LOCUS pCen1-CRISPR 11917 bp DNA circular SYN 11-JAN-2016 DEFINITION . ACCESSION pCen1MCS SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5892) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-11058478| COMMENT VNTDBDATE|-11058478| COMMENT LSOWNER| COMMENT VNTNAME|pCen1-CRISPR| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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/ApEinfo_fwdcolor=#ffff80 /ApEinfo_revcolor=#ff0000 /vntifkey="21" /label=LEU2 misc_feature 9959..11176 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /vntifkey="21" /label=Leu2ORF\ CDS complement(856..4992) /vntifkey="4" /label=hCas9 promoter complement(4993..5523) /vntifkey="29" /label=TEFin terminator complement(655..855) /vntifkey="43" /label=cyc1t terminator complement(5530..6030) /vntifkey="43" /label=mig1t snoRNA complement(6031..6098) /vntifkey="105" /label=HDV snRNA complement(6099..6178) /vntifkey="57" /label=sgRNA snoRNA 6199..6235 /vntifkey="105" /label=HH insertion_seq 6179..6198 /vntifkey="14" /label=trp1.gRNA promoter complement(6242..6772) /vntifkey="29" /label=TEFin BASE COUNT 2856 a 2890 c 2932 g 3239 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccgctttt 421 cgtagataat ggaatacaaa tggatatcca gagtatacac atggatagta tacactgaca 481 cgacaattct gtatctcttt atgttaacta ctgtgaggcg ttaaatagag cttgatatat 541 aaaatgttac atttcacagt ctgaactttt gcagattacc taatttggta agatattaat 601 tatgaactga aagttgatgg catccctaaa tttgatgaaa gggggatcac gcgtctgtac 661 agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat aactataaaa 721 aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat 781 gtggggggag ggcgtgaatg taagcgtgac ataactaatt acatgactcg aggtccaatg 841 actcgaggtc caaaatcaca ccttcctctt cttcttgggg tcagccctgc tgtctccacc 901 gagctgagag aggtcgattc ttgtttcata gagccccgta attgactgat gaatcagtgt 961 ggcgtccagg acctcctttg tagaggtgta ccgctttctg tctatggtgg tgtcgaagta 1021 cttgaaggct gcaggcgcgc ccaagttggt cagagtaaac aagtggataa tgttttctgc 1081 ctgctccctg atgggcttat ccctgtgctt attgtaagca gaaagcacct tatcgaggtt 1141 agcgtcggcg aggatcactc ttttggagaa ttcgcttatt tgctcgatga tctcatcaag 1201 gtagtgtttg tgttgttcca cgaacagctg cttctgctca ttatcttcgg gagacccttt 1261 gagcttttca tagtggctgg ccagatacaa gaaattaacg tatttagagg gcagtgccag 1321 ctcgttacct ttctgcagct cgcccgcact agcgagcatt cgtttccggc cgttttcaag 1381 ctcaaagaga gagtacttgg gaagcttaat gatgaggtct tttttgacct ctttatatcc 1441 tttcgcctcg agaaagtcga tggggttttt ttcgaagctt gatcgctcca tgattgtgat 1501 gcccagcagt tccttgacgc ttttgagttt tttagacttc cctttctcca ctttggccac 1561 aaccagtaca ctgtaagcga ctgtaggaga atcgaatccg ccgtatttct tggggtccca 1621 atcttttttg cgtgcgatca gcttgtcgct gttccttttc gggaggatac tttccttgga 1681 gaagcctccg gtctgtactt cggtcttttt aacgatgttc acctgcggca tggacaggac 1741 cttccggact gtcgcgaaat ccctaccctt gtcccacacg atttctcctg tttctccgtt 1801 tgtttcgata agtggtcgct tccgaatctc tccattggcc agtgtaatct cggtcttgaa 1861 aaaattcata atattgctgt aaaagaagta cttagcggtg gccttgccta tttcctgctc 1921 agactttgcg atcattttcc taacatcgta cactttatag tctccgtaaa caaattcaga 1981 ttcaagcttg ggatattttt tgataagtgc agtgcctacc actgcattca ggtaggcatc 2041 atgcgcatgg tggtaattgt tgatctctct caccttataa aactgaaagt cctttctgaa 2101 atctgagacc agcttagact tcagagtaat aactttcacc tctcgaatca gtttgtcatt 2161 ttcatcgtac ttggtgttca tgcgtgaatc gagaatttgg gccacgtgct tggtgatctg 2221 gcgtgtctca acaagctgcc ttttgatgaa gccggcttta tccaactcag acaggccacc 2281 tcgttcagcc ttagtcagat tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag 2341 ctgccgccaa taatttttca ttttcttgac aacttcttct gaggggacgt tatcactctt 