataattaaac taccaaagta tagtctgttt
     4021 gagttagaaa atggccgaaa acggatgttg gctagcgcca gagagcttca aaaggggaac
     4081 gaactcgcac taccgtctaa atacgtgaat ttcctgtatt tagcgtccca ttacgagaag
     4141 ttgaaaggtt cacctgaaga taacgaacag aagcaacttt ttgttgagca gcacaaacat
     4201 tatctcgacg aaatcataga gcaaatttcg gaattcagta agagagtcat cctagctgat
     4261 gccaatctgg acaaagtatt aagcgcatac aacaagcaca gggataaacc catacgtgag
     4321 caggcggaaa atattatcca tttgtttact cttaccaacc tcggcgctcc agccgcattc
     4381 aagtattttg acacaacgat agatcgcaaa cagtacagat ctaccaagga ggtgctagac
     4441 gcgacactga ttcaccaatc catcacggga ttatatgaaa ctcggataga tttgtcacag
     4501 cttgggggtg acggatcccc caagaagaag aggaaagtct cgagcgacta caaagaccat
     4561 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa ggctgcagga
     4621 tgaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
     4681 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4741 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4801 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4861 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4921 gctggggctc gataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     4981 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5041 agtgtaaagc ctagggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5101 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5161 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5221 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5281 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5341 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5401 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5461 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5521 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5581 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5641 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5701 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5761 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5821 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5881 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5941 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6001 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6061 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6121 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6181 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6241 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6301 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6361 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6421 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6481 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6541 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6601 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6661 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6721 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6781 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6841 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6901 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6961 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7021 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7081 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     7141 atcgatctcc cgatccccta gggtcgactc tcagtacaat ctgctctgat gccgcatagt
     7201 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     7261 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     7321 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     7381 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     7441 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     7501 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     7561 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatc
//
