LOCUS       pCDH-EF1S        6421 bp    DNA     circular     13-JUN-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|734457683|
COMMENT     VNTDBDATE|736596374|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCDH-EF1S|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
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FEATURES             Location/Qualifiers
     misc_feature    2516..3106
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV/promoter
     misc_feature    3483..3614
                     /vntifkey="21"
                     /label=SV40/EFL/poly(A)
     misc_feature    1798..1916
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    235..414
                     /vntifkey="21"
                     /label=HIV/LTR
     misc_feature    3623..3769
                     /vntifkey="21"
                     /label=SV40/Ori
     misc_feature    3178..3411
                     /vntifkey="21"
                     /label=3'dLTR
     misc_feature    complement(4957..5817)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     enhancer        1309..1797
                     /vntifkey="9"
                     /label=env
     misc_feature    complement(4139..4812)
                     /vntifkey="21"
                     /label=pUC/ORI
     misc_feature    1922..2475
                     /vntifkey="21"
                     /label=EF-1\Promoter
BASE COUNT     1647 a      1534 c      1650 g      1590 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 
     1921 tcaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 
     1981 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 
     2041 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 
     2101 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 
     2161 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 
     2221 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 
     2281 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 
     2341 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 
     2401 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 
     2461 gcgcctacgc tagacggatc cgctagcgtt taaacgcggc cgcgaattcg tcgacaatca 
     2521 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 
     2581 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 
     2641 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 
     2701 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 
     2761 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 
     2821 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 
     2881 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 
     2941 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 
     3001 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 
     3061 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac 
     3121 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 
     3181 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct 
     3241 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 
     3301 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 
     3361 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 
     3421 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 
     3481 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 
     3541 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 
     3601 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc 
     3661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     3721 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc 
     3781 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 
     3841 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 
     3901 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 
     3961 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 
     4021 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     4081 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
     4141 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 
     4201 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     4261 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     4321 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     4381 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     4441 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     4501 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     4561 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     4621 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     4681 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 
     4741 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 
     4801 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 
     4861 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 
     4921 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 
     4981 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 
     5041 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 
     5101 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 
     5161 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 
     5221 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 
     5281 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 
     5341 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 
     5401 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 
     5461 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 
     5521 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 
     5581 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 
     5641 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 
     5701 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 
     5761 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 
     5821 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 
     5881 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 
     5941 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 
     6001 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 
     6061 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 
     6121 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 
     6181 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     6241 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 
     6301 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 
     6361 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 
     6421 g 
//