2401 ccctctattt ttatcggatc ttgtcaacac tttattatca atagaatcat ctttgagaaa 2461 agactggggc acgatatgat ccacgtcgta gtcggagagc cgattgatgt ccagttcctg 2521 atccacgtac atgtccctgc cgttctgcag gtagtacagg tagagcttct cattctgaag 2581 ctgggtgttt tcaactgggt gttccttaag gatttgggac cccagttctt ttataccctc 2641 ttcaatcctc ttcatccttt ccctactgtt cttctgtccc ttctgggtag tttggttctc 2701 tcgggccatc tcgataacga tattctcggg cttatgcctt cccattactt tgacgagttc 2761 atccacgacc ttaacggtct gcagtattcc ctttttgata gctgggctac ctgcaagatt 2821 agcgatgtgc tcgtgaagac tgtccccctg gccagaaact tgtgctttct ggatgtcctc 2881 cttaaaggtg agagagtcat catggatcaa ctgcatgaag ttccggttgg caaatccatc 2941 ggacttaaga aaatccagga ttgtctttcc actctgcttg tctcggatcc cattgatcag 3001 ttttcttgac agccgccccc atcctgtata tcggcgcctc ttgagctgtt tcatgacttt 3061 gtcgtcgaag agatgagcgt aagttttcaa gcgttcttca atcatctccc tatcttcaaa 3121 caacgtaagg gtgaggacaa tgtcctcaag aatgtcctcg ttctcctcat tgtccaggaa 3181 gtccttgtct ttaatgattt tcaggagatc gtgatacgtt cccagggatg cgttgaagcg 3241 atcctccact ccgctgattt caacagagtc gaaacattca atctttttga aatagtcttc 3301 tttgagctgt ttcacggtaa ctttccggtt cgtcttgaag aggaggtcca cgatagcttt 3361 cttctgctct ccagacagga atgctggctt tctcatccct tctgtgacgt atttgacctt 3421 ggtgagctcg ttataaactg tgaagtactc gtacagcaga gagtgtttag gaagcacctt 3481 ttcgttaggc agatttttat caaagttagt catcctttcg atgaaggact gggcagaggc 3541 ccccttatcc acgacttcct cgaagttcca gggagtgatg gtctcttctg atttgcgagt 3601 catccacgcg aatctggaat ttccccgggc gagggggcct acatagtagg gtatccgaaa 3661 tgtgaggatt ttctcaatct tttccctgtt atctttcaaa aaggggtaga aatcctcttg 3721 ccgcctgagg atagcgtgca gttcgcccag gtgaatctgg tgggggatgc ttccattgtc 3781 gaaagtgcgc tgtttgcgca acagatcttc tctgttaagc tttaccagca gctcctcggt 3841 gccgtccatt ttttccaaga tgggcttaat aaatttgtaa aattcctcct ggcttgctcc 3901 gccgtcaatg tatccggcgt agccattttt agactgatcg aagaaaattt ccttgtactt 3961 ctcaggcagt tgctgtctga caagggcctt cagcaaagtc aagtcttggt ggtgctcatc 4021 atagcgcttg atcatactag cgctcagcgg agctttggtg atctccgtgt tcactcgcag 4081 aatatcactc agcagaatgg cgtctgacag gttctttgcc gccaaaaaaa ggtctgcgta 4141 ctggtcgccg atctgggcca gcagattgtc gagatcatca tcgtaggtgt ctttgctcag 4201 ttgaagcttg gcatcttcgg ccaggtcgaa gttagattta aagttggggg tcagcccgag 4261 tgacagggcg ataagattac caaacaggcc gttcttcttc tccccaggga gctgtgcgat 4321 gaggttttcg agccgccggg atttggacag cctagcgctc aggattgctt tggcgtcaac 4381 tccggatgcg ttgatcgggt tctcttcgaa aagctgattg taagtctgaa ccagttggat 4441 aaagagtttg tcaacatcgc tgttgtctgg gttcaggtcc ccctcgatga ggaagtgtcc 4501 ccgaaatttg atcatatgcg ccagcgcgag atagatcaac cgcaagtcag ccttatcagt 4561 actgtctaca agcttcttcc tcagatgata tatggttggg tacttttcat ggtacgccac 4621 ctcgtccacg atattgccaa agattgggtg gcgctcgtgc tttttatcct cctccaccaa 4681 aaaggactcc tccagcctat ggaagaaaga gtcatccacc ttagccatct cattactaaa 4741 gatctcctgc aggtagcaga tccgattctt tctgcgggta tatctgcgcc gtgctgttct 4801 tttgagccgc gtggcttcgg ccgtctcccc ggagtcgaac aggagggcgc caatgaggtt 4861 cttctttatg ctgtggcgat cggtattgcc cagaactttg aattttttgc tcggcacctt 4921 gtactcgtcc gtaatgacgg cccagccgac gctgtttgtg ccgatatcga gcccaatgga 4981 gtacttcttg tcctgcggtt agtactgcaa aaagtgctgg tcggatgacg tggcgtcttg 5041 tgtcgatggg aatgggacca cacccggccg tgtgctcaaa gcccgtggca attgctgctg 5101 cctctgaaac tcaccatttt gaatgattct tatactcaga aggaaatgct taacgatttc 5161 gggtgtgagt tgacaaggag agagagaaaa gaagaggaaa ggtaattcgg ggacggtggt 5221 cttttatacc cttggctaaa gtcccaacca caaagcaaaa aaattttcag tagtctattt 5281 tgcgtccggc atgggttacc cggatggcca gacaaagaaa ctagtacaaa gtctgaacaa 5341 gcgtagattc cagactgcag taccctacgc ccttaacggc