LOCUS pMB71 7993 bp ds-DNA circular 29-MAR-2015 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind 7848..7868 /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3695..3724 /label=MYC-tag 1 /ApEinfo_fwdcolor=#8080ff /ApEinfo_revcolor=#8080ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 6798..7480 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter join(16..153,155..414,416..3654) /label=elt-2 promoter /ApEinfo_fwdcolor=#cfa76e /ApEinfo_revcolor=#cfa76e /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3731..3760 /label=MYC-tag 2 /ApEinfo_fwdcolor=#8080ff /ApEinfo_revcolor=#8080ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 7820..7842 /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(3761..3886,3938..4184,4236..4426,4478..4687, 4739..4930) /label=BirA /ApEinfo_fwdcolor=#008080 /ApEinfo_revcolor=#008080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 6041..6700 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4986..5093 /label=let-858 UTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 AAGCTTGGGC TGCAGTAATT TCGAAATGTA TGAACTCCAA TTCTTGATAA CCAATTCCTG 61 ACTCTGTAAC TATTATTATT ATAATCCTAT TACCCACCTT TTTTCCCATT CTGCTCTTAG 121 TTCTCCCCCA ACCCGTACAT TTTCTCAACT AATgAAGACA ATGAGAAAGT GAGAGAGAAT 181 AAGACGAACT GAAAAGAATG TGTAAACAGT TTATTGCCTC TCGATTGTAC CATCTAGTGT 241 CTTGTCGTGT GCATAACTAC TGATAACAGA TCGAATGTGA AAGACCCAAT AAATGCTTGG 301 AAAGTATCAA ATATGTTTAG AAATAGTATT TAGAGACTTA TGTGGGACGA AAAAACAGGA 361 GCATTAAGAA TACAGTTGCA AACATTCATC AAGTTATAAT CTAATAATAT AAATcCAAAA 421 ATGTTAATGA TAAAAAATGA TGATCAAAGT AGGAAATTAG TGGCGAGAAA ACAATAAATC 481 ATCAGAAAAT GACTGATCTT GAGTAGAGTG CTTTTTTTGT AGATTTCTGA TTCTAAAACT 541 GAAAAAAAAA CTAAAAATGC TCGATGAGTC ATACTAATTG AAAAATAATC AGATTGAAAA 601 CGTTAAAAAT GTACTTGCTA ACATACTTCC ATGGTTATTT TTGAACACAA ATAAAGTTAT 661 ATACATCTTT ACCGGCACCA GAAGAAATCA CCGCTAACCA ATGTTCAGTT TTTACCTGAA 721 TATCCCTGAA AGTACAAAAA AATCAATGTT CAATCAAGTT TTTGCATGCC TTCGTGTGAT 781 GGCTCGCACC AAGTATTCTG ACAGTTATAA AATGTTTCCT GTGATCATGA ATTTGAAAAA 841 AAAAGAACTG ATGGGAAAAT GTGATATTCA TAAATGAAAT GTTTAATAAA CAAAAAAGAA 901 CCGTCATCAC AAATCTACTC TGAAACGTTC ACAGTTCCTA ATTGACCAAT TCAGAATTTT 961 TTGTACTGAC TTAATAAATA TAAATTTACA GTTTTAAGAA ACATAAATTG AATTATATAA 1021 ATAAAAAAAA AAAAACTAAA ATGAAGACGT GATGGAATGT TTCACGTATT AAAAAAGCTC 1081 TCGGACCGTT TGACATCCAG AAATTATGTG AAAGTGATTT TCTAACCCTT TGACGTCAAA 1141 AAAGTTTTTC TTTTTTGAAA GATACGCTGT TTAGAAATAC TTATACAGCT GATTTTAGAA 1201 TCTCCAAAAA ACCTTCAATT ACTTAACAAA CTTTCTAAAT AAAGAATTTT GATTTTGATT 1261 TTCTTTGAAC TTCAAACCCC CCGTTAAAAA ACCAAATTAA AAAAAAGATA CCAGATTAAT 1321 TTTAAAGTGA AAAATATAAA TTTATTCCCG ACTGTTAAAA ACGGTTTATA AACGGAGTCA 1381 CAACACTCAT ATACAACTAT CGACACCAAA TAAATAACTT CGAAAGCTAA TTGTGTCATT 1441 TCGGATATAG AGAGTAGTGT GAGCAGATGT GTTTTTGCAG AACTAAATTG ATGACACTAT 1501 TATGGAATAT AATGACCAAA TGTTAAATGT GTTAAGGTTT GATATCAAAA CCTGTATTTT 1561 CTTTTTATAC AGAATTGATA ATGTTATCTT CAATTGATTT CTACTTCTGA GCTACGGCGA 1621 TACGAGGACG CATTCTCAAC GATAATGTTG CCATTTTGTC CTGATAATTT TTTTACTGAT 1681 TGTTTCAGAA CACCCATAGT TTTTCTCTAT TAAACGTTCA TCCTTGACTT CCCCGAGTTT 1741 GCTGGCTGAA TAGGAAATTT GAAGACAAAA AGGAAAGAAT CGGCTCAAAC GTCATGCAAC 1801 TGATAAGGCG ACCGTACTTC ATTTCAAAAG AAGCTCACTT ACTGAGCGCA AACATTGAGA 1861 AATGAGAGCA AAAGAAGTGG TTTTACAACA TGCTAATGTT TTTAGACCTT GTACCCAATA 1921 ATATTACTGT AGTATACAGT TCGGAGAGCA