LOCUS       pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro        8387 bp    DNA     circular      8-DEC-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|752777024|
COMMENT     VNTDBDATE|752777024|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     misc_feature    4482..5072
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7..234
                     /vntifkey="21"
                     /label=RSV
     misc_feature    5449..5580
                     /vntifkey="21"
                     /label=SV40\poly(A)
     misc_feature    1804..1921
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    5144..5377
                     /vntifkey="21"
                     /label=LTR
     misc_feature    complement(6923..7783)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    567..919
                     /vntifkey="21"
                     /label=Gag
     misc_feature    1076..1308
                     /vntifkey="21"
                     /label=RPE
     misc_feature    1309..1803
                     /vntifkey="21"
                     /label=env
     misc_feature    6105..6778
                     /vntifkey="21"
                     /label=pUC_ORI
     misc_feature    235..414
                     /vntifkey="21"
                     /label=LTR
     misc_feature    1929..2450
                     /vntifkey="21"
                     /label=PGK\Promoter
     misc_feature    2456..2978
                     /vntifkey="21"
                     /label=Nluc
     misc_feature    2460..2972
                     /vntifkey="21"
                     /label=Nluc_ORF
     CDS             3870..4466
                     /vntifkey="4"
                     /label=Puro
     misc_feature    3807..3869
                     /vntifkey="21"
                     /label=T2A\Peptide
     misc_feature    3045..3800
                     /vntifkey="21"
                     /label=copGFP
     misc_feature    2979..3044
                     /vntifkey="21"
                     /label=P2A\peptide
BASE COUNT     2044 a      2191 c      2265 g      1887 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 
     1921 tatcgataga tatcgaatta attctaccgg gtaggggagg cgcttttccc aaggcagtct 
     1981 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct 
     2041 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc 
     2101 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg 
     2161 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc 
     2221 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc 
     2281 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc 
     2341 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag 
     2401 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ggatccacca 
     2461 tggtcttcac actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg 
     2521 accaagtcct tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa 
     2581 ctccgatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca 
     2641 tcatcccgta tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg 
     2701 tggtgtaccc tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa 
     2761 tcgacggggt tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg 
     2821 tgttcgacgg caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg 
     2881 acgagcgcct gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga 
     2941 ccggctggcg gctgtgcgaa cgcattctgg cgaagcttgg aagcggagct actaacttca 
     3001 gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctatggag agcgacgaga 
     3061 gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt 
     3121 tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga 
     3181 agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg 
     3241 gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca 
     3301 acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg 
     3361 tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca 
     3421 ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg 
     3481 tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca 
     3541 gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg 
     3601 ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg 
     3661 agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca 
     3721 ttgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac 
     3781 ccggctccac cggatctcgc gtcgacggaa gcggagaggg cagaggaagt ctgctaacat 
     3841 gcggtgacgt cgaggagaat cctggaccta tgaccgagta caagcccacg gtgcgcctcg 
     3901 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc 
     3961 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag 
     4021 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 
     4081 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 
     4141 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 
     4201 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 
     4261 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 
     4321 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 
     4381 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct 
     4441 ggtgcatgac ccgcaagccc ggtgcctgag aattcgtcga caatcaacct ctggattaca 
     4501 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 
     4561 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 
     4621 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 
     4681 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 
     4741 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 
     4801 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 
     4861 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga 
     4921 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 
     4981 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 
     5041 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag 
     5101 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 
     5161 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 
     5221 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 
     5281 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 
     5341 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 
     5401 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 
     5461 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     5521 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
     5581 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 
     5641 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 
     5701 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat 
     5761 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 
     5821 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 
     5881 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 
     5941 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 
     6001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
     6061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
     6121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     6181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     6241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     6301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     6361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     6421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     6481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     6541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     6601 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     6661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     6721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     6781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     6841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     6901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     6961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     7021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     7081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     7141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     7201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     7261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     7321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     7381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     7441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     7501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     7561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     7621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     7681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     7741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     7801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     7861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 
     7921 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 
     7981 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 
     8041 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 
     8101 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag 
     8161 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 
     8221 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 
     8281 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 
     8341 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg 
//
