LOCUS       pCDH-EF1-copGFP-T2A-Puro        8549 bp    DNA     circular     30-NOV-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|751901786|
COMMENT     VNTDBDATE|751902025|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|Demo User|
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COMMENT                                   "{\\i Hin}dIII (865)" "@N (@S)" 5 17.2743 
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COMMENT                                   "{\\i Hin}dIII (1448)" "@N (@S)" 5 18.9263 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (3964)" "@N (@S)" 5 7.6482 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (5437)" "@N (@S)" 5 -18.3844 
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FEATURES             Location/Qualifiers
     misc_feature    4644..5234
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    5611..5742
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1804..1922
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    5306..5539
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(7085..7945)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     misc_feature    1309..1803
                     /vntifkey="21"
                     /label=env
     misc_feature    6267..6940
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     misc_feature    1949..3197
                     /vntifkey="21"
                     /label=EF-1\promoter
     misc_feature    3207..3962
                     /vntifkey="21"
                     /label=copGFP
     misc_feature    3969..4031
                     /vntifkey="21"
                     /label=2A\Peptide
     misc_feature    4032..4631
                     /vntifkey="21"
                     /label=Puro
BASE COUNT     2032 a      2198 c      2350 g      1969 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 
     1921 tatcgatagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat 
     1981 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc 
     2041 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct 
     2101 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 
     2161 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 
     2221 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct 
     2281 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc 
     2341 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag 
     2401 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa 
     2461 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt 
     2521 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag 
     2581 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc 
     2641 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt 
     2701 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc 
     2761 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc 
     2821 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg 
     2881 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca 
     2941 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt 
     3001 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga 
     3061 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt 
     3121 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc 
     3181 aggtgtcgtg agcaattgga tccaccatgg agagcgacga gagcggcctg cccgccatgg 
     3241 agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg 
     3301 gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc 
     3361 tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca 
     3421 cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca 
     3481 acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct 
     3541 acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca 
     3601 gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca 
     3661 tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct 
     3721 actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc 
     3781 tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg 
     3841 agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc 
     3901 gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc 
     3961 gcaagcttgg cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga 
     4021 atcctggccc aatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 
     4081 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 
     4141 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 
     4201 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 
     4261 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 
     4321 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 
     4381 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 
     4441 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 
     4501 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 
     4561 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 
     4621 ccggtgcctg agaattcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga 
     4681 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 
     4741 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 
     4801 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 
     4861 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 
     4921 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 
     4981 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 
     5041 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 
     5101 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 
     5161 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 
     5221 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 
     5281 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 
     5341 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 
     5401 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 
     5461 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 
     5521 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 
     5581 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 
     5641 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     5701 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 
     5761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     5821 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     5881 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 
     5941 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     6001 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     6061 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 
     6121 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     6181 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     6241 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     6301 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     6361 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     6421 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     6481 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     6541 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     6601 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     6661 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     6721 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
     6781 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     6841 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     6901 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     6961 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     7021 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     7081 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     7141 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     7201 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     7261 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     7321 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     7381 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     7441 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     7501 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     7561 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     7621 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     7681 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     7741 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     7801 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     7861 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     7921 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     7981 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     8041 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 
     8101 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 
     8161 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 
     8221 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 
     8281 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 
     8341 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 
     8401 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 
     8461 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 
     8521 gttgtaaaac gacggccagt gccaagctg 
//