TATGGTTGAA ATCTTGAAAT ACCAATTTAT 1981 CACTAGTTTG ATTGTGTTAT CGATGTATAA AGATATATTT TATCATTTTT GATTATTATC 2041 TGATATTGTG GGGTGTGAAG TAATATTATG TGCGTGTGTG GCTGATTATC GAAAAAAACT 2101 GAAAATTATC AATTTTTCTA CAGGTTATCT TTTTTTGTTT TATTTTTCAT TATTGTATTC 2161 TTCATACTCC TTATCCTGCC CGAATCACAA AAGTTCCAGA CAACTTGAAG GTCGCATCAC 2221 GTTTTGTTTA TAAGAATGTT GAACTGGTCA GCTTTGGAGA CAGAACTGTC CTCCAAATGT 2281 TGAATATGTT TCCGTAAAAC ACACTGTGTA ACAAAAAGCG TACCCTTTGA TCCGGTATTC 2341 TTGAAAAAAA ACATAGAAGG GCGTACTTTT CATATCTCAC AAATATGCGT TTTTTATTTT 2401 ATAATAATAT CATTTATTGA TAATTATATG TATTTGTATG TTTTCTTGTC TTTAAAATTA 2461 TGGTGTGACA TCACAGTTTA AAAATTATAT GAAAACTAAT GAGTGAAAAG TTAAAAATGT 2521 ATAGTTTGCC GTATTTTCTC CATTAGTATT GTATTCAAAA CTTATTTCCA ATTGGTGAGC 2581 ACTATAAACT TTGTAGGGTA ATTGAGGTTC AATACTAATT TTTGAATATC TAGCTTTTGA 2641 CAAATTATTC AAGTTTTGAC AATGATGCCT TATCAAATTA AAAAAAATTA CCATAAATTC 2701 TTGAAAGCTT TTGATTTTGT TTCACTCTGT GAAGGATAGT AATTTATCAA CATTTTAACT 2761 AGATACATCT GTTCTGGCAA TAAATTAAAG CAAGACTATT ACTATGTGCA GTATTAATAG 2821 CTGCATTGCA ACTTGTTTGA AAGTTTCAGT GAAAACGCAA AAACTCAATT TAATTTTCAA 2881 GTTTGTTTGC AAAATTGGAT ATATGTATCC ATCAAAAGTC TGATCGCCTG TTTTATCTAC 2941 TTATTGTCTC CTCAGTTGAT TTTTTTGTAG TTATTATAAA CGCTACTTTG AAACCAATAT 3001 TTTCTTCCTC ATTCCACTTT TTTCAATTAC CAACTACCGT ACATGCAATG ATGGGCGGAC 3061 CCGAGTCCCA TGTTTGGCTG GAAGTGGGTG GTTGTCTGCG TATATGAGCG ACAGAGGTCG 3121 GGGCTGAAAC TGATAAGAAT AGTCGACACT AACGCCATAA TCGCTAGCCA GCCATCATGC 3181 ACACCGAGCT CGGTGTGCAC ACCATCTTTC TTTTCAAACC AATACGCTTT GTGCCTTCAT 3241 TGACAATTTT CTTTTTGATA AAATCAGCCT ATCTATACTT CCCAATCATT TTTAGTCTTA 3301 TCGTTGAACA GCTATCGAGG TGCCACTGTT TTCACTGATA TCTTCTAAGT TACTATGGCA 3361 TTAACATCTT TGTGTTCTTA TTTTATGGGT TATTTTAATT AATTTTTGCA GTTAATTTTT 3421 GGAATGAGCA AGAAAATGTT AATTGTAATA TCTTCGTCTG AAAATTGTCT TCAAATAGTT 3481 TTAATTTTAA AGGCAGTATT TAAGAAAATA CACTTCTCGA AGCATTTTTG AATTTTTGAA 3541 TTTTTAAACT GCTTGATGTT TTAGGTGCCA CTGTTTTCAT TGATAAGTTT TGATGTATAA 3601 ATGCTTGATT TTCTTGGCAT TCTAATAAAA TAGAAACTAG AAAATAGATT ATAGCCCGGG 3661 TGCCCTATAG TGAGTCGTAT TGGTACCaaa aATGGAGCAG AAGCTCATCT CTGAGGAAGA 3721 CCTCGGAGGA GAGCAGAAAC TCATCTCTGA AGAGGACCTC ATGAAGGATA ATACTGTTCC 3781 ATTGAAGTTG ATTGCTTTGC TTGCAAATGG AGAATTTCAT TCTGGAGAAC AACTTGGAGA 3841 GACGTTGGGA ATGTCGCGTG CTGCAATTAA CAAGCATATT CAAACAgtaa gtttaaacag 3901 ttcggtacta actaaccata catatttaaa ttttcagTTG AGAGACTGGG GAGTTGACGT 3961 TTTTACGGTT CCAGGAAAGG GATATTCGCT TCCAGAGCCA ATTCAGCTCT TGAATGCAAA 4021 GCAAATTTTG GGACAGCTTG ATGGTGGTAG TGTTGCAGTA CTTCCGGTTA TAGATAGTAC 4081 AAATCAGTAC TTGCTTGATC GAATAGGAGA ATTGAAATCA GGAGATGCTT GCATCGCTGA 4141 GTATCAGCAG GCTGGACGAG GACGAAGAGG ACGTAAGTGG TTCTgtaagt ttaaacatat 4201 atatactaac taaccctgat tatttaaatt ttcagCGCCT TTCGGAGCCA ATCTCTATTT 4261 GAGTATGTTC TGGCGTCTTG AACAAGGTCC GGCCGCTGCT ATCGGACTTT CATTGGTTAT 4321 TGGAATTGTG ATGGCAGAAG TTCTCCGAAA ACTTGGTGCA GATAAGGTGA GAGTCAAGTG 4381 GCCTAACGAT CTTTATCTTC AAGATAGAAA ATTGGCCGGA ATATTGgtaa gtttaaacat 4441 atatatacta actaaccctg attatttaaa ttttcagGTT GAGCTTACTG GAAAGACGGG 4501 AGATGCTGCT CAAATTGTGA TTGGAGCTGG TATCAATATG GCAATGAGAA GAGTAGAAGA 4561 GTCTGTTGTT AATCAAGGTT GGATCACACT TCAAGAGGCA GGAATCAACC TTGATAGAAA 4621 TACGTTGGCC GCCATGCTTA TCCGAGAATT GCGAGCTGCA CTCGAACTCT TCGAACAAGA 4681 AGGTCTTgta agtttaaaca tatatatact aactaaccct gattatttaa attttcagGC 4741 CCCTTACCTC AGTAGATGGG AGAAACTTGA