LOCUS       pCDH-CB-Nluc-P2A-copGFP-T2A-Puro        8668 bp    DNA     circular      8-DEC-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|752776742|
COMMENT     VNTDBDATE|752776831|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCDH-CB-Nluc-P2A-copGFP-T2A-Puro|
COMMENT     VNTAUTHORNAME|Demo User|
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COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2081696188 
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FEATURES             Location/Qualifiers
     misc_feature    4763..5353
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    5730..5861
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1798..1916
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    5425..5658
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(7204..8064)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     misc_feature    1309..1797
                     /vntifkey="21"
                     /label=env
     misc_feature    6386..7059
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     promoter        2226..2498
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=#800000
                     /vntifkey="29"
                     /label=chicken\beta\actin\promoter
     enhancer        1941..2225
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#80ffff
                     /vntifkey="9"
                     /label=CMV\promoter
     misc_feature    2737..3259
                     /vntifkey="21"
                     /label=Nluc
     misc_feature    2741..3253
                     /vntifkey="21"
                     /label=Nluc_ORF
     CDS             4151..4747
                     /vntifkey="4"
                     /label=Puro
     misc_feature    4088..4150
                     /vntifkey="21"
                     /label=T2A\Peptide
     misc_feature    3326..4081
                     /vntifkey="21"
                     /label=copGFP
     misc_feature    3260..3325
                     /vntifkey="21"
                     /label=P2A\peptide
BASE COUNT     2125 a      2234 c      2342 g      1967 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 
     1921 ttaattaagg tacctctggt cgttacataa cttacggtaa atggcccgcc tggctgaccg 
     1981 cccaacgacc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
     2041 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 
     2101 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
     2161 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctact 
     2221 cgaggccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta 
     2281 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggggggc 
     2341 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg 
     2401 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc 
     2461 ggcggcccta taaaaagcga agcgcgcggc gggcgggagc gggatcagcc accgcggtgg 
     2521 cggccctaga gtcgatcgag gaactgaaaa accagaaagt taactggtaa gtttagtctt 
     2581 tttgtctttt atttcaggtc cccgatccgg tggtggtgca aatcaaagaa ctgctcctca 
     2641 gtggatgttg cctttacttc taggcctgta cggaagtgtt acttctgctc taaaagctgc 
     2701 ggaattgtac ccgcggccga tccaccggtc gggatccacc atggtcttca cactcgaaga 
     2761 tttcgttggg gactggcgac agacagccgg ctacaacctg gaccaagtcc ttgaacaggg 
     2821 aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta actccgatcc aaaggattgt 
     2881 cctgagcggt gaaaatgggc tgaagatcga catccatgtc atcatcccgt atgaaggtct 
     2941 gagcggcgac caaatgggcc agatcgaaaa aatttttaag gtggtgtacc ctgtggatga 
     3001 tcatcacttt aaggtgatcc tgcactatgg cacactggta atcgacgggg ttacgccgaa 
     3061 catgatcgac tatttcggac ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat 
     3121 cactgtaaca gggaccctgt ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc 
     3181 cgacggctcc ctgctgttcc gagtaaccat caacggagtg accggctggc ggctgtgcga 
     3241 acgcattctg gcgaagcttg gaagcggagc tactaacttc agcctgctga agcaggctgg 
     3301 agacgtggag gagaaccctg gacctatgga gagcgacgag agcggcctgc ccgccatgga 
     3361 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg 
     3421 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct 
     3481 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac 
     3541 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa 
     3601 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta 
     3661 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag 
     3721 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat 
     3781 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta 
     3841 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct 
     3901 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga 
     3961 gctgggcatc gtggagtacc agcacgcctt caagaccccc attgccttcg ccagatcccg 
     4021 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg 
     4081 cgtcgacgga agcggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa 
     4141 tcctggacct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 
     4201 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 
     4261 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 
     4321 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 
     4381 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 
     4441 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 
     4501 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 
     4561 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 
     4621 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 
     4681 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 
     4741 cggtgcctga gaattcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac 
     4801 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 
     4861 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 
     4921 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 
     4981 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 
     5041 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 
     5101 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc 
     5161 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 
     5221 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 
     5281 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 
     5341 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 
     5401 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc 
     5461 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 
     5521 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 
     5581 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 
     5641 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 
     5701 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 
     5761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 
     5821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 
     5881 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 
     5941 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 
     6001 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt 
     6061 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 
     6121 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     6181 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 
     6241 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     6301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     6361 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     6421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     6481 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     6541 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     6601 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     6661 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     6721 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     6781 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     6841 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 
     6901 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     6961 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     7021 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     7081 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     7141 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     7201 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     7261 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     7321 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     7381 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     7441 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     7501 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     7561 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     7621 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     7681 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     7741 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     7801 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     7861 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     7921 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     7981 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     8041 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     8101 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     8161 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 
     8221 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 
     8281 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 
     8341 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 
     8401 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 
     8461 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 
     8521 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 
     8581 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 
     8641 ttgtaaaacg acggccagtg ccaagctg 
//
