LOCUS       Exported                2506 bp ds-DNA     circular SYN 07-APR-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pTU2-D-lacZ
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Karen Polizzi's Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 21, 2016 from SnapGene 3.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..22
                     /note="GG2D"
     protein_bind    35..51
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     59..75
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    431..452
                     /note="GG2D"
     misc_feature    458
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      459..516
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(711..1299)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1482..1576
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(1597..2256)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      2440..2483
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    2486..2501
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcatcg aatctagaga cgtctagaca tatgttgtga gcggataaca atttcacaca
       61 ggaaacagct atgaccatgg tacaaagagg agaaaggaca tgggtgaccc gaaactgctg
      121 ctggaagttc cgcacgctat ctcttcttct ccgggtaact ctctggctgt tgttctgcaa
      181 cgtcgtgact gggaaaaccc gggtgttacc cagctgaacc gtctggctgc tcacccgccg
      241 ttcgcttctt ggcgtaactc tgaagaagct cgtaccgacc gtccgtccca gcagctgcgt
      301 tctctgatcc gtctgctgac caaaccggaa cgtaaactgt cttggctgct gccgccgctg
      361 tctaacaact aacgcaaaaa accccgcccc tgacagggcg gggttttttc gcactagtgg
      421 atccgcatgc cgtctcatta gtgttagaga ccctgcagtc cggcaaaaaa gggcaaggtg
      481 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
      541 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
      721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1141 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1441 agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac
     1501 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1561 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     1621 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1681 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1741 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1801 actcacccag ggattggctg acacgaaaaa catattctca ataaaccctt tagggaaata
     1861 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     1921 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     1981 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     2041 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     2101 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     2161 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     2221 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     2281 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     2341 acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca
     2401 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct
     2461 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctaga
//
