LOCUS       pCfB2899\(X-2\MarkerFree)        4458 bp    DNA     circular UNA  7-AUG-2015
ACCESSION   BGHA URA3 ADH1 CYC1 Nb.BsmI
SOURCE      Saccharomyces cerevisiae (unknown).
  ORGANISM  Saccharomyces cerevisiae
            Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|741616396|
COMMENT     VNTDBDATE|741616396|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB2899 (X-2 MarkerFree)|
COMMENT     VNTAUTHORNAME|Mathew Jessop Fabre|
COMMENT     VNTAUTHOREML|mmjf@biosustain.dtu.dk|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/BGHA\">BGHA</a>; <a href=\"ncbilink:dnarna/URA3\">URA3</a>; <a href=\"ncbilink:dnarna/ADH1\">ADH1</a>; <a href=\"ncbilink:dnarna/CYC1\">CYC1</a>; <a href=\"ncbilink:dnarna/Nb.BsmI\">Nb.BsmI</a>" 
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COMMENT                          "Sequence Source: Saccharomyces cerevisiae (unknown)." 
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/Saccharomyces+cerevisiae\">Saccharomyces cerevisiae(Saccharomyces cerevisiae)</a>" 
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COMMENT                          "Taxonomy: Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces" 
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FEATURES             Location/Qualifiers
     gene            complement(1..859)
                     /vntifkey="60"
     primer          1167..1186
                     /vntifkey="27"
                     /label=BGHA\Pac1\F2
                     /note="PCR on col primer"
     primer_bind     1219..1238
                     /vntifkey="28"
                     /label=BGHA\F1
     misc_feature    complement(1304..1310)
                     /vntifkey="21"
                     /label=Nt.BvcI
     misc_feature    1856..1862
                     /vntifkey="21"
                     /label=Nt.BvCI
     misc_binding    1862..1873
                     /vntifkey="20"
                     /label=MCS\1
                     /note="Oriinal location: 1329..1371"
     misc_feature    1874..1879
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Nb.BsmI"
     terminator      complement(1887..2081)
                     /vntifkey="43"
                     /label=T\ADH1
     primer_bind     complement(1905..1924)
                     /vntifkey="28"
                     /label=BGHA\Pac1\R3
                     /note="Primer: GGTCAGGTTGCTTTCTCAGG Number of mismatches: 0  Primer binding region: complement(1373..1392)"
     misc_feature    2087..2092
                     /vntifkey="21"
                     /label=Misc_Feature_2
                     /note="Nb.BsmI"
     misc_feature    2105..2110
                     /vntifkey="21"
                     /label=Misc_Feature_3
                     /note="Nb.BsmI"
     terminator      2111..2300
                     /vntifkey="43"
                     /label=T\CYC1
     primer_bind     2315..2323
                     /vntifkey="28"
                     /label=BGHA\Pac2\F2
                     /note="Primer: GGTACCCAATTCGCCCTAT Number of mismatches: 0  Primer binding region: 3360..3378"
     misc_feature    2410..2416
                     /vntifkey="21"
                     /label=Nt.BvcI
     misc_feature    2921..2927
                     /vntifkey="21"
                     /label=Nt.BvcI
     primer_bind     complement(2993..3012)
                     /vntifkey="28"
                     /label=BGHA\Pac2\R
                     /note="Primer: TCGCCCTTATTCGACTCACT Number of mismatches: 0  Primer binding region: complement(3556..3575)"
     rep_origin      complement(3713..4356)
                     /vntifkey="33"
                     /label=pUC\ori
     CDS             complement(1..859)
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    2282..2300
                     /vntifkey="21"
                     /label=primer\1
     misc_feature    2315..2332
                     /vntifkey="21"
                     /label=primer\2
     misc_feature    2424..2913
                     /vntifkey="21"
                     /label=X-2\DOWN
     misc_feature    1318..1848
                     /vntifkey="21"
                     /label=X-2\UP
BASE COUNT     1192 a      1023 c       965 g      1278 t 
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcgt 
     1321 ctatgaggag actgttagtt ggatatcagt aatgagacga aaaagctcga aatgaatgga 
     1381 tatattcttt ttgctactgg caactgttga atatttaatg ttaaaacaaa ctaactgagg 
     1441 tatattcgta tctgtatgta cacatatact atatacagga aaagataagc aagagagagg 
     1501 atatcaacta cgagagcgat cgattatata tcaaaagctg tccgctttgc cacccataat 
     1561 cggcgcttag tttcggagtt caatcataat tctaccacct tacactcaac ttactcttta 
     1621 actcctatag tataatatcg ccactgaccc catattaaaa aatttttttg ctcgatcttc 
     1681 tatcctcttt aggttaattg tcgctgttat tgtctagatt ttttctcgga gatggcgcat 
     1741 ctatttgccg tcaaaagatc ctctcatacc atattaagta aattgcctcc atttcttttt 
     1801 cctcgggcag agaaactcgc aggcaacttg ctctcgaagt ggtcacgtat taagtcctca 
     1861 gcgagctcgc atggaatgcg tgcgatgagc gacctcatgc tatacctgag aaagcaacct 
     1921 gacctacagg aaagagttac tcaagaataa gaattttcgt tttaaaacct aagagtcact 
     1981 ttaaaatttg tatacactta ttttttttat aacttattta ataataaaaa tcataaatca 
     2041 taagaaattc gcttatttag aagtgtcaac aacgtatcta ccaacggaat gcgtgcgatc 
     2101 gcgtgcattc atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc 
     2161 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc 
     2221 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga 
     2281 aggttttggg acgctcgaag atcgcgtacc caattcgccc tatagtgagt cgtattacgc 
     2341 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccctgc 
     2401 aggactagtg ctgaggcatt aatcctgcat aatcggcctc acagagggat cccgttaccc 
     2461 atctatgctg aagatttatc atactattcc tccgctcgtt tcttttttca gtgaggtgtg 
     2521 tcgtgaaaga aaacccacaa ttaaactttc aacaaccggg cgactaggaa gagagtagtg 
     2581 ggcgcggatg acgaaggcta aggtcacttc ttcgtttcct ttattggggt ttccgtgtag 
     2641 ccttcccctg aatagtgtgg gacgttttat gagaagccgt aagaaatagg caaattgagt 
     2701 tatgacaagt agacatgatg ccgcagcctt gcctgacttt acgtctcctt catgaataag 
     2761 tttttctatc gagttctttt ccttttttcg ccttaattag ctcaattaag cctgtcctca 
     2821 ctacttttct ttttcttatc ggctttgtgc cacacctaac cttcgaatgc tgttttattc 
     2881 cgttcttaca tgggatggta atgccttggc gagattaaga cctcagcgcg gccgcaaatt 
     2941 taaataaaat gaagtgaagt tcctatactt tctagagaat aggaacttct atagtgagtc 
     3001 gaataagggc gacacaaaat ttattctaaa tgcataataa atactgataa catcttatag 
     3061 tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt 
     3121 ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta 
     3181 tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa 
     3241 aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc 
     3301 tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa 
     3361 actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt 
     3421 actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt tttacaacac 
     3481 agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc ttagtttgat 
     3541 gcctggcagt tccctactct cgccttccgc ttcctcgctc actgactcgc tgcgctcggt 
     3601 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     3661 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     3721 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     3781 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     3841 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     3901 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     3961 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     4021 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     4081 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     4141 tacagagttc ttgaagtggt gggctaacta cggctacact agaagaacag tatttggtat 
     4201 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     4261 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     4321 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     4381 cgcgcgcgta actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca 
     4441 gcgtaatgct ctgctttt 
//
