LOCUS       pCDH-CB-copGFP-T2A-iCre        8547 bp    DNA     circular     30-NOV-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|751909657|
COMMENT     VNTDBDATE|751909657|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCDH-CB-copGFP-T2A-iCre|
COMMENT     VNTAUTHORNAME|Demo User|
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     promoter        2226..2498
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=#800000
                     /vntifkey="29"
                     /label=chicken\beta\actin\promoter
     enhancer        1941..2225
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#80ffff
                     /vntifkey="9"
                     /label=CMV\promoter
     misc_feature    3503..3565
                     /vntifkey="21"
                     /label=2A\peptide
     misc_feature    3566..4621
                     /vntifkey="21"
                     /label=iCre
     misc_feature    2741..3496
                     /vntifkey="21"
                     /label=copGFP
BASE COUNT     2131 a      2148 c      2286 g      1982 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 
     1921 ttaattaagg tacctctggt cgttacataa cttacggtaa atggcccgcc tggctgaccg 
     1981 cccaacgacc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
     2041 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 
     2101 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
     2161 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctact 
     2221 cgaggccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta 
     2281 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggggggc 
     2341 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg 
     2401 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc 
     2461 ggcggcccta taaaaagcga agcgcgcggc gggcgggagc gggatcagcc accgcggtgg 
     2521 cggccctaga gtcgatcgag gaactgaaaa accagaaagt taactggtaa gtttagtctt 
     2581 tttgtctttt atttcaggtc cccgatccgg tggtggtgca aatcaaagaa ctgctcctca 
     2641 gtggatgttg cctttacttc taggcctgta cggaagtgtt acttctgctc taaaagctgc 
     2701 ggaattgtac ccgcggccga tccaccggtc gggatccacc atggagagcg acgagagcgg 
     2761 cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga 
     2821 gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag 
     2881 caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt 
     2941 ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa 
     3001 cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag 
     3061 cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg 
     3121 cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga 
     3181 gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct 
     3241 gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat 
     3301 ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct 
     3361 gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc 
     3421 cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg 
     3481 ctccaccgga tctcgcaagc ttggcagtgg agagggcaga ggaagtctgc taacatgcgg 
     3541 tgacgtcgag gagaatcctg gcccaatggt gcccaagaag aagaggaaag tctccaacct 
     3601 gctgactgtg caccaaaacc tgcctgccct ccctgtggat gccacctctg atgaagtcag 
     3661 gaagaacctg atggacatgt tcagggacag gcaggccttc tctgaacaca cctggaagat 
     3721 gctcctgtct gtgtgcagat cctgggctgc ctggtgcaag ctgaacaaca ggaaatggtt 
     3781 ccctgctgaa cctgaggatg tgagggacta cctcctgtac ctgcaagcca gaggcctggc 
     3841 tgtgaagacc atccaacagc acctgggcca gctcaacatg ctgcacagga gatctggcct 
     3901 gcctcgccct tctgactcca atgctgtgtc cctggtgatg aggagaatca gaaaggagaa 
     3961 tgtggatgct ggggagagag ccaagcaggc cctggccttt gaacgcactg actttgacca 
     4021 agtcagatcc ctgatggaga actctgacag atgccaggac atcaggaacc tggccttcct 
     4081 gggcattgcc tacaacaccc tgctgcgcat tgccgaaatt gccagaatca gagtgaagga 
     4141 catctcccgc accgatggtg ggagaatgct gatccacatt ggcaggacca agaccctggt 
     4201 gtccacagct ggtgtggaga aggccctgtc cctgggggtt accaagctgg tggagagatg 
     4261 gatctctgtg tctggtgtgg ctgatgaccc caacaactac ctgttctgcc gggtcagaaa 
     4321 gaatggtgtg gctgcccctt ctgccacctc ccaactgtcc acccgggccc tggaagggat 
     4381 ctttgaggcc acccaccgcc tgatctatgg tgccaaggat gactctgggc agagatacct 
     4441 ggcctggtct ggccactctg ccagagtggg tgctgccagg gacatggcca gggctggtgt 
     4501 gtccatccct gaaatcatgc aggctggtgg ctggaccaat gtgaacattg tgatgaacta 
     4561 catcagaaac ctggactctg agactggggc catggtgagg ctgctcgagg atggggactg 
     4621 agcggccgcg aattcgtcga caatcaacct ctggattaca aaatttgtga aagattgact 
     4681 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 
     4741 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 
     4801 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 
     4861 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 
     4921 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 
     4981 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 
     5041 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 
     5101 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 
     5161 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 
     5221 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 
     5281 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct 
     5341 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 
     5401 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 
     5461 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 
     5521 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 
     5581 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 
     5641 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     5701 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 
     5761 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 
     5821 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 
     5881 ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg 
     5941 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 
     6001 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
     6061 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 
     6121 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     6181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     6241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     6301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     6361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     6421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     6481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     6541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     6601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     6661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     6721 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     6781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     6841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     6901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     6961 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     7021 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     7081 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     7141 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     7201 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     7261 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     7321 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     7381 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     7441 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     7501 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     7561 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     7621 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     7681 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     7741 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     7801 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     7861 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     7921 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     7981 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     8041 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 
     8101 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat 
     8161 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 
     8221 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 
     8281 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 
     8341 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg 
     8401 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 
     8461 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 
     8521 tgtaaaacga cggccagtgc caagctg 
//
