LOCUS       Exported                2516 bp ds-DNA     circular SYN 07-APR-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pTU1-C-lacZ
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Karen Polizzi's Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 21, 2016 from SnapGene 3.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    35..51
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     59..75
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    453..458
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     rep_origin      complement(635..1223)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1394..2254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2255..2359)
                     /gene="bla"
                     /note="AmpR promoter"
     terminator      2449..2492
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    2495..2514
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaccg gctatagaga cctctagaca tatgttgtga gcggataaca atttcacaca
       61 ggaaacagct atgaccatgg tacaaagagg agaaaggaca tgggtgaccc gaaactgctg
      121 ctggaagttc cgcacgctat ctcttcttct ccgggtaact ctctggctgt tgttctgcaa
      181 cgtcgtgact gggaaaaccc gggtgttacc cagctgaacc gtctggctgc tcacccgccg
      241 ttcgcttctt ggcgtaactc tgaagaagct cgtaccgacc gtccgtccca gcagctgcgt
      301 tctctgatcc gtctgctgac caaaccggaa cgtaaactgt cttggctgct gccgccgctg
      361 tctaacaact aacgcaaaaa accccgcccc tgacagggcg gggttttttc gcactagtgg
      421 atccgcatgc ggtctcatgt tgaagagaga cgctgcaggc ttcctcgctc actgactcgc
      481 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      541 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      601 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      661 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      781 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      841 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      901 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      961 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1021 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     1081 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1141 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1501 taccatctgg ccccagtgct gcaatgatac cgcgagagcc acgctcaccg gctccagatt
     1561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     2401 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
     2461 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagaa
//
