LOCUS       pMB41___N_term_1        5052 bp ds-DNA     linear       21-FEB-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     378..395
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(2827..2847)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    416..460
                     /label=Avi tag
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(3215..3897)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    931..964
                     /label=FRT site 1
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(239..307)
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2222..2255
                     /label=FRT site 2
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    complement(2853..2875)
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    965..2221
                     /label=galK
                     /ApEinfo_fwdcolor=#8080c0
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3995..4654)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    476..517
                     /label=TEV site
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(533..703,755..908,2285..2448,2500..2724)
                     /label=GFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2725..2763
                     /label=Flexible linker
                     /ApEinfo_fwdcolor=#8080ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCttA GATCTGGACT
      421 TAATGATATT TTCGAAGCTC AgAAgATTGA ATGGCATGAA tctggaggag gatctGAgAA
      481 cCTTTAcTTC CAAGGaGAgA AcCTTTAcTT CCAAGGatct ggaggaggat ctATGAGTAA
      541 AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA
      601 TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC
      661 CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata
      721 tatactaact aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCTGTTA
      781 TGGTGTTCAA TGCTTCTCGA GATACCCAGA TCATATGAAA CGGCATGACT TTTTCAAGAG
      841 TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA
      901 CAAGACACgt aagtttaaac agttcggtac gaagttccta tactttctag agaataggaa
      961 cttccctgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     1021 gaggaactaa acccaggagg cagatcatga gtctgaaaga aaaaacacaa tctctgtttg
     1081 ccaacgcatt tggctaccct gccactcaca ccattcaggc gcctggccgc gtgaatttga
     1141 ttggtgaaca caccgactac aacgacggtt tcgttctgcc ctgcgcgatt gattatcaaa
     1201 ccgtgatcag ttgtgcacca cgcgatgacc gtaaagttcg cgtgatggca gccgattatg
     1261 aaaatcagct cgacgagttt tccctcgatg cgcccattgt cgcacatgaa aactatcaat
     1321 gggctaacta cgttcgtggc gtggtgaaac atctgcaact gcgtaacaac agcttcggcg
     1381 gcgtggacat ggtgatcagc ggcaatgtgc cgcagggtgc cgggttaagt tcttccgctt
     1441 cactggaagt cgcggtcgga accgtattgc agcagcttta tcatctgccg ctggacggcg
     1501 cacaaatcgc gcttaacggt caggaagcag aaaaccagtt tgtaggctgt aactgcggga
     1561 tcatggatca gctaatttcc gcgctcggca agaaagatca tgccttgctg atcgattgcc
     1621 gctcactggg gaccaaagca gtttccatgc ccaaaggtgt ggctgtcgtc atcatcaaca
     1681 gtaacttcaa acgtaccctg gttggcagcg aatacaacac ccgtcgtgaa cagtgcgaaa
     1741 ccggtgcgcg tttcttccag cagccagccc tgcgtgatgt caccattgaa gagttcaacg
     1801 ctgttgcgca tgaactggac ccgatcgtgg caaaacgcgt gcgtcatata ctgactgaaa
     1861 acgcccgcac cgttgaagct gccagcgcgc tggagcaagg cgacctgaaa cgtatgggcg
     1921 agttgatggc ggagtctcat gcctctatgc gcgatgattt cgaaatcacc gtgccgcaaa
     1981 ttgacactct ggtagaaatc gtcaaagctg tgattggcga caaaggtggc gtacgcatga
     2041 ccggcggcgg atttggcggc tgtatcgtcg cgctgatccc ggaagagctg gtgcctgccg
     2101 tacagcaagc tgtcgctgaa caatatgaag caaaaacagg tattaaagag actttttacg
     2161 tttgtaaacc atcacaagga gcaggacagt gctgaggatc cactagttct agagcggccg
     2221 cgaagttcct atactttcta gagaatagga acttctaact aaccatacat atttaaattt
     2281 tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT
     2341 ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA
     2401 CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac
     2461 atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC
     2521 ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT
     2581 GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT
     2641 CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA
     2701 CATGGCATGG ATGAACTATA CAAAggagga ggatctggag gaggaggatc tggaggagga
     2761 ggtTCCGGAT TCCATCGGAT CCCGGGCCCG TCGACTGCAG AGGCCTGCAT GCAAGCTTGG
     2821 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
     2881 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
     2941 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
     3001 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT
     3061 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
     3121 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
     3181 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
     3241 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
     3301 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
     3361 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
     3421 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
     3481 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
     3541 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
     3601 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
     3661 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
     3721 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
     3781 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
     3841 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
     3901 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
     3961 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
     4021 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
     4081 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC
     4141 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
     4201 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
     4261 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
     4321 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
     4381 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
     4441 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
     4501 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
     4561 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
     4621 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
     4681 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
     4741 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
     4801 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
     4861 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
     4921 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
     4981 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
     5041 GGCCCTTTCG TC    
//
