LOCUS       H2B-GFP	5107        5585 bp    DNA     circular     19-MAR-1980
DEFINITION  H2B-GFP.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|H2B-GFP	5107|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="H2B-GFP"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        10..562
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    65..352
                     /vntifkey="21"
                     /label=CAG_enhancer
     CDS             complement(447..905)
                     /vntifkey="4"
                     /label=ORF\frame\3
     misc_feature    519..539
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        520..589
                     /vntifkey="30"
                     /label=CMV_promoter
     CDS             657..1767
                     /vntifkey="4"
                     /label=ORF\frame\3
     gene            1056..1767
                     /gene="EGFP"
                     /vntifkey="60"
     misc_feature    complement(1098..1119)
                     /vntifkey="21"
                     /label=EGFP_N_primer
     misc_feature    1236..1265
                     /vntifkey="21"
                     /label=Y66\(EGFP)
     misc_feature    1706..1727
                     /vntifkey="21"
                     /label=EGFP_C_primer
     misc_feature    2464..2483
                     /vntifkey="21"
                     /label=EBV_rev_primer
     rep_origin      complement(2633..2939)
                     /vntifkey="33"
                     /label=f1_origin
     promoter        3018..3046
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(3112..3132)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(3118..3333)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        3130..3398
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      3297..3374
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    3359..3378
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             3481..4275
                     /vntifkey="4"
                     /label=ORF\frame\3
     gene            3484..4272
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     CDS             complement(3790..4326)
                     /vntifkey="4"
                     /label=ORF\frame\3
     terminator      4450..4719
                     /vntifkey="43"
                     /label=TK_PA_terminator
     rep_origin      4867..5486
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             1056..1767
                     /vntifkey="4"
                     /label=EGFP
     CDS             657..1052
                     /vntifkey="4"
                     /label=H2B
     misc_feature    1770..1796
                     /vntifkey="21"
                     /label=GS\linker
     CDS             1797..2204
                     /vntifkey="4"
                     /label=AsLOV2
     misc_feature    2205..2243
                     /vntifkey="21"
                     /label=NES\27
BASE COUNT     1378 a      1484 c      1480 g      1243 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gccaccatgc 
      661 cagagccagc gaagtctgct cccgccccga aaaagggctc caagaaggcg gtgactaagg 
      721 cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa ggagagctat tccatctatg 
      781 tgtacaaggt tctgaagcag gtccaccctg acaccggcat ttcgtccaag gccatgggca 
      841 tcatgaattc gtttgtgaac gacattttcg agcgcatcgc aggtgaggct tcccgcctgg 
      901 cgcattacaa caagcgctcg accatcacct ccagggagat ccagacggcc gtgcgcctgc 
      961 tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg tactaaggcc atcaccaagt 
     1021 acaccagcgc taaggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca 
     1081 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 
     1141 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 
     1201 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 
     1261 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 
     1321 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 
     1381 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 
     1441 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 
     1501 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 
     1561 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 
     1621 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 
     1681 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 
     1741 tcactctcgg catggacgag ctgtacaagg gtggatctgg aggttcaggt ggaagtttgg 
     1801 ctactacact tgaacgtatt gagaagaact ttgtcattac tgacccaaga ttgccagata 
     1861 atcccattat attcgcgtcc gatagtttct tgcagttgac agaatatagc cgtgaagaaa 
     1921 ttttgggaag aaactgcagg tttctacaag gtcctgaaac tgatcgcgcg acagtgagaa 
     1981 aaattagaga tgccatagat aaccaaacag aggtcactgt tcagctgatt aattatacaa 
     2041 agagtggtaa aaagttctgg aacctctttc acttgcagcc tatgcgagat cagaagggag 
     2101 atgtccagta ctttattggg gttcagttgg atggaactga gcatgtccga gatgctgccg 
     2161 agagagaggg agtcatgctg attaagaaaa ctgcagaaaa tattgaggag ctggccaagg 
     2221 agctggccga cctgaacctg gactagctcg agcggccgcg actctagatc ataatcagcc 
     2281 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 
     2341 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 
     2401 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     2461 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt 
     2521 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
     2581 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
     2641 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
     2701 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
     2761 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
     2821 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
     2881 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
     2941 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     3001 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     3061 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt 
     3121 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 
     3181 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 
     3241 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 
     3301 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     3361 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 
     3421 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc 
     3481 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 
     3541 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 
     3601 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 
     3661 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 
     3721 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 
     3781 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 
     3841 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 
     3901 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 
     3961 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 
     4021 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 
     4081 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 
     4141 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 
     4201 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 
     4261 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 
     4321 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 
     4381 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 
     4441 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 
     4501 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac 
     4561 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc 
     4621 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 
     4681 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac 
     4741 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
     4801 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     4861 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     4921 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     4981 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 
     5041 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     5101 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     5161 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     5221 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     5281 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     5341 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     5401 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     5461 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     5521 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca 
     5581 tgcat 
//
