LOCUS       pLL3.7-mTNFalpha-shRNA3        7692 bp    DNA     circular      8-DEC-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|752768604|
COMMENT     VNTDBDATE|752768766|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLL3.7-mTNFalpha-shRNA3|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Kazuhiro Oka|
COMMENT     VNTOAUTHORTEL|713-798-7381|
COMMENT     VNTOAUTHOREML|kazuhiro@bcm.edu|
COMMENT     VNTOAUTHORAD1|Baylor College of Medicine|
COMMENT     VNTOAUTHORAD2|One Baylor Plaza|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
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FEATURES             Location/Qualifiers
     misc_feature    239..815
                     /vntifkey="21"
                     /label=CAG\enhancer/CMV\promoter
     misc_feature    835..1015
                     /vntifkey="21"
                     /label=HIV_5'-LTR/truncated\3'-LTR
     misc_feature    1126..1170
                     /vntifkey="21"
                     /label=psi_pack
     misc_feature    1686..1919
                     /vntifkey="21"
                     /label=RRE
     misc_feature    2450..2465
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    2613..2935
                     /vntifkey="21"
                     /label=U6\promoter
     misc_feature    3052..3085
                     /vntifkey="21"
                     /label=loxP
     misc_feature    3205..3729
                     /vntifkey="21"
                     /label=CAG\enhancer/CMV\promoter
     misc_feature    3753..4469
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    4583..5170
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    4492..4525
                     /vntifkey="21"
                     /label=loxP
     misc_feature    5361..5376
                     /vntifkey="21"
                     /label=cPPT/U3PPT
     misc_feature    5698..5878
                     /vntifkey="21"
                     /label=HIV_5'-LTR/truncated\3'-LTR
     misc_feature    complement(5925..6544)
                     /vntifkey="21"
                     /label=pBR322\origin
     misc_feature    complement(6699..7559)
                     /vntifkey="21"
                     /label=Amp\r
     misc_feature    2936..2992
                     /vntifkey="21"
                     /label=mTNFalpha-sh3
BASE COUNT     2041 a      1868 c      1925 g      1858 t 
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga 
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca 
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg 
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag agatccgacg ccgccatctc 
     2641 taggcccgcg ccggccccct cgcacagact tgtgggagaa gctcggctac tcccctgccc 
     2701 cggttaattt gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac 
     2761 agataatctg ttctttttaa tactagctac attttacatg ataggcttgg atttctataa 
     2821 gagatacaaa tactaaatta ttattttaaa aaacagcaca aaaggaaact caccctaact 
     2881 gtaaagtaat tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgtttgcct 
     2941 ccctctcatc agttctatct cgagatagaa ctgatgagag ggaggttttt tctcgaggtc 
     3001 gacggtatcg ataagctcgc ttcacgagat tccagcaggt cgagggacct aataacttcg 
     3061 tatagcatac attatacgaa gttatattaa gggttccaag cttaagcggc cgcgtggata 
     3121 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat 
     3181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 
     3241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 
     3301 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 
     3361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 
     3421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 
     3481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 
     3541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 
     3601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 
     3661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac 
     3721 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca 
     3781 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 
     3841 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 
     3901 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 
     3961 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 
     4021 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 
     4081 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 
     4141 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 
     4201 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 
     4261 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 
     4321 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 
     4381 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 
     4441 tcactctcgg catggacgag ctgtacaagt aggaattcgt cgagggacct aataacttcg 
     4501 tatagcatac attatacgaa gttatacatg tttaagggtt ccggttccac taggtacaat 
     4561 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg 
     4621 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 
     4681 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 
     4741 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 
     4801 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 
     4861 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 
     4921 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat 
     4981 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 
     5041 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 
     5101 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 
     5161 cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca 
     5221 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag 
     5281 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct 
     5341 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 
     5401 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg 
     5461 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag 
     5521 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg 
     5581 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg 
     5641 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg 
     5701 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 
     5761 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 
     5821 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc 
     5881 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     5941 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     6001 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     6061 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     6121 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     6181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     6241 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     6301 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     6361 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     6421 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     6481 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     6541 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     6601 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     6661 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     6721 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     6781 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     6841 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     6901 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     6961 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     7021 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     7081 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     7141 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     7201 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     7261 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     7321 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     7381 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     7441 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     7501 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     7561 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     7621 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     7681 gtgccacctg ac 
//
