LOCUS       pCDH-EF1-Fon-copGFP        7964 bp    DNA     circular     30-NOV-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|751901359|
COMMENT     VNTDBDATE|751901359|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    4059..4649
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    5026..5157
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1804..1921
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    4721..4954
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(6500..7360)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     misc_feature    1309..1803
                     /vntifkey="21"
                     /label=env
     misc_feature    5682..6355
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     misc_feature    1949..3197
                     /vntifkey="21"
                     /label=EF-1\promoter
     misc_feature    3204..3236
                     /vntifkey="21"
                     /label=Frt
     misc_feature    complement(4014..4046)
                     /vntifkey="21"
                     /label=Inverse\Frt
     misc_feature    complement(3248..4003)
                     /vntifkey="21"
                     /label=copGFP
BASE COUNT     1906 a      1915 c      2191 g      1952 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 
     1921 tatcgatagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat 
     1981 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc 
     2041 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct 
     2101 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 
     2161 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 
     2221 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct 
     2281 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc 
     2341 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag 
     2401 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa 
     2461 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt 
     2521 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag 
     2581 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc 
     2641 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt 
     2701 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc 
     2761 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc 
     2821 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg 
     2881 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca 
     2941 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt 
     3001 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga 
     3061 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt 
     3121 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc 
     3181 aggtgtcgtg aggaattgga tccgaagttc ctattctctg aaagtatagg aacttcggcg 
     3241 cgccttagcg agatccggtg gagccgggtc cggcggtgcc gtccacggca gaattggacg 
     3301 actgagcgcg ggatctggcg aaggcaatgg gggtcttgaa ggcgtgctgg tactccacga 
     3361 tgcccagctc ggtgttgctg tgcagctcct ccacgcggcg gaaggcgaac atggggcccc 
     3421 cgttctgcag gatgctgggg tggatggcgc tcttgaagtg catgtggctg tccaccacga 
     3481 agctgtagta gccgccgtcg cgcaggctga aggtgcgggc gaagctgccc accagcacgt 
     3541 tatcgcccat ggggtgcagg tgctccacgg tggcgttgct gcggatgatc ttgtcggtga 
     3601 agatcacgct gtcctcgggg aagccggtgc ccaccacctt gaagtcgccg atcacgcggc 
     3661 cggcctcgta gcggtagctg aagctcacgt gcagcacgcc gccgtcctcg tacttctcga 
     3721 tgcgggtgtt ggtgtagccg ccgttgttga tggcgtgcag gaaggggttc tcgtagccgc 
     3781 tggggtaggt gccgaagtgg tagaagccgt agcccatcac gtggctcagc aggtaggggc 
     3841 tgaaggtcag ggcgcctttg gtgctcttca tcttgttggt catgcggccc tgcttggggg 
     3901 tgccctctcc gccgcccacc agctcgaact ccacgccgtt cagggtgccg gtgatgcggc 
     3961 actcgatctc catggcgggc aggccgctct cgtcgctctc catggtggct agcgaagttc 
     4021 ctatactttc agagaatagg aacttcgaat tcgtcgacaa tcaacctctg gattacaaaa 
     4081 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 
     4141 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 
     4201 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 
     4261 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 
     4321 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 
     4381 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 
     4441 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc 
     4501 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 
     4561 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 
     4621 ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca 
     4681 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 
     4741 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 
     4801 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
     4861 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
     4921 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 
     4981 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 
     5041 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     5101 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 
     5161 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     5221 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 
     5281 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt 
     5341 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     5401 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 
     5461 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 
     5521 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     5581 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     5641 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
     5701 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     5761 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     5821 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     5881 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     5941 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     6001 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     6061 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     6121 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     6181 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     6241 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     6301 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     6361 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     6421 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     6481 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     6541 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     6601 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     6661 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     6721 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     6781 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     6841 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     6901 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     6961 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     7021 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     7081 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     7141 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     7201 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     7261 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     7321 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     7381 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     7441 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 
     7501 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 
     7561 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
     7621 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
     7681 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
     7741 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
     7801 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
     7861 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
     7921 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg 
//
