LOCUS       pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro        9134 bp    DNA     circular      8-DEC-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|752772719|
COMMENT     VNTDBDATE|752772719|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     misc_feature    5229..5819
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    6196..6327
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1804..1921
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    5891..6124
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(7670..8530)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     misc_feature    1309..1803
                     /vntifkey="21"
                     /label=env
     misc_feature    6852..7525
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     misc_feature    1949..3197
                     /vntifkey="21"
                     /label=EF-1\promoter
     misc_feature    6336..6483
                     /vntifkey="21"
                     /label=SV40\ORI
     misc_feature    3204..3725
                     /vntifkey="21"
                     /label=Nluc
     misc_feature    3207..3719
                     /vntifkey="21"
                     /label=Nluc_ORF
     CDS             4617..5213
                     /vntifkey="4"
                     /label=Puro
     misc_feature    4554..4616
                     /vntifkey="21"
                     /label=T2A\Peptide
     misc_feature    3792..4547
                     /vntifkey="21"
                     /label=copGFP
     misc_feature    3726..3791
                     /vntifkey="21"
                     /label=P2A\peptide
BASE COUNT     2179 a      2341 c      2520 g      2094 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 
     1921 tatcgatagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat 
     1981 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc 
     2041 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct 
     2101 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 
     2161 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 
     2221 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct 
     2281 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc 
     2341 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag 
     2401 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa 
     2461 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt 
     2521 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag 
     2581 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc 
     2641 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt 
     2701 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc 
     2761 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc 
     2821 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg 
     2881 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca 
     2941 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt 
     3001 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga 
     3061 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt 
     3121 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc 
     3181 aggtgtcgtg agcaattgga tccaccatgg tcttcacact cgaagatttc gttggggact 
     3241 ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt gtgtccagtt 
     3301 tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg agcggtgaaa 
     3361 atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc ggcgaccaaa 
     3421 tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat cactttaagg 
     3481 tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg atcgactatt 
     3541 tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact gtaacaggga 
     3601 ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac ggctccctgc 
     3661 tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc attctggcga 
     3721 agcttggaag cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga 
     3781 accctggacc tatggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca 
     3841 tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca 
     3901 agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct 
     3961 acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct 
     4021 acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga 
     4081 agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg 
     4141 tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg 
     4201 acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc 
     4261 tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg 
     4321 tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc 
     4381 ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg 
     4441 agtaccagca cgccttcaag acccccattg ccttcgccag atcccgcgct cagtcgtcca 
     4501 attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgtc gacggaagcg 
     4561 gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggacctatga 
     4621 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca 
     4681 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc 
     4741 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 
     4801 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 
     4861 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 
     4921 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 
     4981 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg 
     5041 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 
     5101 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 
     5161 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagaat 
     5221 tcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 
     5281 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 
     5341 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 
     5401 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 
     5461 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 
     5521 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 
     5581 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt 
     5641 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 
     5701 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 
     5761 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 
     5821 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 
     5881 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt 
     5941 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 
     6001 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 
     6061 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 
     6121 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 
     6181 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
     6241 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
     6301 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt 
     6361 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 
     6421 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt 
     6481 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
     6541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 
     6601 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
     6661 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     6721 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     6781 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     6841 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     6901 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     6961 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     7021 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     7081 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     7141 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     7201 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     7261 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     7321 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 
     7381 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     7441 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     7501 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     7561 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 
     7621 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 
     7681 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
     7741 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
     7801 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
     7861 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
     7921 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
     7981 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
     8041 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
     8101 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 
     8161 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
     8221 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
     8281 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
     8341 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
     8401 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
     8461 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
     8521 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 
     8581 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 
     8641 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 
     8701 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc 
     8761 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 
     8821 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 
     8881 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 
     8941 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 
     9001 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 
     9061 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 
     9121 ccagtgccaa gctg 
//