aagtgtggga accgggggag 5401 gtttgatatg tggggtgaag ggggctctcg ccggggttgg gcccgctact gggtcaattt 5461 ggggtcaatt ggggcaattg gggctgtttt ttgggacaca aatacgccgc caacccggtc 5521 tctgtcgaca aacccaaaag ggccgaaggc tgggccgagg ctctgagatg gatgagatag 5581 ctggggaaaa caggtgagta aatgattagt aatcagacac ccaggcagat ccgatacacc 5641 ggtatgattg catcacgtga cttcattgct cctccacaga gagattagtc atgctctttt 5701 tcagaatcgc aaacccttcc cccacttact taacccctca agctcaactg aaacagtcac 5761 tcactgtaga tgtgctccga aggcccagga acaatgtggg gtaggaaaat attatgtcta 5821 tacgagacga atacggtcac cgactctaat gtaagtgata ccatgtatag tagtgtcttc 5881 tggtacagaa ctccaagtaa tgtgtactta atcccctgta atacaatacc gtgctctcat 5941 tctcttacgg tatcattaca ttcatctata tacagccaat gggcaatgcg tgtgctgagc 6001 tcagcacgtt aaattatcga ccggccagtg gtcccattcg ccatgccgaa gcatgttgcc 6061 cagccggcgc cagcgaggag gctgggacca tgccggccaa aagcaccgac tcggtgccac 6121 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaaccg 6181 atggcgtcct gatccagtga cgagcttact cgtttcgtcc tcacggactc atcagactgg 6241 actgcggtta gtactgcaaa aagtgctggt cggatgacgt ggcgtcttgt gtcgatggga 6301 atgggaccac acccggccgt gtgctcaaag cccgtggcaa ttgctgctgc ctctgaaact 6361 caccattttg aatgattctt atactcagaa ggaaatgctt aacgatttcg ggtgtgagtt 6421 gacaaggaga gagagaaaag aagaggaaag gtaattcggg gacggtggtc ttttataccc 6481 ttggctaaag tcccaaccac aaagcaaaaa aattttcagt agtctatttt gcgtccggca 6541 tgggttaccc ggatggccag acaaagaaac tagtacaaag tctgaacaag cgtagattcc 6601 agactgcagt accctacgcc cttaacggca agtgtgggaa ccgggggagg tttgatatgt 6661 ggggtgaagg gggctctcgc cggggttggg cccgctactg ggtcaatttg gggtcaattg 6721 gggcaattgg ggctgttttt tgggacacaa atacgccgcc aacccggtct ctgtttaaac 6781 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 6841 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 6901 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 6961 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 7021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 7081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 7141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 7201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 7261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7381 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 7501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 7561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7621 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7801 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7861 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 7921 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 7981 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 8041 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 8101 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 8161 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 8221 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 8281 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8341 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8401 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 8461 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 8521 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 8581 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 8641 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 8701 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8761 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 8821 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 8881 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8941 