CAATTTTATC AATCGACCAG TGAAGTTGAT 4801 AATTGGAGAT AAGGAAATTT TTGGTATCAG TCGAGGAATT GATAAGCAAG GAGCTCTTCT 4861 CCTTGAGCAA GATGGAATCA TTAAACCATG GATGGGTGGA GAGATTTCTT TGCGATCTGC 4921 TGAAAAGTAA GAATTCGCTA GCCGGCCATA CAAGTAATCC GGATGATCGA CGCCaACGTC 4981 GTTGAATTTT CAAATTTTAA ATACTGAATA TTTGTTTTTT TTCCTATTAT TTATTTATTC 5041 TCTTTGTGTT TTTTTTCTTG CTTTCTAAAA AATTAATTCA ATCCAAATCT AAacattttt 5101 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag 5161 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa 5221 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc 5281 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact 5341 agtaaaaaat aagcaaagaa attaggcgaa cgatgAGAAT TGTCCTCGCT TGGGCCCTTT 5401 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG 5461 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 5521 GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA 5581 GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG 5641 CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT 5701 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT 5761 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA 5821 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT 5881 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA 5941 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA 6001 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT 6061 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT 6121 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA 6181 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC 6241 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT 6301 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA 6361 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC 6421 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA 6481 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC 6541 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC 6601 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG 6661 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA 6721 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA 6781 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC 6841 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 6901 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 6961 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 7021 CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 7081 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 7141 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 7201 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 7261 TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 7321 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 7381 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 7441 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 7501 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG 7561 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA 7621 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG 7681 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG 7741 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT 7801 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA 7861 TGACCATGAT TACGCCAAGC Tgtaagttta aacatgatct tactaactaa ctattctcat 7921 ttaaattttc agAGCTTAAA AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC 7981 TTGGAAATGA AAT //