LOCUS       pCDH-CB-IRES-copGFP-T2A-Puro        8705 bp    DNA     circular      4-DEC-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|752418453|
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COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (4120)" "@N (@S)" 5 6.07361 
COMMENT                                   -36.5889 0 -10 5.59471 0.932452 #336#) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (5593)" "@N (@S)" 5 -14.4821 
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FEATURES             Location/Qualifiers
     misc_feature    4800..5390
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    5767..5898
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1798..1916
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    5462..5695
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(7241..8101)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     misc_feature    1309..1797
                     /vntifkey="21"
                     /label=env
     misc_feature    6423..7096
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     promoter        2226..2498
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=#800000
                     /vntifkey="29"
                     /label=chicken\beta\actin\promoter
     enhancer        1941..2225
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#80ffff
                     /vntifkey="9"
                     /label=CMV\promoter
     misc_feature    2765..3353
                     /vntifkey="21"
                     /label=ires
     misc_feature    3363..4118
                     /vntifkey="21"
                     /label=copGFP
     tRNA            4125..4187
                     /vntifkey="59"
                     /label=T2A
     misc_feature    4188..4787
                     /vntifkey="21"
                     /label=Puro
BASE COUNT     2123 a      2261 c      2332 g      1989 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 
     1921 ttaattaagg tacctctggt cgttacataa cttacggtaa atggcccgcc tggctgaccg 
     1981 cccaacgacc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
     2041 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 
     2101 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
     2161 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctact 
     2221 cgaggccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta 
     2281 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggggggc 
     2341 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg 
     2401 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc 
     2461 ggcggcccta taaaaagcga agcgcgcggc gggcgggagc gggatcagcc accgcggtgg 
     2521 cggccctaga gtcgatcgag gaactgaaaa accagaaagt taactggtaa gtttagtctt 
     2581 tttgtctttt atttcaggtc cccgatccgg tggtggtgca aatcaaagaa ctgctcctca 
     2641 gtggatgttg cctttacttc taggcctgta cggaagtgtt acttctgctc taaaagctgc 
     2701 ggaattgtac ccgcggccga tccaccggtc gggatccgct agcgtttaaa cgcggccgca 
     2761 attgatccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata 
     2821 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt 
     2881 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct 
     2941 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc 
     3001 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga 
     3061 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc 
     3121 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt 
     3181 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg 
     3241 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc 
     3301 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaagatcca 
     3361 ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca 
     3421 ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc 
     3481 gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga 
     3541 gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc 
     3601 ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg 
     3661 acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg 
     3721 acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca 
     3781 tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca 
     3841 gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc 
     3901 acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg 
     3961 ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc 
     4021 acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg 
     4081 tggacggcac cgccggaccc ggctccaccg gatctcgcaa gcttggcagt ggagagggca 
     4141 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg accgagtaca 
     4201 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg 
     4261 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc 
     4321 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 
     4381 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 
     4441 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 
     4501 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 
     4561 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 
     4621 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc 
     4681 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc 
     4741 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagaa ttcgtcgaca 
     4801 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 
     4861 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 
     4921 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 
     4981 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 
     5041 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 
     5101 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 
     5161 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 
     5221 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 
     5281 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 
     5341 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta 
     5401 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 
     5461 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 
     5521 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
     5581 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
     5641 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 
     5701 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 
     5761 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     5821 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     5881 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 
     5941 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 
     6001 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 
     6061 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 
     6121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 
     6181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 
     6241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 
     6301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     6361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     6421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     6481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     6541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     6601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     6661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     6721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     6781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     6841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     6901 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 
     6961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     7021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     7081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     7141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     7201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
     7261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     7321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     7381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     7441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     7501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     7561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     7621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     7681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     7741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     7801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     7861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     7921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     7981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     8041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     8101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     8161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     8221 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 
     8281 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 
     8341 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 
     8401 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 
     8461 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 
     8521 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 
     8581 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 
     8641 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 
     8701 agctg 
//
