LOCUS                  pMB72        5052 bp ds-DNA   circular     17-AUG-2011
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     378..395
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    2681..2701
                     /label=TEV site
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
     misc_feature    696..849
                     /label=GFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     primer_bind     complement(2827..2847)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    872..905
                     /label=FRT site
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
     misc_feature    2741..2785
                     /label=Avi tag
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=#0000ff
     misc_feature    2163..2196
                     /label=FRT site(1)
                     /ApEinfo_label=FRT site
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
     rep_origin      complement(3215..3897)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     misc_feature    906..2162
                     /label=galK
                     /ApEinfo_fwdcolor=#800040
                     /ApEinfo_revcolor=green
     misc_feature    join(2705..2706,2708..2709,2711..2715,2717..2725)
                     /label=TEV site(1)
                     /ApEinfo_label=TEV site
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
     misc_feature    2226..2389
                     /label=GFP(1)
                     /ApEinfo_label=GFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     CDS             complement(239..307)
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
     misc_feature    2441..2510
                     /label=GFP(2)
                     /ApEinfo_label=GFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     misc_binding    complement(2853..2875)
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
     CDS             complement(3995..4654)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     misc_feature    2681..2725
                     /label=2 TEV sites
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
     misc_feature    2511..2665
                     /label=GFP(3)
                     /ApEinfo_label=GFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     misc_feature    474..644
                     /label=GFP(4)
                     /ApEinfo_label=GFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCCGGGGAT
      421 CCTCTAGATC CGGAggagga ggatctggag gaggaggatc tggaggagga ggaATGAGTA
      481 AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT GGTGATGTTA
      541 ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC GGAAAACTTA
      601 CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGgtaagt ttaaacatat
      661 atatactaac taaccctgat tatttaaatt ttcagccaac acttgtcact actttctgtt
      721 atggtgttca atgcttctcg agatacccag atcatatgaa acggcatgac tttttcaaga
      781 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      841 acaagacacg taagtttaaa cagttcggta cgaagttcct atactttcta gagaatagga
      901 acttccctgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
      961 tgaggaacta aacccaggag gcagatcatg agtctgaaag aaaaaacaca atctctgttt
     1021 gccaacgcat ttggctaccc tgccactcac accattcagg cgcctggccg cgtgaatttg
     1081 attggtgaac acaccgacta caacgacggt ttcgttctgc cctgcgcgat tgattatcaa
     1141 accgtgatca gttgtgcacc acgcgatgac cgtaaagttc gcgtgatggc agccgattat
     1201 gaaaatcagc tcgacgagtt ttccctcgat gcgcccattg tcgcacatga aaactatcaa
     1261 tgggctaact acgttcgtgg cgtggtgaaa catctgcaac tgcgtaacaa cagcttcggc
     1321 ggcgtggaca tggtgatcag cggcaatgtg ccgcagggtg ccgggttaag ttcttccgct
     1381 tcactggaag tcgcggtcgg aaccgtattg cagcagcttt atcatctgcc gctggacggc
     1441 gcacaaatcg cgcttaacgg tcaggaagca gaaaaccagt ttgtaggctg taactgcggg
     1501 atcatggatc agctaatttc cgcgctcggc aagaaagatc atgccttgct gatcgattgc
     1561 cgctcactgg ggaccaaagc agtttccatg cccaaaggtg tggctgtcgt catcatcaac
     1621 agtaacttca aacgtaccct ggttggcagc gaatacaaca cccgtcgtga acagtgcgaa
     1681 accggtgcgc gtttcttcca gcagccagcc ctgcgtgatg tcaccattga agagttcaac
     1741 gctgttgcgc atgaactgga cccgatcgtg gcaaaacgcg tgcgtcatat actgactgaa
     1801 aacgcccgca ccgttgaagc tgccagcgcg ctggagcaag gcgacctgaa acgtatgggc
     1861 gagttgatgg cggagtctca tgcctctatg cgcgatgatt tcgaaatcac cgtgccgcaa
     1921 attgacactc tggtagaaat cgtcaaagct gtgattggcg acaaaggtgg cgtacgcatg
     1981 accggcggcg gatttggcgg ctgtatcgtc gcgctgatcc cggaagagct ggtgcctgcc
     2041 gtacagcaag ctgtcgctga acaatatgaa gcaaaaacag gtattaaaga gactttttac
     2101 gtttgtaaac catcacaagg agcaggacag tgctgaggat ccactagttc tagagcggcc
     2161 gcgaagttcc tatactttct agagaatagg aacttctaac taaccataca tatttaaatt
     2221 ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
     2281 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
     2341 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa
     2401 catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa
     2461 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc CAATTGGCGA
     2521 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA
     2581 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC
     2641 ACATGGCATG GATGAACTAT ACAAAtctgg aggaggatct GAAAATCTTT ATTTCCAAGG
     2701 AtccGAgAAc CTTTAcTTCC AAGGAtctgg aggaggatct GGACTTAATG ATATTTTCGA
     2761 AGCTCAgAAg ATTGAATGGC ATGAAtaaAG ATCTGTCGAC CTGCAGGCAT GCAAGCTTGG
     2821 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
     2881 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
     2941 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
     3001 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT
     3061 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
     3121 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
     3181 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
     3241 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
     3301 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
     3361 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
     3421 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
     3481 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
     3541 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
     3601 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
     3661 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
     3721 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
     3781 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
     3841 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
     3901 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
     3961 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
     4021 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
     4081 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC
     4141 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
     4201 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
     4261 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
     4321 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
     4381 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
     4441 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
     4501 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
     4561 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
     4621 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
     4681 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
     4741 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
     4801 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
     4861 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
     4921 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
     4981 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
     5041 GGCCCTTTCG TC    
//
