LOCUS       pCDH-EF1-Luc2-P2A-tdTomato       10289 bp    DNA     circular      8-DEC-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|752765168|
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COMMENT                                   "{\\i Hin}dIII (1448)" "@N (@S)" 5 14.8767 0 
COMMENT                                   0 -10 5.54476 0.932452 #335#) 
COMMENT                             #337=(CScratch 
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COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (4858)" "@N (@S)" 5 6.12305 
COMMENT                                   -22.6503 0 -10 5.59471 0.932452 #337#) 
COMMENT                             #339=(CScratch 
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COMMENT                             #340=(CLabel (CWidget 0 (0 0) 1 2 0 0 #187# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (7177)" "@N (@S)" 5 -14.5518 
COMMENT                                   3.48466 0 -10 5.72792 0.932452 #339#)) 
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COMMENT               #341=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1381 100) 
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COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2081696188 
COMMENT       (CStringList) 0 2081694076 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    6384..6974
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    7351..7482
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1804..1921
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    7046..7279
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(8825..9685)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     misc_feature    1309..1803
                     /vntifkey="21"
                     /label=env
     misc_feature    8007..8680
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     misc_feature    1949..3197
                     /vntifkey="21"
                     /label=EF-1\promoter
     misc_feature    7491..7638
                     /vntifkey="21"
                     /label=SV40\ORI
     misc_feature    3207..4856
                     /vntifkey="21"
                     /label=Luc2
     misc_feature    4863..4928
                     /vntifkey="21"
                     /label=P2A
     misc_feature    4938..6365
                     /vntifkey="21"
                     /label=tdTomato
BASE COUNT     2503 a      2675 c      2818 g      2293 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 
     1921 tatcgatagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat 
     1981 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc 
     2041 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct 
     2101 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 
     2161 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 
     2221 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct 
     2281 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc 
     2341 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag 
     2401 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa 
     2461 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt 
     2521 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag 
     2581 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc 
     2641 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt 
     2701 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc 
     2761 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc 
     2821 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg 
     2881 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca 
     2941 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt 
     3001 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga 
     3061 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt 
     3121 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc 
     3181 aggtgtcgtg agcaattgga tccaccatgg aagatgccaa aaacattaag aagggcccag 
     3241 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc 
     3301 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta 
     3361 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc 
     3421 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg 
     3481 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc 
     3541 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag 
     3601 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca 
     3661 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt 
     3721 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca 
     3781 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc 
     3841 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca 
     3901 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca 
     3961 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc 
     4021 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat 
     4081 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga 
     4141 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc 
     4201 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca 
     4261 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta 
     4321 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg 
     4381 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca 
     4441 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact 
     4501 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag 
     4561 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc 
     4621 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa 
     4681 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc 
     4741 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg 
     4801 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgaagc 
     4861 ttggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc 
     4921 ctggacctcc cgggccaatg gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct 
     4981 tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg 
     5041 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc 
     5101 tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga 
     5161 agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg 
     5221 agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc 
     5281 aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc 
     5341 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg 
     5401 acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc 
     5461 tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact 
     5521 acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt 
     5581 acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc agcaccggca 
     5641 gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc aaagagttca 
     5701 tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg 
     5761 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg 
     5821 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt 
     5881 acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca 
     5941 agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct 
     6001 ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg 
     6061 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc 
     6121 cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc 
     6181 actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct 
     6241 actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg 
     6301 aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg gacgagctgt 
     6361 acaagtaata agaattcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga 
     6421 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 
     6481 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 
     6541 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 
     6601 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 
     6661 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 
     6721 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 
     6781 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 
     6841 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 
     6901 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 
     6961 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 
     7021 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 
     7081 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 
     7141 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 
     7201 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 
     7261 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 
     7321 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 
     7381 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     7441 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 
     7501 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     7561 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     7621 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 
     7681 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     7741 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     7801 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 
     7861 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     7921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     7981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     8041 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     8101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     8161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     8221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     8281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     8341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     8401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     8461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
     8521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     8581 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     8641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     8701 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     8761 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     8821 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     8881 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     8941 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     9001 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     9061 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     9121 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     9181 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     9241 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     9301 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     9361 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     9421 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     9481 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     9541 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     9601 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     9661 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     9721 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     9781 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 
     9841 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 
     9901 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 
     9961 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 
    10021 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 
    10081 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 
    10141 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 
    10201 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 
    10261 gttgtaaaac gacggccagt gccaagctg 
//
