LOCUS pDsRed_TSTEP_11302015 6591 bp DNA circular 30-NOV-2015 FEATURES Location/Qualifiers primer_bind 624..642 /note="T7" primer_bind complement(4402..4421) /note="T3" primer_bind complement(4438..4456) /note="M13 rev " primer_bind 600..616 /note="M13 fwd" misc_feature complement(678..710) /note="Rippase recognition sequence (plus GT)" gene 711..1442 /note="TagRFP-T " misc_feature 1481..2146 /note="p10 terminator" misc_feature complement(2268..2301) /label=LoxP gene 2533..3210 /label=DsRed_Express_2 /gene="DsRed_Express_2" /translation="MADYDLESRPLQEQVVAALGALVLLHDGVVLVVGGDVQLGVHVV VAGQLHGLLGHVDGLELHQVVAAVLQLQGLVDLALQHAVAGVQALGGRLPAHSLLLHY GAVGGEVHADELHLVDEGAVLEGGVLGHGHHAAVLEVHHALPLEALGEGQLLVVGDVG GVLHVHLGAVLELGGQDVPGEGQGAALGHLQLGGLGALVGAALALALDLELVAVHGAL HAHLEAHELLDDVLGGGHGGDRLRADCLACSAALVYLLSFRLATCSLCLFELNCRSVD EAPLFILRRSRVRNR*" misc_feature complement(3459..3492) /label=LoxP /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDG GVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGE IHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAE GRHHLFL*" misc_feature 3598..3621 /dnas_title="PreScission protease recognitions site" /note="Prescission protease recognitions site" /note="" gene 3640..4359 /note="EGFP" misc_feature complement(3553..3583) /note="2nd Rippase recognition sequence" misc_feature 135..441 /note="f1" misc_feature 661..672 /note="5' MCS" misc_feature 4358..4389 /note="3' MCS" misc_feature complement(5603..6463) /note="amp" BASE COUNT 1732 a 1730 c 1658 g 1471 t ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg aGGCACCGGT 661 GATATCAGGC CTATGCATTG ATGAAAGAAT ACGTTATTCT TTCATCAAgt ATGGTGTCTA 721 AGGGCGAAGA GCTGATTAAG GAGAACATGC ACATGAAGCT GTACATGGAG GGCACCGTGA 781 ACAACCACCA CTTCAAGTGC ACATCCGAGG GCGAAGGCAA GCCCTACGAG GGCACCCAGA 841 CCATGAGAAT CAAGGTGGTC GAGGGCGGCC CTCTCCCCTT CGCCTTCGAC ATCCTGGCTA 901 CCAGCTTCAT GTACGGCAGC AGAACCTTCA TCAACCACAC CCAGGGCATC CCCGATTTCT 961 TTAAGCAGTC CTTCCCTGAG GGCTTCACAT GGGAGAGAGT CACCACATAC GAAGACGGGG 1021 GCGTGCTGAC CGCTACCCAG GACACCAGCC TCCAGGACGG CTGCCTCATC TACAACGTCA 1081 AGATCAGAGG GGTGAACTTC CCATCCAACG GCCCTGTGAT GCAGAAGAAA ACACTCGGCT 1141 GGGAGGCCAA CACCGAGATG CTGTACCCCG CTGACGGCGG CCTGGAAGGC AGAACCGACA 1201 TGGCCCTGAA GCTCGTGGGC GGGGGCCACC TGATCTGCAA CTTCAAGACC ACATACAGAT 1261 CCAAGAAACC CGCTAAGAAC CTCAAGATGC CCGGCGTCTA CTATGTGGAC CACAGACTGG 1321 AAAGAATCAA GGAGGCCGAC AAAGAGACCT ACGTCGAGCA GCACGAGGTG GCTGTGGCCA 1381 GATACTGCGA CCTCCCTAGC AAACTGGGGC ACAAACTTAA TGGCATGGAC GAGCTGTACA 1441 AGtAGAAGGG CGAATTCGCG AGCTCTACAA GTAATCTAGA ATGAATCGTT TTTAAAATAA 1501 CAAATCAATT GTTTTATAAT ATTCGTACGA TTCTTTGATT ATGTAATAAA ATGTGATCAT 1561 TAGGAAGATT ACGAAAAATA TAAAAAATAT GAGTTCTGTG TGTATAACAA ATGCTGTAAA 1621 CGCCACAATT GTGTTTGTTG CAAATAAACC CATGATTATT TGATTAAAAT TGTTGTTTTC 1681 TTTGTTCATA GACAATAGTG TGTTTTGCCT AAACGTGTAC TGCATAAACT CCATGCGAGT 1741 GTATAGCGAG CTAGTGGCTA ACGCTTGCCC CACCAAAGTA GATTCGTCAA AATCCTCAAT 1801 TTCATCACCC TCCTCCAAGT TTAACATTTG GCCGTCGGAA TTAACTTCTA AAGATGCCAC 1861 ATAATCTAAT AAATGAAATA GAGATTCAAA CGTGGCGTCA TCGTCCGTTT CGACCATTTC 1921 CGAAAAGAAC TCGGGCATAA ACTCTATGAT TTCTCTGGAC GTGGTGTTGT CGAAACTCTC 1981 AAAGTACGCA GTCAGGAACG TGCGCGACAT GTCGTCGGGA AACTCGCGCG GAAACATGTT 2041 GTTGTAACCG AACGGGTCCC ATAGCGCCAA AACCAAATCT GCCAGCGTCA ATAGAATGAG 2101 CACGATGCCG ACAATGGAGC TGGCTTGGAT AGCGATTCGA GTTAACGGCC gcgcggacat 2161 atgcaCACCT GCgatcgtag tgccccaact ggggcggaca tatgcaCACC TGCgatcgta 2221 gtgccccaac tggggtaacc tttgagttct ctcagttggg ggcgtagata acttcgtata 2281 atgtatgcta tacgaagtta tcgtacgGGA TCTAATTCAA TTAGAGACTA ATTCAATTAG 2341 AGCTAATTCA ATTAGGATCC AAGCTTATCG ATTTCGAACC CTCGACCGCC GGAGTATAAA 2401 TAGAGGCGCT TCGTCTACGG AGCGACAATT CAATTCAAAC AAGCAAAGTG AACACGTCGC 2461 TAAGCGAAAG CTAAGCAAAT AAACAAGCGC AGCTGAACAA GCTAAACAAT CGGCTCGAAG 2521 CCGGTCGCCA CCatgGCCTC CTCCGAGGAC GTCATCAAGG AGTTCATGCG CTTCAAGGTG 2581 CGCATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC 2641 CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGCGGCCC CCTGCCCTTC 2701 GCCTGGGACA TCCTGTCCCC CCAGTTCCAG TACGGCTCCA AGGTGTACGT GAAGCACCCC 2761 GCCGACATCC CCGACTACAA GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG 2821 ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT cCAGGACGGC 2881 TCCTTCATCT ACAAGGTGAA GTTCATCGGC GTGAACTTCC CCTCCGACGG CCCCGTAATG 2941 CAGAAGAAGA CTATGGGCTG GGAGGCgTCC ACCGAGCGCC TGTACCCCCG CGACGGCGTG 3001 CTGAAGGGCG AGATCCACAA GGCCCTGAAG CTGAAGGACG GCGGCCACTA CCTGGTGGAG 3061 TTCAAGTCCA TCTACATGGC CAAGAAGCCC GTGCAGCTGC CCGGCTACTA CTACGTGGAC 3121 TCCAAGCTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAGCA GTACGAGCGC 3181 GCCGAGGGCC GCCACCACCT GTTCCTGTAG gggccGCGAC TCTAGATCAT AATCAGCCAT 3241 ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG 3301 AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC 3361 aaataaaGCA ATAGCATCAC AAATTTCACa aataaaGCAT TTTTTTCACT GCATTCTAGT 3421 TGTGGTTTGT CCAAACTCAT CAATGTATCT TAaccggtat aacttcgtat aatgtatgct 3481 atacgaagtt ataGAAGAGC actagtaaag atctccatgc taaagatctc catgcataag 3541 gcgcgcctag gcTTGATGAA AGAATACGTT ATTCTTTCAT CAAgctctgg atcgtccTTG 3601 GAGGTCCTGT TCCAGGGCCC Cggatccggt TCAGGATCAA TGGTGAGCAA GGGCGAGGAG 3661 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG 3721 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC 3781 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC 3841 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC 3901 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC 3961 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG 4021 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC 4081 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG 4141 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC 4201 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC 4261 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC 4321 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAGc gctCGTTTAA ACCCTTTCGA 4381 AGGACGCGTc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat 4441 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 4501 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 4681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 4741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 4801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 4861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 4921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 4981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 5041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 5101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 5161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 5221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 5281 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 5341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 5401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 5461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 5521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 5581 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 5641 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 5701 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 5761 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 5821 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 5881 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 5941 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 6001 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 6061 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 6121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 6181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 6241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 6301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 6361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 6421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 6481 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 6541 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c //