LOCUS       pCDH-CMV-mCherry-T2A-Puro        7587 bp    DNA     circular     30-NOV-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|751901069|
COMMENT     VNTDBDATE|751901069|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCDH-CMV-mCherry-T2A-Puro|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     misc_feature    3682..4272
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV/promoter
     misc_feature    4649..4780
                     /vntifkey="21"
                     /label=SV40/EFL/poly(A)
     misc_feature    1798..1916
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    235..414
                     /vntifkey="21"
                     /label=HIV/LTR
     misc_feature    4789..4935
                     /vntifkey="21"
                     /label=SV40/Ori
     misc_feature    4344..4577
                     /vntifkey="21"
                     /label=3'dLTR
     misc_feature    complement(6123..6983)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    1922..2271
                     /vntifkey="21"
                     /label=CMV7/promoter
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     enhancer        1309..1797
                     /vntifkey="9"
                     /label=env
     misc_feature    complement(5305..5978)
                     /vntifkey="21"
                     /label=pUC/ORI
     CDS             2293..3000
                     /vntifkey="4"
                     /label=mCherry\ORF
     CDS             3070..3666
                     /vntifkey="4"
                     /label=Puro
     misc_feature    3001..3069
                     /vntifkey="21"
                     /label=2A\Peptide
BASE COUNT     1910 a      1915 c      2019 g      1743 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 
     2281 tagggatcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 
     2341 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 
     2401 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 
     2461 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 
     2521 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 
     2581 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 
     2641 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 
     2701 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 
     2761 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 
     2821 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 
     2881 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 
     2941 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 
     3001 aagcttggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat 
     3061 cctggcccaa tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 
     3121 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 
     3181 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 
     3241 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 
     3301 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 
     3361 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 
     3421 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 
     3481 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 
     3541 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 
     3601 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 
     3661 ggtgcctgag aattcgtcga caatcaacct ctggattaca aaatttgtga aagattgact 
     3721 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 
     3781 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 
     3841 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 
     3901 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 
     3961 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 
     4021 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 
     4081 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 
     4141 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 
     4201 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 
     4261 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 
     4321 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct 
     4381 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 
     4441 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 
     4501 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 
     4561 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 
     4621 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 
     4681 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     4741 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 
     4801 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 
     4861 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 
     4921 ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg 
     4981 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 
     5041 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
     5101 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 
     5161 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     5221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     5281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     5341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     5401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     5461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     5521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     5581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     5641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     5701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     5761 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     5821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     5881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     5941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     6001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     6061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     6121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     6181 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     6241 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     6301 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     6361 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     6421 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     6481 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     6541 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     6601 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     6661 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     6721 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     6781 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     6841 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     6901 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     6961 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     7021 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     7081 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 
     7141 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat 
     7201 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 
     7261 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 
     7321 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 
     7381 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg 
     7441 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 
     7501 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 
     7561 tgtaaaacga cggccagtgc caagctg 
//
