LOCUS       H2B-GFP	5107        5885 bp    DNA     circular     19-MAR-1980
DEFINITION  H2B-GFP.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|H2B-GFP	5107|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 630 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2554 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 671 1 1365 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 1245 0 4899 1 0 "") 
COMMENT        #4=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 0 0 "") 
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COMMENT             0 5693 0 0 "") 
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COMMENT        #16=(CFSignal (CObList) "CMV_promoter" 30 0 0 520 589 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "520..589") 
COMMENT        #17=(CFSignal (CObList) "EBV_rev_primer" 21 0 0 2764 2783 0 
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COMMENT        #25=(CFSignal (CObList) "ORF frame 3" 4 0 0 3781 4575 0 (CStringList) 
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COMMENT        #26=(CFSignal (CObList) "ORF frame 3" 4 0 1 4090 4626 0 (CStringList) 
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COMMENT        #27=(CFSignal (CObList) "TK_PA_terminator" 43 0 0 4750 5019 0 
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COMMENT        #28=(CFSignal (CObList) "pBR322_origin" 33 0 0 5167 5786 0 (CStringList) 
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COMMENT        #29=(CFSignal (CObList) "Kozag" 20 0 0 633 641 0 (CStringList) 
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COMMENT        #30=(CFSignal (CObList) "Start codon" 21 0 0 642 644 0 (CStringList) 
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COMMENT        #31=(CFSignal (CObList) "mCherry" 4 0 0 1365 2066 0 (CStringList) 
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COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                          "Modification Date in the Original DB: 19-MAR-1980" 1) 
COMMENT                     #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
COMMENT                          1) 
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COMMENT                          "Associated Genes:  NeoR/KanR" 1))) "Standard Fields") 
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COMMENT                          "Misc. Binding Site (2 total)") 
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FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="H2B-GFP"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        10..562
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    65..352
                     /vntifkey="21"
                     /label=CAG_enhancer
     misc_feature    519..539
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        520..589
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    2764..2783
                     /vntifkey="21"
                     /label=EBV_rev_primer
     rep_origin      complement(2933..3239)
                     /vntifkey="33"
                     /label=f1_origin
     promoter        3318..3346
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(3412..3432)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(3418..3633)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        3430..3698
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      3597..3674
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    3659..3678
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             3781..4575
                     /vntifkey="4"
                     /label=ORF\frame\3
     gene            3784..4572
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     CDS             complement(4090..4626)
                     /vntifkey="4"
                     /label=ORF\frame\3
     terminator      4750..5019
                     /vntifkey="43"
                     /label=TK_PA_terminator
     rep_origin      5167..5786
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_binding    633..641
                     /vntifkey="20"
                     /label=Kozag
     misc_feature    642..644
                     /vntifkey="21"
                     /label=Start\codon
     CDS             1365..2066
                     /vntifkey="4"
                     /label=mCherry
     CDS             2097..2504
                     /vntifkey="4"
                     /label=AsLOV2
     CDS             2506..2543
                     /vntifkey="4"
                     /label=NES\21
     CDS             683..1356
                     /vntifkey="4"
                     /label=ccdB
     misc_binding    1389..2543
                     /vntifkey="20"
                     /label=BbsI\mutated
BASE COUNT     1468 a      1499 c      1574 g      1344 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attccgccac catggccgca gcaaagcgcg 
      661 tgaagctgga catgaagtct tccgcgtgga tccggcttac taaaagccag ataacagtat 
      721 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt 
      781 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct 
      841 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca 
      901 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc 
      961 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg 
     1021 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg 
     1081 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac 
     1141 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa 
     1201 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag 
     1261 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg 
     1321 gaatataaat gtcaggctcc cttatacaca gccagtgaag acaagtgagc aagggcgagg 
     1381 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg 
     1441 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc 
     1501 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt 
     1561 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact 
     1621 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg 
     1681 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg 
     1741 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aaaaccatgg 
     1801 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca 
     1861 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca 
     1921 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca 
     1981 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact 
     2041 ccaccggcgg catggacgag ctgtacaagg gtggatctgg aggttcaggt ggaagtttgg 
     2101 ctactacact tgaacgtatt gagaagaact ttgtcattac tgacccaaga ttgccagata 
     2161 atcccattat attcgcgtcc gatagtttct tgcagttgac agaatatagc cgtgaagaaa 
     2221 ttttgggaag aaactgcagg tttctacaag gtcctgaaac tgatcgcgcg acagtgagaa 
     2281 aaattagaga tgccatagat aaccaaacag aggtcactgt tcagctgatt aattatacaa 
     2341 agagtggtaa aaagttctgg aacctctttc acttgcagcc tatgcgagat cagaagggag 
     2401 atgtccagta ctttattggg gttcagttgg atggaactga gcatgtccga gatgctgccg 
     2461 agagagaggg agtcatgctg attaagaaaa ctgcagaaaa tattgacgag ctgctgaagg 
     2521 agctggccga cctgaacctg gactagctcg agcggccgcg actctagatc ataatcagcc 
     2581 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 
     2641 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 
     2701 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     2761 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt 
     2821 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
     2881 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
     2941 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
     3001 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
     3061 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
     3121 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
     3181 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
     3241 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     3301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     3361 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt 
     3421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 
     3481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 
     3541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 
     3601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     3661 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 
     3721 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc 
     3781 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 
     3841 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 
     3901 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 
     3961 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 
     4021 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 
     4081 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 
     4141 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 
     4201 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 
     4261 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 
     4321 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 
     4381 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 
     4441 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 
     4501 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 
     4561 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 
     4621 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 
     4681 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 
     4741 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 
     4801 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac 
     4861 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc 
     4921 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 
     4981 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac 
     5041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
     5101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     5161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     5221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     5281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 
     5341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     5401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     5461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     5521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     5581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     5641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     5701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     5761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     5821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca 
     5881 tgcat 
//
