LOCUS       pCDH-CB-FLPe-P2A-tdTomato        9469 bp    DNA     circular     30-NOV-2015
SOURCE      
  ORGANISM  
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            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|751911392|
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FEATURES             Location/Qualifiers
     misc_feature    5564..6154
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    6531..6662
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1798..1916
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    6226..6459
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(8005..8865)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     misc_feature    1309..1797
                     /vntifkey="21"
                     /label=env
     misc_feature    7187..7860
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     promoter        2226..2498
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=#800000
                     /vntifkey="29"
                     /label=chicken\beta\actin\promoter
     enhancer        1941..2225
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#80ffff
                     /vntifkey="9"
                     /label=CMV\promoter
     misc_feature    2741..4042
                     /vntifkey="21"
                     /label=FLPe_ORF
     tRNA            4117..5548
                     /vntifkey="59"
                     /label=tdTomato
     misc_feature    4043..4108
                     /vntifkey="21"
                     /label=P2A
BASE COUNT     2522 a      2303 c      2437 g      2207 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 
     1921 ttaattaagg tacctctggt cgttacataa cttacggtaa atggcccgcc tggctgaccg 
     1981 cccaacgacc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
     2041 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 
     2101 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
     2161 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctact 
     2221 cgaggccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta 
     2281 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggggggc 
     2341 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg 
     2401 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc 
     2461 ggcggcccta taaaaagcga agcgcgcggc gggcgggagc gggatcagcc accgcggtgg 
     2521 cggccctaga gtcgatcgag gaactgaaaa accagaaagt taactggtaa gtttagtctt 
     2581 tttgtctttt atttcaggtc cccgatccgg tggtggtgca aatcaaagaa ctgctcctca 
     2641 gtggatgttg cctttacttc taggcctgta cggaagtgtt acttctgctc taaaagctgc 
     2701 ggaattgtac ccgcggccga tccaccggtc gggatccacc atggctccca agaagaagag 
     2761 gaaggtgatg agtcaatttg atatattatg taaaacacca cctaaggtcc tggttcgtca 
     2821 gtttgtggaa aggtttgaaa gaccttcagg ggaaaaaata gcatcatgtg ctgctgaact 
     2881 aacctattta tgttggatga ttactcataa cggaacagca atcaagagag ccacattcat 
     2941 gagctataat actatcataa gcaattcgct gagtttcgat attgtcaaca aatcactcca 
     3001 gtttaaatac aagacgcaaa aagcaacaat tctggaagcc tcattaaaga aattaattcc 
     3061 tgcttgggaa tttacaatta ttccttacaa tggacaaaaa catcaatctg atatcactga 
     3121 tattgtaagt agtttgcaat tacagttcga atcatcggaa gaagcagata agggaaatag 
     3181 ccacagtaaa aaaatgctta aagcacttct aagtgagggt gaaagcatct gggagatcac 
     3241 tgagaaaata ctaaattcgt ttgagtatac ctcgagattt acaaaaacaa aaactttata 
     3301 ccaattcctc ttcctagcta ctttcatcaa ttgtggaaga ttcagcgata ttaagaacgt 
     3361 tgatccgaaa tcatttaaat tagtccaaaa taagtatctg ggagtaataa tccagtgttt 
     3421 agtgacagag acaaagacaa gcgttagtag gcacatatac ttctttagcg caaggggtag 
     3481 gatcgatcca cttgtatatt tggatgaatt tttgaggaat tctgaaccag tcctaaaacg 
     3541 agtaaatagg accggcaatt cttcaagcaa caaacaggaa taccaattat taaaagataa 
     3601 cttagtcaga tcgtacaaca aggctttgaa gaaaaatgcg ccttatccaa tctttgctat 
     3661 aaagaatggc ccaaaatctc acattggaag acatttgatg acctcatttc tgtcaatgaa 
     3721 gggcctaacg gagttgacta atgttgtggg aaattggagc gataagcgtg cttctgccgt 
     3781 ggccaggaca acgtatactc atcagataac agcaatacct gatcactact tcgcactagt 
     3841 ttctcggtac tatgcatatg atccaatatc aaaggaaatg atagcattga aggatgagac 
     3901 taatccaatt gaggagtggc agcatataga acagctaaag ggtagtgctg aaggaagcat 
     3961 acgatacccc gcatggaatg ggataatatc acaggaggta ctagactacc tttcatccta 
     4021 cataaataga cgcataaagc ttggaagcgg agctactaac ttcagcctgc tgaagcaggc 
     4081 tggagacgtg gaggagaacc ctggacctcc cgggccaatg gtgagcaagg gcgaggaggt 
     4141 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt 
     4201 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 
     4261 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta 
     4321 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt 
     4381 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt 
     4441 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac 
     4501 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac 
     4561 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct 
     4621 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt 
     4681 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga 
     4741 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca 
     4801 tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat 
     4861 ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca 
     4921 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 
     4981 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt 
     5041 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct 
     5101 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt 
     5161 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg 
     5221 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 
     5281 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct 
     5341 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa 
     5401 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa 
     5461 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct 
     5521 gtacggcatg gacgagctgt acaagtaata agaattcgtc gacaatcaac ctctggatta 
     5581 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 
     5641 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 
     5701 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 
     5761 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 
     5821 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 
     5881 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 
     5941 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg 
     6001 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 
     6061 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 
     6121 gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca 
     6181 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc 
     6241 actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 
     6301 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 
     6361 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 
     6421 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 
     6481 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 
     6541 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 
     6601 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 
     6661 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     6721 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 
     6781 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc 
     6841 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 
     6901 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 
     6961 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 
     7021 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 
     7081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
     7141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
     7201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
     7261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     7321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     7381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     7441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     7501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     7561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     7621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     7681 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     7741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     7801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     7861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     7921 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     7981 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     8041 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     8101 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     8161 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     8221 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     8281 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 
     8341 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     8401 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     8461 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     8521 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     8581 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 
     8641 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     8701 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     8761 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     8821 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     8881 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     8941 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 
     9001 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 
     9061 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 
     9121 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 
     9181 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 
     9241 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 
     9301 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 
     9361 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 
     9421 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg 
//