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 9001 tatcacgagg cccagatcct ctagagtcga agcggccgct atgtctgata aaaggatgta 9061 acataggcaa gctgctcgtg agtgttgagt acgaacctta gatccaaatc acccgcaccc 9121 acggatatac ttgcttgaat atacagtagt atgcggccgc ttcgacacca tatcatataa 9181 aactaacaat gcattgctta ttacgaagac tacccgttgc tatctccaca ccgttatctc 9241 cacggtccaa aggctgctca atgtgctgca tacgtaacgt ggggtgcaac cttgagcaca 9301 tagtactttt ccgaaaaccg gcgataatta agtgtgcact ccaacttttc acactgagcg 9361 taaaatgtgg agaagaaatc ggcactaaaa agtcaggtag actggaaaat gcgccatgaa 9421 atgaatatct cttgctacag taatgcccag catcgagggg tattgtgtca ccaacactat 9481 agtggcagct gaagcgctcg tgattgtagt atgagtcttt attggtgatg ggaagagttc 9541 actcaatatt ctcgttactg ccaaaacacc acggtaatcg gccagacacc atggatgtag 9601 atcaccaagc ctgtgaatgt tattcgagct aaaatgcaca tggttggtga aaggagtagt 9661 tgctgtcgaa ttccgtcgtc gcctgagtca tcatttattt accagttggc cacaaaccct 9721 tgacgatctc gtatgtcccc tccgacatac tcccggccgg ctggggtacg ttcgatagcg 9781 ctatcggcat cgacaaggtt tgggtcccta gccgataccg cactacctga gtcacaatct 9841 tcggaggttt agtcttccac atagcacggg caaaagtgcg tatatataca agagcgtttg 9901 ccagccacag attttcactc cacacaccac atcacacata caaccacaca catccacaat 9961 ggaacccgaa actaagaaga ccaagactga ctccaagaag attgttcttc tcggcggcga 10021 cttctgtggc cccgaggtga ttgccgaggc cgtcaaggtg ctcaagtctg ttgctgaggc 10081 ctccggcacc gagtttgtgt ttgaggaccg actcattgga ggagctgcca ttgagaagga 10141 gggcgagccc atcaccgacg ctactctcga catctgccga aaggctgact ctattatgct 10201 cggtgctgtc ggaggcgctg ccaacaccgt atggaccact cccgacggac gaaccgacgt 10261 gcgacccgag cagggtctcc tcaagctgcg aaaggacctg aacctgtacg ccaacctgcg 10321 accctgccag ctgctgtcgc ccaagctcgc cgatctctcc cccatccgaa acgttgaggg 10381 caccgacttc atcattgtcc gagagctcgt cggaggtatc tactttggag agcgaaagga 10441 ggatgacgga tctggcgtcg cttccgacac cgagacctac tccgttcctg aggttgagcg 10501 aattgcccga atggccgcct tcctggccct tcagcacaac ccccctcttc ccgtgtggtc 10561 tcttgacaag gccaacgtgc tggcctcctc tcgactttgg cgaaagactg tcactcgagt 10621 cctcaaggac gaattccccc agctcgagct caaccaccag ctgatcgact cggccgccat 10681 gatcctcatc aagcagccct ccaagatgaa tggtatcatc atcaccacca acatgtttgg 10741 cgatatcatc tccgacgagg cctccgtcat ccccggttct ctgggtctgc tgccctccgc 10801 ctctctggct tctctgcccg acaccaacga ggcgttcggt ctgtacgagc cctgtcacgg 10861 atctgccccc gatctcggca agcagaaggt caaccccatt gccaccattc tgtctgccgc 10921 catgatgctc aagttctctc ttaacatgaa gcccgccggt gacgctgttg aggctgccgt 10981 caaggagtcc gtcgaggctg gtatcactac cgccgatatc ggaggctctt cctccacctc 11041 cgaggtcgga gacttgttgc caacaaggtc aaggagctgc tcaagaagga gtaagtcgtt 11101 tctacgacgc attgatggaa ggagcaaact gacgcgcctg cgggttggtc taccggcagg 11161 gtccgctagt gtataagact ctataaaaag ggccctgccc tgctaatgaa atgatgattt 11221 ataatttacc ggtgtagcaa ccttgactag aagaagcaga ttgggtgtgt ttgtagtgga 11281 ggacagtggt acgttttgga aacagtcttc ttgaaagtgt cttgtctaca gtatattcac 11341 tcataacctc aatagccaag ggtgtagtcg gtttattaaa ggaagggagt tgtggctgat 11401 gtggatagat atctttaagc tggcgactgc acccaacgag tgtggtggta gcttgttact 11461 gtatattcgg taagatatat tttgtggggt tttagtggtg tttggtaggt tagtgcttgg 11521 tatatgagtt gtaggcatga caatttggaa aggggtggac tttgggaata ttgtgggatt 11581 tcaatacctt agtttgtaca gggtaattgt tacaaatgat acaaagaact gtatttcttt 11641 tcatttgttt taattggttg tatatcaagt ccgttagacg agctcagtgc catggctttt 11701 ggcactgtat ttcattttta gaggtacact acattcagtg aggtatggta aggttgaggg 11761 cataatgaag gcaccttgta ctgacagtca cagacctctc accgagaatt ttatgagata 11821 tactcgggtt cattttaggc tccgattcga ttcaaattat tactgtcgaa atcggttgag 11881 catccgttga tttccgaaca gatctggccc tttcgtc //