LOCUS       645_pKAN_1xmCher        5951 bp    DNA     circular SYN 11-JAN-2016
DEFINITION  Ligation of BamHI-1xmCherry-SP6-BglII into Cut BamHI-pKAN 12XPP7 V4
ACCESSION   645_pKAN_1xmCher
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  Cut BamHI-pKAN 12XPP7 V4
               Fragment ends: BamHI
               Fragment size: 5192
            Insert molecule:  BamHI-1xmCherry-BglII
               Fragment ends: BamHI and BglII
               Fragment size: 759
FEATURES             Location/Qualifiers
     CDS             60..770
                     /gene="mCherry"
                     /SECDrawAs="Gene"
     misc_feature    804..1283
                     /gene="12xPP7 V4"
                     /SECDrawAs="Region"
     misc_signal     817..841
                     /gene="(null)"
                     /SECDrawAs="Label"
     misc_signal     862..886
                     /gene="(null)"
                     /SECDrawAs="Label"
     misc_signal     895..919
                     /gene="(null)"
                     /SECDrawAs="Label"
     misc_signal     940..964
                     /gene="(null)"
                     /SECDrawAs="Label"
     misc_signal     973..997
                     /gene="(null)"
                     /SECDrawAs="Label"
     misc_signal     1018..1042
                     /gene="(null)"
                     /SECDrawAs="Label"
     misc_signal     1051..1075
                     /gene="(null)"
                     /SECDrawAs="Label"
     misc_signal     1252..1276
                     /gene="(null)"
                     /SECDrawAs="Label"
     misc_feature    1299..1332
                     /gene="Lox-P"
                     /SECDrawAs="Region"
     CDS             1338..1570
                     /gene="ADH1"
                     /SECDrawAs="Gene"
     CDS             1959..2768
                     /gene="KanR"
                     /product="KanR"
                     /SECDrawAs="Gene"
     misc_feature    3034..3067
                     /gene="Lox-P"
                     /SECDrawAs="Region"
     CDS             complement (4318..5178)
                     /gene="AmpR"
                     /product="Ampi"
                     /SECDrawAs="Gene"
ORIGIN      
        1 cgaattggag ctccaccgcg gtggcggccg ctctagaact agtggatccg gtcgccacca
       61 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
      121 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      181 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
      241 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
      301 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
      361 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
      421 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
      481 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
      541 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
      601 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
      661 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
      721 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa catacgattt
      781 aggtgacact atagttaatt aaagatccat gcagaaccag cagagcatat gggctcgctg
      841 gctgcacgtc gactctagaa accagcagag catatgggct cgctggatgc agaaccagca
      901 gagcatatgg gctcgctggc tgcacgtcga ctctagaaac cagcagagca tatgggctcg
      961 ctggatgcag aaccagcaga gcatatgggc tcgctggctg cacgtcgact ctagaaacca
     1021 gcagagcata tgggctcgct ggatgcagaa ccagcagagc atatgggctc gctggctgca
     1081 cgtcgactct agaaaccagc agagcatatg ggctcgctgg atgcagaacc agcagagcat
     1141 atgggctcgc tggctgcacg tcgactctag aaaccagcag agcatatggg ctcgctggat
     1201 gcagaaccag cagagcatat gggctcgctg gctgcacgtc gactctagaa accagcagag
     1261 catatgggct cgctggatgc atagatctaa cccttaatat aacttcgtat aatgtatgct
     1321 atacgaagtt attaggtgat ctgccacttc taaataagcg aatttcttat gatttatgat
     1381 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     1441 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     1501 tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga
     1561 taacagggtg gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
     1621 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
     1681 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
     1741 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
     1801 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
     1861 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
     1921 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg
     1981 tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     2041 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
     2101 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     2161 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     2221 ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat
     2281 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     2341 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     2401 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     2461 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac
     2521 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     2581 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     2641 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     2701 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     2761 ttttctaatc agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata
     2821 gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt
     2881 tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt
     2941 caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc
     3001 agtgtcgaaa acgagctctc gagaaccctt aatataactt cgtataatgt atgctatacg
     3061 aagttattag gtgatatcag atccactagt ggcctatgcg gtacccagct tttgttccct
     3121 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3181 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3241 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3301 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3361 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3421 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3481 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3961 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4141 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4201 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4261 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4321 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4381 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4441 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4501 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4561 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4621 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4681 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4741 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4801 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4861 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4921 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4981 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5041 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5101 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5161 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5221 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5281 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
     5341 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5401 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5461 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5521 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5581 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5641 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5701 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     5761 aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     5821 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     5881 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     5941 tcactatagg g
//
