LOCUS       pCDH-EF1-DIO-copGFP        8164 bp    DNA     circular     30-NOV-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|751902528|
COMMENT     VNTDBDATE|751902528|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    4259..4849
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    5226..5357
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1810..1928
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    4921..5154
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(6700..7560)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     misc_feature    1309..1809
                     /vntifkey="21"
                     /label=env
     misc_feature    5882..6555
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     misc_feature    1961..3209
                     /vntifkey="21"
                     /label=EF-1\promoter
     transposon      complement(3234..3267)
                     /vntifkey="44"
                     /label=Lox2722
     transposon      3318..3351
                     /vntifkey="44"
                     /label=LoxP
     transposon      4129..4162
                     /vntifkey="44"
                     /label=Lox2722
     transposon      complement(4213..4247)
                     /vntifkey="44"
                     /label=LoxP
     misc_feature    complement(3363..4118)
                     /vntifkey="21"
                     /label=copGFP
BASE COUNT     1973 a      1945 c      2225 g      2021 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca 
     1921 aaattttatc gatatcgata gctttgcaaa gatggataaa gttttaaaca gagaggaatc 
     1981 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc 
     2041 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt 
     2101 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc 
     2161 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg 
     2221 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg 
     2281 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc 
     2341 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt 
     2401 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc 
     2461 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag 
     2521 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa 
     2581 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg 
     2641 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa 
     2701 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt 
     2761 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa 
     2821 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag 
     2881 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt 
     2941 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt 
     3001 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg 
     3061 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg 
     3121 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt 
     3181 ttcttccatt tcaggtgtcg tgaggaattg gatcctctag agtcgactcc ggaataactt 
     3241 cgtataggat actttatacg aagttatgca gaatggtagc tggattgtag ctgctattag 
     3301 caatatgaaa cctcttaata acttcgtata gcatacatta tacgaagtta tggcgcgcct 
     3361 tagcgagatc cggtggagcc gggtccggcg gtgccgtcca cggcagaatt ggacgactga 
     3421 gcgcgggatc tggcgaaggc aatgggggtc ttgaaggcgt gctggtactc cacgatgccc 
     3481 agctcggtgt tgctgtgcag ctcctccacg cggcggaagg cgaacatggg gcccccgttc 
     3541 tgcaggatgc tggggtggat ggcgctcttg aagtgcatgt ggctgtccac cacgaagctg 
     3601 tagtagccgc cgtcgcgcag gctgaaggtg cgggcgaagc tgcccaccag cacgttatcg 
     3661 cccatggggt gcaggtgctc cacggtggcg ttgctgcgga tgatcttgtc ggtgaagatc 
     3721 acgctgtcct cggggaagcc ggtgcccacc accttgaagt cgccgatcac gcggccggcc 
     3781 tcgtagcggt agctgaagct cacgtgcagc acgccgccgt cctcgtactt ctcgatgcgg 
     3841 gtgttggtgt agccgccgtt gttgatggcg tgcaggaagg ggttctcgta gccgctgggg 
     3901 taggtgccga agtggtagaa gccgtagccc atcacgtggc tcagcaggta ggggctgaag 
     3961 gtcagggcgc ctttggtgct cttcatcttg ttggtcatgc ggccctgctt gggggtgccc 
     4021 tctccgccgc ccaccagctc gaactccacg ccgttcaggg tgccggtgat gcggcactcg 
     4081 atctccatgg cgggcaggcc gctctcgtcg ctctccatgg tggctagcat aacttcgtat 
     4141 aaagtatcct atacgaagtt atttgcctta acccagaaat tatcactgtt attctttaga 
     4201 atggtgcaaa gaataacttc gtataatgta tgctatacga agttatgaat tcgtcgacaa 
     4261 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 
     4321 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 
     4381 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 
     4441 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 
     4501 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 
     4561 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 
     4621 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 
     4681 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 
     4741 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 
     4801 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa 
     4861 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 
     4921 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 
     4981 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
     5041 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
     5101 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 
     5161 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 
     5221 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 
     5281 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 
     5341 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 
     5401 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 
     5461 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 
     5521 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     5581 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 
     5641 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 
     5701 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     5761 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     5821 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     5881 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     5941 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     6001 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     6061 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     6121 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     6181 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     6241 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     6301 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     6361 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
     6421 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     6481 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     6541 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     6601 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     6661 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     6721 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     6781 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     6841 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     6901 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     6961 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     7021 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     7081 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     7141 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     7201 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     7261 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     7321 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     7381 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     7441 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     7501 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     7561 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     7621 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     7681 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 
     7741 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 
     7801 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 
     7861 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 
     7921 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 
     7981 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 
     8041 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 
     8101 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 
     8161 gctg 
//
